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AB171686

Recombinant Human NAP1L4 protein

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Recombinant Human NAP1L4 protein is a Human Full Length protein, in the 1 to 375 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

NAP2, NAP1L4, Nucleosome assembly protein 1-like 4, Nucleosome assembly protein 2, NAP-2

1 Images
SDS-PAGE - Recombinant Human NAP1L4 protein (AB171686)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human NAP1L4 protein (AB171686)

15% SDS-PAGE analysis of ab171686 (3µg).

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q99733

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMADHSFSDGVPSDSVEAAKNASNTEKLTDQVMQNPRVLAALQERLDNVPHTPSSYIETLPKAVKRRINALKQLQVRCAHIEAKFYEEVHDLERKYAALYQPLFDKRREFITGDVEPTDAESEWHSENEEEEKLAGDMKSKVVVTEKAAATAEEPDPKGIPEFWFTIFRNVDMLSELVQEYDEPILKHLQDIKVKFSDPGQPMSFVLEFHFEPNDYFTNSVLTKTYKMKSEPDKADPFSFEGPEIVDCDGCTIDWKKGKNVTVKTIKKKQKHKGRGTVRTITKQVPNESFFNFFNPLKASGDGESLDEDSEFTLASDFEIGHFFRERIVPRAVLYFTGEAIEDDDNFEEGEEGEEEELEGDEEGEDEDDAEINPKV","proteinLength":"Full Length","predictedMolecularWeight":"45.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":375,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q99733","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NAP1L4 also known as nucleosome assembly protein 1-like 4 is a protein involved in nucleosome assembly. It weighs approximately 57 kDa and plays a role in shuttling histones during nucleosome assembly which is important for chromatin remodeling and DNA replication. NAP1L4 is expressed in tissues with high replication and transcriptional activities including the testis brain and various tumor tissues.
Biological function summary

NAP1L4 functions as a chaperone for histones guiding their incorporation into nucleosomes. This protein assists in the proper folding and stabilization of histones and nucleosome structure which is essential for regulating access to DNA. NAP1L4 often interacts with other chromatin assembly factors forming part of larger complexes such as with NAP1 family members to enhance nucleosome assembly efficiency during cell division.

Pathways

NAP1L4 plays a role in the process of chromatin assembly and nucleosome positioning which is a part of the larger DNA replication and repair pathways. It is functionally related to other proteins involved in these pathways such as histone H3 and H4 which NAP1L4 helps shuttle. Additionally it contributes to the regulation of gene expression by modifying chromatin structure linking it to pathways like transcription regulation.

Malfunction or altered expression of NAP1L4 can influence the development and progression of cancer and neurological disorders. NAP1L4's role in nucleosome assembly affects the regulation of gene expression potentially leading to oncogenesis when disrupted. It also ties to neurological conditions through its expression in brain tissue where it may interact with proteins related to neuronal gene regulation or misregulation such as histone acetyltransferases.
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Specifications

Form

Liquid

Additional notes

ab171686 is purified using conventional chromatography techniques.

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General info

Function

Acts as a histone chaperone in nucleosome assembly.

Sequence similarities

Belongs to the nucleosome assembly protein (NAP) family.

Post-translational modifications

Phosphorylated at the G0/G1 boundary but it is not phosphorylated in S-phase. Phosphorylated protein remains in the cytoplasm in a complex with histones during the G0/G1 transition, whereas dephosphorylation triggers its transport into the nucleus at the G1/S-boundary.. Polyglutamylated by TTLL4, a modification that occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Some residues may also be monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human (By similarity).

Subcellular localisation

Nucleus

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Product protocols

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Target data

Acts as a histone chaperone in nucleosome assembly.
See full target information NAP1L4
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