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AB124563

Recombinant Human NAGK protein (His tag N-Terminus)

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Recombinant Human NAGK protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 344 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

N-acetyl-D-glucosamine kinase, N-acetylglucosamine kinase, GlcNAc kinase, Muramyl dipeptide kinase, N-acetyl-D-mannosamine kinase, NAGK

1 Images
SDS-PAGE - Recombinant Human NAGK protein (His tag N-Terminus) (AB124563)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human NAGK protein (His tag N-Terminus) (AB124563)

ab124563 (3µg) visualised by SDS-PAGE (15%).

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q9UJ70

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 1.16% Sodium chloride, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMAAIYGGVEGGGTRSEVLLVSEDGKILAEADGLSTNHWLIGTDKCVERINEMVNRAKRKAGVDPLVPLRSLGLSLSGGDQEDAGRILIEELRDRFPYLSESYLITTDAAGSIATATPDGGVVLISGTGSNCRLINPDGSESGCGGWGHMMGDEGSAYWIAHQAVKIVFDSIDNLEAAPHDIGYVKQAMFHYFQVPDRLGILTHLYRDFDKCRFAGFCRKIAEGAQQGDPLSRYIFRKAGEMLGRHIVAVLPEIDPVLFQGKIGLPILCVGSVWKSWELLKEGFLLALTQGREIQAQNFFSSFTLMKLRHSSALGGASLGARHIGHLLPMDYSANAIAFYSYTFS","proteinLength":"Full Length","predictedMolecularWeight":"39.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":344,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9UJ70","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

N-Acetylglucosamine Kinase (NAGK) also known as GlcNAc kinase catalyzes the phosphorylation of N-acetylglucosamine to N-acetylglucosamine-6-phosphate. It has a molecular mass of approximately 37 kDa. NAGK expresses in various tissues prominently within the liver and pancreas indicating its involvement in metabolic functions related to these organs.
Biological function summary

NAGK plays a significant role in amino sugar and nucleotide sugar metabolism. It does not form part of a large protein complex but interacts with other metabolic enzymes to carry out its function. By converting N-acetylglucosamine to its phosphorylated form NAGK helps to maintain cellular energy balance and provides substrates necessary for glycosylation processes.

Pathways

The enzymatic activity of NAGK is essential in the hexosamine biosynthetic pathway. This pathway feeds into glycosylation processes necessary for protein modification and cellular communication. NAGK activity links to proteins such as UDP-N-acetylglucosamine pyrophosphorylase in these pathways highlighting its role in maintaining cellular function and integrity.

Alterations in NAGK function relate to metabolic syndromes and potential glycosylation disorders. Changes in NAGK could affect pathways involving N-linked glycosylation which plays a role in diseases like congenital disorders of glycosylation. Proteins such as glutamine fructose-6-phosphate amidotransferase may also interact with NAGK in the context of these diseases affecting broader metabolic processes.
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Specifications

Form

Liquid

Additional notes

Purified using conventional chromatography techniques.

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General info

Function

Converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate (PubMed : 22692205). Involved in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway : although human is not able to catalyze formation of Neu5Gc due to the inactive CMAHP enzyme, Neu5Gc is present in food and must be degraded (PubMed : 22692205). Also has N-acetylmannosamine (ManNAc) kinase activity (By similarity). Also involved in innate immunity by promoting detection of bacterial peptidoglycan by NOD2 : acts by catalyzing phosphorylation of muramyl dipeptide (MDP), a fragment of bacterial peptidoglycan, to generate 6-O-phospho-muramyl dipeptide, which acts as a direct ligand for NOD2 (PubMed : 36002575).

Sequence similarities

Belongs to the eukaryotic-type N-acetylglucosamine kinase family.

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Product protocols

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Target data

Converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate (PubMed : 22692205). Involved in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway : although human is not able to catalyze formation of Neu5Gc due to the inactive CMAHP enzyme, Neu5Gc is present in food and must be degraded (PubMed : 22692205). Also has N-acetylmannosamine (ManNAc) kinase activity (By similarity). Also involved in innate immunity by promoting detection of bacterial peptidoglycan by NOD2 : acts by catalyzing phosphorylation of muramyl dipeptide (MDP), a fragment of bacterial peptidoglycan, to generate 6-O-phospho-muramyl dipeptide, which acts as a direct ligand for NOD2 (PubMed : 36002575).
See full target information NAGK
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