Recombinant Human NDE1 protein (Tagged) is a Human Full Length protein, in the 1 to 335 aa range, expressed in Wheat germ and suitable for SDS-PAGE.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M E D S G K T F S S E E E E A N Y W K D L A M T Y K Q R A E N T Q E E L R E F Q E G S R E Y E A E L E T Q L Q Q I E T R N R D L L S E N N R L R M E L E T I K E K F E V Q H S E G Y R Q I S A L E D D L A Q T K A I K D Q L Q K Y I R E L E Q A N D D L E R A K R A T I M S L E D F E Q R L N Q A I E R N A F L E S E L D E K E N L L E S V Q R L K D E A R D L R Q E L A V Q Q K Q E K P R T P M P S S V E A E R T D T A V Q A T G S V P S T P I A H R G P S S S L N T P G S F R R G L D D S T G G T P L T P A A R I S A L N I V G D L L R K V G A L E S K L A S C R N L V Y D Q S P N R T G G P A S G R S S K N R D G G E R R P S S T S V P L G D K G L D T S C R W L S K S T T R S S S S C
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Required for centrosome duplication and formation and function of the mitotic spindle. Essential for the development of the cerebral cortex. May regulate the production of neurons by controlling the orientation of the mitotic spindle during division of cortical neuronal progenitors of the proliferative ventricular zone of the brain. Orientation of the division plane perpendicular to the layers of the cortex gives rise to two proliferative neuronal progenitors whereas parallel orientation of the division plane yields one proliferative neuronal progenitor and a post-mitotic neuron. A premature shift towards a neuronal fate within the progenitor population may result in an overall reduction in the final number of neurons and an increase in the number of neurons in the deeper layers of the cortex.
Alternative names
NUDE, NDE1, Nuclear distribution protein nudE homolog 1, NudE
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Recombinant Human NDE1 protein (Tagged) is a Human Full Length protein, in the 1 to 335 aa range, expressed in Wheat germ and suitable for SDS-PAGE.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q9NXR1-2
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M E D S G K T F S S E E E E A N Y W K D L A M T Y K Q R A E N T Q E E L R E F Q E G S R E Y E A E L E T Q L Q Q I E T R N R D L L S E N N R L R M E L E T I K E K F E V Q H S E G Y R Q I S A L E D D L A Q T K A I K D Q L Q K Y I R E L E Q A N D D L E R A K R A T I M S L E D F E Q R L N Q A I E R N A F L E S E L D E K E N L L E S V Q R L K D E A R D L R Q E L A V Q Q K Q E K P R T P M P S S V E A E R T D T A V Q A T G S V P S T P I A H R G P S S S L N T P G S F R R G L D D S T G G T P L T P A A R I S A L N I V G D L L R K V G A L E S K L A S C R N L V Y D Q S P N R T G G P A S G R S S K N R D G G E R R P S S T S V P L G D K G L D T S C R W L S K S T T R S S S S C
- Accession
- Q9NXR1
- Protein length
- Full Length
- Predicted molecular weight
- 62.59 kDa
- Amino acids
- 1 to 335
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Required for centrosome duplication and formation and function of the mitotic spindle. Essential for the development of the cerebral cortex. May regulate the production of neurons by controlling the orientation of the mitotic spindle during division of cortical neuronal progenitors of the proliferative ventricular zone of the brain. Orientation of the division plane perpendicular to the layers of the cortex gives rise to two proliferative neuronal progenitors whereas parallel orientation of the division plane yields one proliferative neuronal progenitor and a post-mitotic neuron. A premature shift towards a neuronal fate within the progenitor population may result in an overall reduction in the final number of neurons and an increase in the number of neurons in the deeper layers of the cortex.
- Sequence similarities
Belongs to the nudE family.
- Post-translational modifications
Phosphorylated in mitosis. Phosphorylated in vitro by CDC2. Phosphorylation at Thr-246 is essential for the G2/M transition (By similarity).
- Subcellular localisation
- Cytoskeleton, Microtubule organizing center, Centrosome, Spindle
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Nucleocyte dynein axonemal 1 also known as NDE1 is an important protein involved in cell division mechanics. It weighs approximately 33 kDa. NDE1 is expressed strongly in the brain particularly in neural progenitor cells and helps in maintaining centrosome function and microtubule organization. Due to its role in centrosome attachment NDE1 plays an important role in chromosomal alignment and segregation during cell division.
- Biological function summary
NDE1 participates in centrosomal and microtubule functions. It forms a complex with other proteins such as LIS1 and dynein important for spindle positioning in dividing cells. The NDE1 complex ensures the correct orientation and symmetrical division of proliferating cells impacting tissue architecture and brain development. By partnering with these proteins NDE1 helps maintain the structural integrity of mitotic spindles.
- Pathways
NDE1 contributes significantly to the cell cycle and neurogenesis pathways. It interacts with the dynein motor complex facilitating dynein-mediated transport and positioning in dividing neurons. Within these pathways proteins such as LIS1 and DISC1 work with NDE1 indicating its broad impact on cellular positioning and neural development. NDE1 helps regulate the pathways which ensure precise cell division and brain formation.
- Associated diseases and disorders
NDE1 has links to microcephaly and lissencephaly. Mutations in NDE1 contribute to microcephaly by disrupting neuronal proliferation and migration. The protein interacts with LIS1 in this disorder which shares roles in neurodevelopment. Similarly lissencephaly characterized by smooth brain structures due to abnormal neuronal migration shows association with NDE1 dysfunction. Therefore NDE1 is significant for both normal brain development and related disorders.
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1 product image
SDS-PAGE - Recombinant Human NDE1 protein (Tagged) (ab275914)
12.5% SDS-PAGE stained with Coomassie Blue.
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