Recombinant Human NCS1 protein is a Human Full Length protein, in the 1 to 190 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
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- Cell reports 30:3821-3836.e132020APOL1 C-Terminal Variants May Trigger Kidney Disease through Interference with APOL3 Control of Actomyosin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSophie Uzureau,Laurence Lecordier,Pierrick Uzureau,Dorle Hennig,Jonas H Graversen,Fabrice Homblé,Pepe Ekulu Mfutu,Fanny Oliveira Arcolino,Ana Raquel Ramos,Rita M La Rovere,Tomas Luyten,Marjorie Vermeersch,Patricia Tebabi,Marc Dieu,Bart Cuypers,Stijn Deborggraeve,Marion Rabant,Christophe Legendre,Søren K Moestrup,Elena Levtchenko,Geert Bultynck,Christophe Erneux,David Pérez-Morga,Etienne PaysPubMed 32187552
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M G K S N S K L K P E V V E E L T R K T Y F T E K E V Q Q W Y K G F I K D C P S G Q L D A A G F Q K I Y K Q F F P F G D P T K F A T F V F N V F D E N K D G R I E F S E F I Q A L S V T S R G T L D E K L R W A F K L Y D L D N D G Y I T R N E M L D I V D A I Y Q M V G N T V E L P E E E N T P E K R V D R I F A M M D K N A D G K L T L Q E F Q E G S K A D P S I V Q A L S L Y D G L V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Neuronal calcium sensor, regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. Directly regulates GRK1 (RHOK), but not GRK2 to GRK5. Can substitute for calmodulin (By similarity). Stimulates PI4KB kinase activity (By similarity). Involved in long-term synaptic plasticity through its interaction with PICK1 (By similarity). May also play a role in neuron differentiation through inhibition of the activity of N-type voltage-gated calcium channel (By similarity).
Alternative names
FLUP, FREQ, NCS1, Neuronal calcium sensor 1, NCS-1, Frequenin homolog, Frequenin-like protein, Frequenin-like ubiquitous protein
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Recombinant Human NCS1 protein is a Human Full Length protein, in the 1 to 190 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P62166-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 30% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M G K S N S K L K P E V V E E L T R K T Y F T E K E V Q Q W Y K G F I K D C P S G Q L D A A G F Q K I Y K Q F F P F G D P T K F A T F V F N V F D E N K D G R I E F S E F I Q A L S V T S R G T L D E K L R W A F K L Y D L D N D G Y I T R N E M L D I V D A I Y Q M V G N T V E L P E E E N T P E K R V D R I F A M M D K N A D G K L T L Q E F Q E G S K A D P S I V Q A L S L Y D G L V
- Accession
- P62166
- Protein length
- Full Length
- Predicted molecular weight
- 24.3 kDa
- Amino acids
- 1 to 190
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab183224 was purified using conventional chromatography techniques.
General info
- Function
Neuronal calcium sensor, regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. Directly regulates GRK1 (RHOK), but not GRK2 to GRK5. Can substitute for calmodulin (By similarity). Stimulates PI4KB kinase activity (By similarity). Involved in long-term synaptic plasticity through its interaction with PICK1 (By similarity). May also play a role in neuron differentiation through inhibition of the activity of N-type voltage-gated calcium channel (By similarity).
- Sequence similarities
Belongs to the recoverin family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The target 'NCS1' also known as Neuronal Calcium Sensor 1 is a small protein with a mass of approximately 22 kDa. This protein binds calcium ions and plays an important role in calcium signaling pathways. It is broadly expressed in neuronal tissues where it assists in regulating neurotransmitter release and synaptic plasticity. NCS1 belongs to a family of calcium-binding proteins characterized by EF-hand motifs which allow calcium ion attachment and subsequent conformational changes.
- Biological function summary
NCS1 modulates intracellular calcium concentrations which are critical for numerous cellular functions. It interacts with various signaling proteins and receptors impacting cell signaling and communication. NCS1 does not operate alone but functions as part of larger protein complexes which modulate its activity and extend its biological roles. Its involvement supports neuronal processes like growth and differentiation by modulating the effect of calcium signals within the nerve cells.
- Pathways
NCS1 plays a role in the phosphoinositide signaling pathway and the regulation of calcium homeostasis. It works alongside proteins such as phosphatidylinositol 4-kinase III beta (PI4KB) to maintain the balance of calcium in cells. NCS1 participation in signaling pathways ensures that calcium influx and release from intracellular stores happen efficiently which is essential for the transmission of signals across synapses.
- Associated diseases and disorders
NCS1 is associated with neurological conditions such as schizophrenia and bipolar disorder. Alterations in NCS1 expression or function can affect calcium signaling leading to disruptions in neuronal communication and function. Furthermore NCS1 interacts with dopamine receptor D2 (DRD2) which is significant in the development of these psychiatric conditions. Understanding the role of NCS1 in these disorders can provide insights into potential therapeutic targets for managing such diseases.
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SDS-PAGE - Recombinant Human NCS1 protein (ab183224)
15% SDS-PAGE analysis of ab183224 (3μg).
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