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AB98054

Recombinant Human NCF1/p47-phox protein

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Recombinant Human NCF1/p47-phox protein is a Human Full Length protein, in the 1 to 390 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec, WB.

View Alternative Names

NOXO2, SH3PXD1A, NCF1, Neutrophil cytosol factor 1, NCF-1, 47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-47K, Neutrophil NADPH oxidase factor 1, Nox organizer 2, Nox-organizing protein 2, SH3 and PX domain-containing protein 1A, p47-phox

2 Images
Western blot - Recombinant Human NCF1/p47-phox protein (AB98054)
  • WB

Unknown

Western blot - Recombinant Human NCF1/p47-phox protein (AB98054)

All lanes:

Western blot - Anti-NCF1/p47-phox antibody (<a href='/en-us/products/primary-antibodies/ncf1-p47-phox-antibody-ab795'>ab795</a>) at 1.5 µg/mL

All lanes:

Western blot - Recombinant Human NCF1/p47-phox protein (ab98054) at 0.1 µg

Secondary

All lanes:

Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution

Predicted band size: 44 kDa

true

Exposure time: 8min

SDS-PAGE - Recombinant Human NCF1/p47-phox protein (AB98054)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human NCF1/p47-phox protein (AB98054)

3ug by SDS-PAGE under reducing conditions and visualized by coomassie blue stain.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag C-Terminus

Applications

SDS-PAGE, WB, Mass Spec

applications

Biologically active

No

Accession

P14598

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab98054 can be used as a WB positive control in conjunction with <a href='/en-us/products/primary-antibodies/ncf1-p47-phox-antibody-ab795'>ab795</a>.</p>" } } }
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Product details

Previously labelled as NCF1.
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Sequence info

[{"sequence":"MGDTFIRHIALLGFEKRFVPSQHYVYMFLVKWQDLSEKVVYRRFTEIYEFHKTLKEMFPIEAGAINPENRIIPHLPAPKWFDGQRAAENRQGTLTEYCSTLMSLPTKISRCPHLLDFFKVRPDDLKLPTDNQTKKPETYLMPKDGKSTATDITGPIILQTYRAIANYEKTSGSEMALSTGDVVEVVEKSESGWWFCQMKAKRGWIPASFLEPLDSPDETEDPEPNYAGEPYVAIKAYTAVEGDEVSLLEGEAVEVIHKLLDGWWVIRKDDVTGYFPSMYLQKSGQDVSQAQRQIKRGAPPRRSSIRNAHSIHQRSRKRLSQDAYRRNSVRFLQQRRRQARPGPQSPGSPLEEERQTQRSKPQPAVPPRPSADLILNRCSESTKRKLASAVVEHHHHHH","proteinLength":"Full Length","predictedMolecularWeight":"45.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":390,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P14598","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NCF1 also known as p47-phox p47-phox protein or NCF-1 is a cytosolic component of the NADPH oxidase complex. It has a molecular mass of about 47 kDa. NCF1/p47-phox is expressed in neutrophils monocytes and other phagocytic cells. Functionally it plays an important role in the regulation of reactive oxygen species (ROS) production by facilitating the translocation of other subunits to the cell membrane where the NADPH oxidase complex assembles and becomes active.
Biological function summary

The NCF1/p47-phox protein plays a critical role in host defense by generating ROS which are essential for microbial killing. NCF1 is an integral part of the NADPH oxidase complex along with other subunits such as p67-phox p40-phox gp91-phox and p22-phox. When stimulated NCF1 interacts with these subunits to form an active enzyme complex that transfers electrons from NADPH to oxygen producing superoxide anion and other ROS.

Pathways

The NCF1/p47-phox component participates mainly in the NADPH oxidase activation pathway and the respiratory burst pathway. NCF1 is connected to the RAC2 GTPase which upon activation stimulates the assembly and activation of the NADPH oxidase complex at the plasma membrane. This pathway is key for the innate immune response and effective clearance of pathogens by phagocytic cells.

Mutations or dysfunction in the NCF1/p47-phox gene can lead to chronic granulomatous disease (CGD) which is characterized by a failure to produce ROS and a consequent inability to kill certain bacteria and fungi. Additionally NCF1 has links to autoimmune conditions such as rheumatoid arthritis where impaired ROS production may contribute to pathogenesis. These disease implications also involve other components of the NADPH oxidase complex such as gp91-phox whose dysfunction similarly contributes to these disorders.
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Specifications

Form

Liquid

Additional notes

ab98054 was purified using conventional chromatography techniques.

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General info

Function

NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).

Post-translational modifications

Phosphorylated by PRKCD; phosphorylation induces activation of NCF1 and NADPH oxidase activity.

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Product protocols

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Target data

NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
See full target information NCF1
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Complementary Products

Primary Antibodies

AB308256

Anti-NCF1/p47-phox antibody [EPR27205-231]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-NCF1/p47-phox antibody [EPR27205-231] (AB308256)

  • 0
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