Recombinant Human NCE2/UBE2F protein (His tag N-Terminus)
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Recombinant Human NCE2/UBE2F protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 185 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.
View Alternative Names
NCE2, UBE2F, NEDD8-conjugating enzyme UBE2F, NEDD8 carrier protein UBE2F, NEDD8 protein ligase UBE2F, NEDD8-conjugating enzyme 2, RING-type E3 NEDD8 transferase UBE2F, Ubiquitin-conjugating enzyme E2 F
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant Human NCE2/UBE2F protein (His tag N-Terminus) (AB128456)
15% SDS-PAGE analysis of 3 μg ab128456.
Reactivity data
Product details
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
UBE2F functions as an important component in cellular processes by participating in the neddylation pathway. It forms a complex with NEDD8-activating E1 enzymes facilitating the transfer of NEDD8 to specific E3 ligases primarily targeting cullins. Through neddylation UBE2F influences the activation of these ligases impacting protein degradation pathways cell cycle regulation and signal transduction. This functional role positions UBE2F as an important player in maintaining cellular homeostasis.
Pathways
UBE2F integrates into the neddylation and ubiquitination pathways. Neddylation specifically modulates ubiquitin-proteasome system where NEDD8 acts as a regulator of Cullin-RING E3 ligase activity. Through these interactions UBE2F contributes to the regulation of protein turnover alongside ubiquitin itself. Connections in these pathways often involve proteins like CUL5 which works synchronously with UBE2F to ensure proper cell cycle progression and stress response in cells.
Specifications
Form
Liquid
Additional notes
ab128456 is purified using conventional chromatography techniques.
General info
Function
Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins (PubMed : 19250909, PubMed : 23201271). Together with the E3 ubiquitin ligase RNF7/RBX2, specifically neddylates cullin-5 (CUL5) (PubMed : 19250909, PubMed : 23201271, PubMed : 23300442). Does not neddylate CUL1, CUL2, CUL3, CUL4A or CUL4B (PubMed : 19250909, PubMed : 23201271). Mediates neddylation of the CUL9-RBX1 complex (PubMed : 38605244).. (Microbial infection) Following infection by HIV-1 virus, participates to HIV-1 Vif protein-mediated ubiquitination and degradation of APOBEC3G by mediating neddylation of cullin-5 (CUL5).
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family. UBE2F subfamily.
Post-translational modifications
The acetylation of Met-1 increases affinity for DCUN1D3 by about 2 orders of magnitude and is crucial for NEDD8 transfer to cullins.
Target data
Complementary Products
Product promise
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