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Recombinant human NAK/TBK1 protein is a Human Full Length protein, in the 1 to 729 aa range, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, WB, FuncS.
Alternative names=Serine/threonine-protein kinase TBK1, NF-kappa-B-activating kinase, T2K, TANK-binding kinase 1, NAK, TBK1
- Purity
>70%
- Expression system
Baculovirus infected Sf9 cells
- Tags
GST tag N-Terminus
- Applications
SDS-PAGE, WB, FuncS
- Biologically active
Yes
- Sequence
M Q S T S N H L W L L S D I L G Q G A T A N V F R G R H K K T G D L F A I K V F N N I S F L R P V D V Q M R E F E V L K K L N H K N I V K L F A I E E E T T T R H K V L I M E F C P C G S L Y T V L E E P S N A Y G L P E S E F L I V L R D V V G G M N H L R E N G I V H R D I K P G N I M R V I G E D G Q S V Y K L T D F G A A R E L E D D E Q F V S L Y G T E E Y L H P D M Y E R A V L R K D H Q K K Y G A T V D L W S I G V T F Y H A A T G S L P F R P F E G P R R N K E V M Y K I I T G K P S G A I S G V Q K A E N G P I D W S G D M P V S C S L S R G L Q V L L T P V L A N I L E A D Q E K C W G F D Q F F A E T S D I L H R M V I H V F S L Q Q M T A H K I Y I H S Y N T A T I F H E L V Y K Q T K I I S S N Q E L I Y E G R R L V L E P G R L A Q H F P K T T E E N P I F V V S R E P L N T I G L I Y E K I S L P K V H P R Y D L D G D A S M A K A I T G V V C Y A C R I A S T L L L Y Q E L M R K G I R W L I E L I K D D Y N E T V H K K T E V V I T L D F C I R N I E K T V K V Y E K L M K I N L E A A E L G E I S D I H T K L L R L S S S Q G T I E T S L Q D I D S R L S P G G S L A D A W A H Q E G T H P K D R N V E K L Q V L L N C M T E I Y Y Q F K K D K A E R R L A Y N E E Q I H K F D K Q K L Y Y H A T K A M T H F T D E C V K K Y E A F L N K S E E W I R K M L H L R K Q L L S L T N Q C F D I E E E V S K Y Q E Y T N E L Q E T L P Q K M F T A S S G I K H T M T P I Y P S S N T L V E M T L G M K K L K E E M E G V V K E L A E N N H I L E R F G S L T M D G G L R N V D C L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes Ab85276 can be used as a WB positive control in conjunction with ab75969. |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Recombinant human NAK/TBK1 protein is a Human Full Length protein, in the 1 to 729 aa range, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, WB, FuncS.
Alternative names=Serine/threonine-protein kinase TBK1, NF-kappa-B-activating kinase, T2K, TANK-binding kinase 1, NAK, TBK1
Key facts
- Purity
>70%
- Expression system
Baculovirus infected Sf9 cells
- Tags
GST tag N-Terminus
- Applications
SDS-PAGE, WB, FuncS
- Biologically active
Yes
- Biological activity
The Specific activity of ab85276 was determined to be 305 nmol/min/mg.
