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AB101109

Recombinant Human NANS protein

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Recombinant Human NANS protein is a Human Full Length protein, in the 1 to 359 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

SAS, NANS, Sialic acid synthase, N-acetylneuraminate synthase, N-acetylneuraminate-9-phosphate synthase, N-acetylneuraminic acid phosphate synthase, N-acetylneuraminic acid synthase

1 Images
SDS-PAGE - Recombinant Human NANS protein (AB101109)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human NANS protein (AB101109)

15% SDS-PAGE analysis of 3μg ab101109.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q9NR45

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMPLELELCPGRWVGGQHPCFIIAEIGQNHQGDLDVAKRMIRMAKECGADCAKFQKSELEFKFNRKALERPYTSKHSWGKTYGEHKRHLEFSHDQYRELQRYAEEVGIFFTASGMDEMAVEFLHELNVPFFKVGSGDTNNFPYLEKTAKKGRPMVISSGMQSMDTMKQVYQIVKPLNPNFCFLQCTSAYPLQPEDVNLRVISEYQKLFPDIPIGYSGHETGIAISVAAVALGAKVLERHITLDKTWKGSDHSASLEPGELAELVRSVRLVERALGSPTKQLLPCEMACNEKLGKSVVAKVKIPEGTILTMDMLTVKVGEPKGYPPEDIFNLVGKKVLVTVEEDDTIMEELVDNHGKKIKS","proteinLength":"Full Length","predictedMolecularWeight":"42.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":359,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9NR45","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

N-acetylneuraminate synthase commonly known as NANS catalyzes the formation of N-acetylneuraminic acid (Neu5Ac) from N-acetyl-D-mannosamine 6-phosphate and phosphoenolpyruvate. This process is important in the sialic acid biosynthesis pathway. NANS has a molecular weight of approximately 46 kDa and is expressed in various tissues most abundantly in the liver and brain. These tissues show significant metabolic activity suggesting the enzyme's active involvement in cellular processes.
Biological function summary

This enzyme plays a significant role in the synthesis of sialic acids which are essential components of glycoconjugates such as glycoproteins and glycolipids. NANS is not known to associate with any specific large protein complexes but its action is necessary for generating substrates that contribute to protein glycosylation and the modification of lipids. These modifications influence cell-cell communication neurite outgrowth and immune response modulation.

Pathways

This enzyme is pivotal in the sialic acid biosynthesis pathway impacting the production of glycoproteins and glycolipids. In this context NANS interacts with key enzymes like sialyltransferases which further transfer sialic acids to the proteins and lipids. This pathway is essential in glycobiology affecting cellular interactions and signal transduction processes.

Mutations in the NANS gene have been linked to developmental delay syndromes especially NANS deficiency. These symptoms appear due to disrupted sialic acid production leading to abnormal glycosylation patterns. Furthermore the alteration in NANS activity indirectly affects proteins such as integrins and selectins contributing to immune deficiencies and metabolic syndromes.
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Specifications

Form

Liquid

Additional notes

ab101109 was purified by using conventional chromatography techniques.

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General info

Function

Produces N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN) (PubMed : 10749855, PubMed : 27213289). Can also use N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of Neu5Ac and KDN, respectively (PubMed : 10749855).

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Product protocols

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Target data

Produces N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN) (PubMed : 10749855, PubMed : 27213289). Can also use N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of Neu5Ac and KDN, respectively (PubMed : 10749855).
See full target information NANS
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