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AB276217

Recombinant human NBL1 protein (His tag)

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Recombinant human NBL1 protein (His tag) is a Human Full Length protein, in the 2 to 180 aa range, expressed in HEK 293 cells, with >98%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.

View Alternative Names

DAN, DAND1, NBL1, Neuroblastoma suppressor of tumorigenicity 1, DAN domain family member 1, Protein N03, Zinc finger protein DAN

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Functional Studies - Recombinant human NBL1 protein (His tag) (AB276217)
  • FuncS

Supplier Data

Functional Studies - Recombinant human NBL1 protein (His tag) (AB276217)

Measured by its ability to inhibit BMP4-induced activity in MC3T3-E1 Mouse osteoblastic cells.

The ED50 for this effect is typically 5-20 μg/ml in the presence of 50 ng/mL of recombinant human BMP4.

SDS-PAGE - Recombinant human NBL1 protein (His tag) (AB276217)
  • SDS-PAGE

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SDS-PAGE - Recombinant human NBL1 protein (His tag) (AB276217)

SDS-PAGE analysis of ab276217

Key facts

Purity

>98% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Measured by its ability to inhibit BMP4-induced activity in MC3T3-E1 Mouse osteoblastic cells.

The ED50 for this effect is typically 5-20 μg/ml in the presence of 50 ng/mL of recombinant human BMP4.

Accession

P41271

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: PBS

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MMLRVLVGAVLPAMLLAAPPPINKLALFPDKSAWCEAKNITQIVGHSGCEAKSIQNRACLGQCFSYSVPNTFPQSTESLVHCDSCMPAQSMWEIVTLECPGHEEVPRVDKLVEKILHCSCQACGKEPSHEGLSVYVQGEDGPGSQPGTHPHPHPHPHPGGQTPEPEDPPGAPHTEEEGAED","proteinLength":"Full Length","predictedMolecularWeight":"19.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":180,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P41271","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NBL1 also known as DAN domain family member 1 is a protein with a molecular weight of approximately 20 kDa. It is found in various tissues but shows higher expression levels in the nervous tissue and skeletal muscle. Mechanically NBL1 acts as a modulator in cell signaling pathways by binding to specific growth factors such as BMPs (Bone Morphogenetic Proteins) inhibiting their functions and affecting cell differentiation and growth.
Biological function summary

NBL1 plays an inhibitory role in bone morphogenesis by interacting with BMPs which are part of the TGF-beta superfamily. NBL1 can form complexes with these growth factors thereby regulating processes related to bone and cartilage development. This protein is essential for maintaining cellular processes involved in tissue homeostasis and suppressing unchecked cellular proliferation.

Pathways

NBL1 involves itself in the BMP signaling pathway where it directly impacts the regulation of bone and cartilage development. It is associated with other proteins like Noggin and Chordin both of which also modulate BMP activity highlighting its importance in balancing cell growth and differentiation signals. The involvement of NBL1 in the TGF-beta/BMP pathway is critical for embryonic development and the regulation of body patterning.

NBL1 has been linked to conditions such as osteoarthritis and certain cancer types. Its inhibitory effect on BMPs associates it with osteoarthritis where altered BMP signaling contributes to cartilage degradation. In cancer where BMP activity can be dysregulated NBL1's role in inhibiting BMP pathways places it in connection to tumor suppressors like SMAD proteins emphasizing its potential relevance in tumorigenesis and metastasis.
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Specifications

Form

Lyophilized

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General info

Function

Possible candidate as a tumor suppressor gene of neuroblastoma. May play an important role in preventing cells from entering the final stage (G1/S) of the transformation process.

Sequence similarities

Belongs to the DAN family.

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Product protocols

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Target data

Possible candidate as a tumor suppressor gene of neuroblastoma. May play an important role in preventing cells from entering the final stage (G1/S) of the transformation process.
See full target information NBL1
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