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AB222442

Recombinant Human NAGA protein (His tag)

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Recombinant Human NAGA protein (His tag) is a Human Full Length protein, in the 18 to 411 aa range, expressed in Baculovirus infected insect cells, with >90%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

View Alternative Names

Alpha-N-acetylgalactosaminidase, Alpha-galactosidase B, NAGA

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SDS-PAGE - Recombinant Human NAGA protein (His tag) (AB222442)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human NAGA protein (His tag) (AB222442)

ab222442 (3 μg) was analysed by 15% SDS-PAGE.

Predicted molecular mass : 45.5 kDa

Key facts

Purity

>90% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Baculovirus infected insect cells

Tags

His tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P17050

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: PBS, 10% Glycerol (glycerin, glycerine)

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"LDNGLLQTPPMGWLAWERFRCNINCDEDPKNCISEQLFMEMADRMAQDGWRDMGYTYLNIDDCWIGGRDASGRLMPDPKRFPHGIPFLADYVHSLGLKLGIYADMGNFTCMGYPGTTLDKVVQDAQTFAEWKVDMLKLDGCFSTPEERAQGYPKMAAALNATGRPIAFSCSWPAYEGGLPPRVNYSLLADICNLWRNYDDIQDSWWSVLSILNWFVEHQDILQPVAGPGHWNDPDMLLIGNFGLSLEQSRAQMALWTVLAAPLLMSTDLRTISAQNMDILQNPLMIKINQDPLGIQGRRIHKEKSLIEVYMRPLSNKASALVFFSCRTDMPYRYHSSLGQLNFTGSVIYEAQDVYSGDIISGLRDETNFTVIINPSGVVMWYLYPIKNLEMSQQHHHHHH","proteinLength":"Full Length","predictedMolecularWeight":"45.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":411,"aminoAcidStart":18,"nature":"Recombinant","expressionSystem":"Baculovirus infected insect cells","accessionNumber":"P17050","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NAGA also known as lysosomal alpha-N-acetylgalactosaminidase is an enzyme that catalyzes the hydrolysis of terminal N-acetylgalactosamine residues in glycoproteins and glycolipids. This enzyme weighs approximately 44 kDa. NAGA expresses predominantly in lysosomes within various tissues including liver kidney and spleen. It is a critical component in the degradation process of glycoconjugates maintaining cellular homeostasis.
Biological function summary

NAGA participates in the breakdown of complex sugar chains converting them into simpler molecules for further cellular use or disposal. This enzyme functions as a monomer not forming any known protein complexes. Its activity is necessary for the efficient turnover of biomolecules ensuring proper cellular function and maintenance of essential metabolic processes. Disordered NAGA activity may disrupt glycoconjugate breakdown resulting in cellular dysfunctions.

Pathways

NAGA contributes to lysosomal degradation pathways importantly the glycosaminoglycan (GAG) catabolism pathway. It works alongside other enzymes like alpha-N-acetylglucosaminidase and beta-galactosidase that sequentially degrade GAGs. NAGA’s function within the lysosomal degradation pathway highlights its role in maintaining cellular waste processing and recycling. Abnormalities in these pathways can affect cell health and dynamics emphasizing the enzyme's role in cellular metabolism.

NAGA is directly linked to Schindler disease and Kanzaki disease both resulting from enzyme deficiencies. In Schindler disease mutations in the NAGA gene impair glycolipid metabolism accumulating toxic substances in tissues. There is no direct interaction with other lysosomal storage disorder proteins but the disruption here can have overlapping symptoms with related disorders. Understanding NAGA’s role helps develop therapeutic strategies to mitigate enzyme deficiency effects in affected individuals.
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Specifications

Form

Liquid

Additional notes

Affinity purified

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General info

Function

Removes terminal alpha-N-acetylgalactosamine residues from glycolipids and glycopeptides. Required for the breakdown of glycolipids.

Sequence similarities

Belongs to the glycosyl hydrolase 27 family.

Subcellular localisation

Lysosome

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Product protocols

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Target data

Removes terminal alpha-N-acetylgalactosamine residues from glycolipids and glycopeptides. Required for the breakdown of glycolipids.
See full target information Alpha-N-acetylgalactosaminidase
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