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AB289740

Recombinant Human NADPH oxidase 4 protein

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Recombinant Human NADPH oxidase 4 protein is a Human Full Length protein, in the 1 to 578 aa range, expressed in Cell free, with >85%, suitable for SDS-PAGE.

View Alternative Names

RENOX, NOX4, NADPH oxidase 4, Kidney oxidase-1, Kidney superoxide-producing NADPH oxidase, Renal NAD(P)H-oxidase, KOX-1

1 Images
SDS-PAGE - Recombinant Human NADPH oxidase 4 protein (AB289740)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human NADPH oxidase 4 protein (AB289740)

SDS-PAGE analysis of ab289740

Key facts

Purity

>85% SDS-PAGE

Expression system

Cell free

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q9NPH5

Animal free

Yes

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 - 8 Constituents: 6% Trehalose, 0.87% Sodium chloride, 0.24% Tris, 0.05% dodecyl 2-(trimethylazaniumyl)ethyl phosphate

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Recombinant Human ALDR1 Protein

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Proteins & Peptides

AB179799

NADPH oxidase 4 peptide

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AB191946

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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  • 20
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MAVSWRSWLANEGVKHLCLFIWLSMNVLLFWKTFLLYNQGPEYHYLHQMLGLGLCLSRASASVLNLNCSLILLPMCRTLLAYLRGSQKVPSRRTRRLLDKSRTFHITCGVTICIFSGVHVAAHLVNALNFSVNYSEDFVELNAARYRDEDPRKLLFTTVPGLTGVCMVVVLFLMITASTYAIRVSNYDIFWYTHNLFFVFYMLLTLHVSGGLLKYQTNLDTHPPGCISLNRTSSQNISLPEYFSEHFHEPFPEGFSKPAEFTQHKFVKICMEEPRFQANFPQTWLWISGPLCLYCAERLYRYIRSNKPVTIISVMSHPSDVMEIRMVKENFKARPGQYITLHCPSVSALENHPFTLTMCPTETKATFGVHLKIVGDWTERFRDLLLPPSSQDSEILPFIQSRNYPKLYIDGPFGSPFEESLNYEVSLCVAGGIGVTPFASILNTLLDDWKPYKLRRLYFIWVCRDIQSFRWFADLLCMLHNKFWQENRPDYVNIQLYLSQTDGIQKIIGEKYHALNSRLFIGRPRWKLLFDEIAKYNRGKTVGVFCCGPNSLSKTLHKLSNQNNSYGTRFEYNKESFS","proteinLength":"Full Length","predictedMolecularWeight":"69.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":578,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Cell free","accessionNumber":"Q9NPH5","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NADPH oxidase 4 also known as NOX4 is an enzyme that catalyzes the production of reactive oxygen species (ROS) by transferring electrons from NADPH to molecular oxygen. NOX4 has a molecular weight of approximately 67 kDa. It is expressed in various tissues including the kidneys lungs and heart with particularly high expression in vascular smooth muscle cells and endothelial cells. The protein serves as a homo- or heterodimer and is distinct due to its ability to produce hydrogen peroxide directly.
Biological function summary

NADPH oxidase 4 plays a role in cellular signaling and oxidative stress responses. The enzyme is part of the NADPH oxidase family which consists of several members each forming complexes with regulatory subunits such as p22phox NOXO1 or NOXA1. These complexes contribute to NOX4's stable and constitutive activity impacting cell growth differentiation and apoptosis processes. Its persistent activation suggests it modulates basal ROS levels acting as a control point in redox-dependent cell signaling.

Pathways

NOX4 is intricately involved in the TGF-beta signaling pathway and the regulation of endothelial nitric oxide synthase (eNOS) activity. The TGF-beta signaling involves NOX4 in fibrotic responses and cellular transformations often intersecting with other proteins like Smad3. In the nitric oxide signaling pathway interactions with eNOS affect the bioavailability of nitric oxide impacting vascular function. These relationships indicate NOX4's role in managing oxidative stress and maintaining vascular homeostasis through specific signaling cascades involving these proteins.

NADPH oxidase 4 contributes significantly to the pathogenesis of idiopathic pulmonary fibrosis and atherosclerosis. In idiopathic pulmonary fibrosis excessive ROS production by NOX4 leads to fibroblast differentiation and tissue remodeling. NOX4's involvement in atherosclerosis relates to overproduction of vascular ROS which affects endothelial function and promotes inflammatory processes. The protein's interaction with TGF-beta in fibrosis and eNOS in vascular disorders emphasizes its role in influencing disease progression through pathways linked to oxidative damage.
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Specifications

Form

Lyophilized

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General info

Function

NADPH oxidase that catalyzes predominantly the reduction of oxygen to H2O2 (PubMed : 14966267, PubMed : 15356101, PubMed : 15927447, PubMed : 21343298, PubMed : 25062272). Can also catalyze to a smaller extent, the reduction of oxygen to superoxide (PubMed : 10869423, PubMed : 11032835, PubMed : 15155719, PubMed : 15572675, PubMed : 15927447, PubMed : 16019190, PubMed : 16179589, PubMed : 16230378, PubMed : 16324151, PubMed : 25062272). May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity (PubMed : 16019190). May regulate insulin signaling cascade (PubMed : 14966267). May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB (PubMed : 15356101, PubMed : 15572675). May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation (PubMed : 16324151). Promotes ferroptosis, reactive oxygen species production and reduced glutathione (GSH) levels by activating NLRP3 inflammasome activation and cytokine release (PubMed : 39909992).. Isoform 4. NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor (PubMed : 15721269, PubMed : 23393389). Involved in redox signaling in vascular cells (PubMed : 23393389). Modulates the nuclear activation of ERK1/2 and the ELK1 transcription factor, and is capable of inducing nuclear DNA damage (PubMed : 23393389).. Isoform 3. Lacks superoxide-generating NADPH oxidase activity.

Post-translational modifications

Deubiquitinated by USP19.. Isoform 4. N-glycosylated and glycosylation is required for its proper function.. Isoform 3. N-glycosylated.

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Product protocols

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Target data

NADPH oxidase that catalyzes predominantly the reduction of oxygen to H2O2 (PubMed : 14966267, PubMed : 15356101, PubMed : 15927447, PubMed : 21343298, PubMed : 25062272). Can also catalyze to a smaller extent, the reduction of oxygen to superoxide (PubMed : 10869423, PubMed : 11032835, PubMed : 15155719, PubMed : 15572675, PubMed : 15927447, PubMed : 16019190, PubMed : 16179589, PubMed : 16230378, PubMed : 16324151, PubMed : 25062272). May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity (PubMed : 16019190). May regulate insulin signaling cascade (PubMed : 14966267). May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB (PubMed : 15356101, PubMed : 15572675). May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation (PubMed : 16324151). Promotes ferroptosis, reactive oxygen species production and reduced glutathione (GSH) levels by activating NLRP3 inflammasome activation and cytokine release (PubMed : 39909992).. Isoform 4. NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor (PubMed : 15721269, PubMed : 23393389). Involved in redox signaling in vascular cells (PubMed : 23393389). Modulates the nuclear activation of ERK1/2 and the ELK1 transcription factor, and is capable of inducing nuclear DNA damage (PubMed : 23393389).. Isoform 3. Lacks superoxide-generating NADPH oxidase activity.
See full target information NOX4
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