- Predicted molecular weight
105 kDa
- Amino acids
1 to 729
- Sequence
M Q S T S N H L W L L S D I L G Q G A T A N V F R G R H K K T G D L F A I K V F N N I S F L R P V D V Q M R E F E V L K K L N H K N I V K L F A I E E E T T T R H K V L I M E F C P C G S L Y T V L E E P S N A Y G L P E S E F L I V L R D V V G G M N H L R E N G I V H R D I K P G N I M R V I G E D G Q S V Y K L T D F G A A R E L E D D E Q F V S L Y G T E E Y L H P D M Y E R A V L R K D H Q K K Y G A T V D L W S I G V T F Y H A A T G S L P F R P F E G P R R N K E V M Y K I I T G K P S G A I S G V Q K A E N G P I D W S G D M P V S C S L S R G L Q V L L T P V L A N I L E A D Q E K C W G F D Q F F A E T S D I L H R M V I H V F S L Q Q M T A H K I Y I H S Y N T A T I F H E L V Y K Q T K I I S S N Q E L I Y E G R R L V L E P G R L A Q H F P K T T E E N P I F V V S R E P L N T I G L I Y E K I S L P K V H P R Y D L D G D A S M A K A I T G V V C Y A C R I A S T L L L Y Q E L M R K G I R W L I E L I K D D Y N E T V H K K T E V V I T L D F C I R N I E K T V K V Y E K L M K I N L E A A E L G E I S D I H T K L L R L S S S Q G T I E T S L Q D I D S R L S P G G S L A D A W A H Q E G T H P K D R N V E K L Q V L L N C M T E I Y Y Q F K K D K A E R R L A Y N E E Q I H K F D K Q K L Y Y H A T K A M T H F T D E C V K K Y E A F L N K S E E W I R K M L H L R K Q L L S L T N Q C F D I E E E V S K Y Q E Y T N E L Q E T L P Q K M F T A S S G I K H T M T P I Y P S S N T L V E M T L G M K K L K E E M E G V V K E L A E N N H I L E R F G S L T M D G G L R N V D C L
- Accession
- Q9UHD2-1
- Protein length
Full Length
- Animal free
No
- Nature
Recombinant
- Species
Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF
Specifications
- Form
Liquid
- Additional notes
Purified by affinity chromatography
General info
- Function
Serine/threonine kinase that plays an essential role in regulating inflammatory responses to foreign agents (PubMed:12692549, PubMed:14703513, PubMed:18583960, PubMed:12702806, PubMed:15367631, PubMed:10581243, PubMed:11839743, PubMed:15485837, PubMed:21138416, PubMed:25636800, PubMed:23453971, PubMed:23453972, PubMed:23746807, PubMed:26611359, PubMed:32404352). Following activation of toll-like receptors by viral or bacterial components, associates with TRAF3 and TANK and phosphorylates interferon regulatory factors (IRFs) IRF3 and IRF7 as well as DDX3X (PubMed:12692549, PubMed:14703513, PubMed:18583960, PubMed:12702806, PubMed:15367631, PubMed:25636800). This activity allows subsequent homodimerization and nuclear translocation of the IRFs leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNA and IFNB (PubMed:12702806, PubMed:15367631, PubMed:25636800, PubMed:32972995). In order to establish such an antiviral state, TBK1 form several different complexes whose composition depends on the type of cell and cellular stimuli (PubMed:23453971, PubMed:23453972, PubMed:23746807). Plays a key role in IRF3 activation: acts by first phosphorylating innate adapter proteins MAVS, STING1 and TICAM1 on their pLxIS motif, leading to recruitment of IRF3, thereby licensing IRF3 for phosphorylation by TBK1 (PubMed:25636800, PubMed:30842653). Phosphorylated IRF3 dissociates from the adapter proteins, dimerizes, and then enters the nucleus to induce expression of interferons (PubMed:25636800). Thus, several scaffolding molecules including FADD, TRADD, MAVS, AZI2, TANK or TBKBP1/SINTBAD can be recruited to the TBK1-containing-complexes (PubMed:21931631). Under particular conditions, functions as a NF-kappa-B effector by phosphorylating NF-kappa-B inhibitor alpha/NFKBIA, IKBKB or RELA to translocate NF-Kappa-B to the nucleus (PubMed:10783893, PubMed:15489227). Restricts bacterial proliferation by phosphorylating the autophagy receptor OPTN/Optineurin on 'Ser-177', thus enhancing LC3 binding affinity and antibacterial autophagy (PubMed:21617041). Phosphorylates SMCR8 component of the C9orf72-SMCR8 complex, promoting autophagosome maturation (PubMed:27103069). Phosphorylates and activates AKT1 (PubMed:21464307). Seems to play a role in energy balance regulation by sustaining a state of chronic, low-grade inflammation in obesity, wich leads to a negative impact on insulin sensitivity (By similarity). Attenuates retroviral budding by phosphorylating the endosomal sorting complex required for transport-I (ESCRT-I) subunit VPS37C (PubMed:21270402). Phosphorylates Borna disease virus (BDV) P protein (PubMed:16155125). Plays an essential role in the TLR3- and IFN-dependent control of herpes virus HSV-1 and HSV-2 infections in the central nervous system (PubMed:22851595).
- Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
- Post-translational modifications
Autophosphorylation at Ser-172 activates the kinase, and is an essential step for virus-triggered signaling. Phosphorylated by IKBKB/IKKB at Ser-172. Phosphorylation requires homodimerization and ubiquitination at Lys-30 and Lys-401. Dephosphorylated at Ser-172 by PPM1B and this negatively regulates its role in mediating antiviral response.
Storage
- Shipped at conditions
Dry Ice
- Appropriate long-term storage conditions
-80°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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