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AB207154

Recombinant Human NARS protein (His tag N-Terminus)

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Recombinant Human NARS protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 548 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

NARS, NRS, NARS1, Asparaginyl-tRNA synthetase, Asparaginyl-tRNA synthetase 1, AsnRS

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SDS-PAGE - Recombinant Human NARS protein (AB207154)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human NARS protein (AB207154)

SDS-PAGE analysis of ab207154 (3µg).

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

O43776

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: PBS, 10% Glycerol (glycerin, glycerine)

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMVLAELYVSDREGSDATGDGTKEKPFKTGLKALMTVGKEPFPTIYVDSQKENERWNVISKSQLKNIKKMWHREQMKSESREKKEAEDSLRREKNLEEAKKITIKNDPSLPEPKCVKIGALEGYRGQRVKVFGWVHRLRRQGKNLMFLVLRDGTGYLQCVLADELCQCYNGVLLSTESSVAVYGMLNLTPKGKQAPGGHELSCDFWELIGLAPAGGADNLINEESDVDVQLNNRHMMIRGENMSKILKARSMVTRCFRDHFFDRGYYEVTPPTLVQTQVEGGATLFKLDYFGEEAFLTQSSQLYLETCLPALGDVFCIAQSYRAEQSRTRRHLAEYTHVEAECPFLTFDDLLNRLEDLVCDVVDRILKSPAGSIVHELNPNFQPPKRPFKRMNYSDAIVWLKEHDVKKEDGTFYEFGEDIPEAPERLMTDTINEPILLCRFPVEIKSFYMQRCPEDSRLTESVDVLMPNVGEIVGGSMRIFDSEEILAGYKREGIDPTPYYWYTDQRKYGTCPHGGYGLGLERFLTWILNRYHIRDVCLYPRFVQRCTP","proteinLength":"Full Length","predictedMolecularWeight":"65.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":548,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O43776","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NARS also known as asparaginyl-tRNA synthetase is an enzyme with a mass of about 65 kDa. This enzyme plays an important role in protein synthesis by catalyzing the attachment of asparagine to its corresponding tRNA. NARS is expressed in various tissues across the body with noticeable levels in the liver and brain. The enzyme operates as a homodimer and requires ATP for its catalytic activity which facilitates the formation of asparaginyl-tRNA ensuring proper translation of genetic information during protein synthesis.
Biological function summary

As an essential component of the protein synthesis machinery NARS functions to ensure the fidelity and efficiency of genetic translation. It does not form part of a larger complex but its activity is critical in maintaining cellular function by facilitating accurate amino acid incorporation into growing polypeptide chains. This precise activity is necessary for cellular viability and the prevention of erroneous protein synthesis which could lead to dysfunctional proteins and cellular stress.

Pathways

NARS interacts with multiple biological pathways including the aminoacyl-tRNA biosynthesis pathway and the protein translation pathway. NARS’s role in aminoacyl-tRNA biosynthesis is important as it interacts with other aminoacyl-tRNA synthetases to maintain balanced protein synthesis. In the protein translation pathway it works alongside other elongation factors such as EF-Tu which ensures the correct delivery of aminoacyl-tRNA to the ribosome thereby orchestrating the translation process.

NARS's dysfunction links to neurodegenerative disorders and certain cancers. Mutations or aberrant expression of NARS may result in impaired protein synthesis contributing to neurological conditions such as Charcot-Marie-Tooth disease. Other proteins involved in these disorders include GARS another tRNA synthetase associated with similar hereditary neuropathies. Additionally increased expression of NARS has been noted in some cancerous tissues implicating its potential role in tumorigenesis and highlighting its relevance in cancer pathways.
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Specifications

Form

Liquid

Additional notes

ab207154 was purified using conventional chromatography techniques.

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General info

Function

Catalyzes the attachment of asparagine to tRNA(Asn) in a two-step reaction : asparagine is first activated by ATP to form Asn-AMP and then transferred to the acceptor end of tRNA(Asn) (PubMed : 32738225, PubMed : 32788587, PubMed : 9421509). In addition to its essential role in protein synthesis, acts as a signaling molecule that induced migration of CCR3-expressing cells (PubMed : 12235211, PubMed : 30171954). Has an essential role in the development of the cerebral cortex, being required for proper proliferation of radial glial cells (PubMed : 32788587).

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family.

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Product protocols

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Target data

Catalyzes the attachment of asparagine to tRNA(Asn) in a two-step reaction : asparagine is first activated by ATP to form Asn-AMP and then transferred to the acceptor end of tRNA(Asn) (PubMed : 32738225, PubMed : 32788587, PubMed : 9421509). In addition to its essential role in protein synthesis, acts as a signaling molecule that induced migration of CCR3-expressing cells (PubMed : 12235211, PubMed : 30171954). Has an essential role in the development of the cerebral cortex, being required for proper proliferation of radial glial cells (PubMed : 32788587).
See full target information NARS1
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Complementary Products

Primary Antibodies

AB129162

Anti-NARS antibody [EPR8221]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NARS antibody [EPR8221] (AB129162)

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