Recombinant Human Nck protein (His tag N-Terminus)
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(1 Publication)
Recombinant Human Nck protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 377 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.
View Alternative Names
NCK, NCK1, SH2/SH3 adapter protein NCK1, Cytoplasmic protein NCK1, NCK adapter protein 1, SH2/SH3 adapter protein NCK-alpha, Nck-1
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant Human Nck protein (His tag N-Terminus) (AB97409)
15% SDS-PAGE showing ab97409 at approximately 45kDa (3μg).
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The Nck proteins play significant roles in actin cytoskeleton reorganization influencing cell shape movement and adhesion. These proteins often participate as part of larger signaling complexes where they serve as scaffolds bringing together key molecules. Nck proteins directly interact with partners containing SH2 and SH3 domains which are important for various cellular dynamic processes. Their role in cytoskeletal dynamics aligns them with many cellular mechanisms including endocytosis and cell motility.
Pathways
Nck proteins significantly influence the regulation of the actin cytoskeleton pathway and the Rho GTPases pathway. Through these pathways they interact with proteins such as WASP and Rac regulating actin polymerization and cell migration. Nck’s ability to form complex signaling networks positions it as a central actor in transducing signals that affect cell shape and movement. It acts collaboratively with multiple signaling pathways to maintain cellular plasticity and response to extracellular signals.
Specifications
Form
Liquid
Additional notes
ab97409 is purified using conventional chromatography techniques.
General info
Function
Adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling. Modulates the activation of EIF2AK2/PKR by dsRNA. May play a role in cell adhesion and migration through interaction with ephrin receptors.
Post-translational modifications
Phosphorylated on Ser and Tyr residues. Phosphorylated in response to activation of EGFR and FcERI. Phosphorylated by activated PDGFRB.
Subcellular localisation
Nucleus
Target data
Publications (1)
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Evidence-based complementary and alternative medicine : eCAM 2017:9124240 PubMed29375645
2017
Applications
Unspecified application
Species
Unspecified reactive species
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