JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB275914

Recombinant Human NDE1 protein (Tagged)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human NDE1 protein (Tagged) is a Human Full Length protein, in the 1 to 335 aa range, expressed in Wheat germ, suitable for SDS-PAGE.

View Alternative Names

NUDE, NDE1, Nuclear distribution protein nudE homolog 1, NudE

1 Images
SDS-PAGE - Recombinant Human NDE1 protein (Tagged) (AB275914)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human NDE1 protein (Tagged) (AB275914)

12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q9NXR1

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MEDSGKTFSSEEEEANYWKDLAMTYKQRAENTQEELREFQEGSREYEAELETQLQQIETRNRDLLSENNRLRMELETIKEKFEVQHSEGYRQISALEDDLAQTKAIKDQLQKYIRELEQANDDLERAKRATIMSLEDFEQRLNQAIERNAFLESELDEKENLLESVQRLKDEARDLRQELAVQQKQEKPRTPMPSSVEAERTDTAVQATGSVPSTPIAHRGPSSSLNTPGSFRRGLDDSTGGTPLTPAARISALNIVGDLLRKVGALESKLASCRNLVYDQSPNRTGGPASGRSSKNRDGGERRPSSTSVPLGDKGLDTSCRWLSKSTTRSSSSC","proteinLength":"Full Length","predictedMolecularWeight":"62.59 kDa","actualMolecularWeight":null,"aminoAcidEnd":335,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q9NXR1","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nucleocyte dynein axonemal 1 also known as NDE1 is an important protein involved in cell division mechanics. It weighs approximately 33 kDa. NDE1 is expressed strongly in the brain particularly in neural progenitor cells and helps in maintaining centrosome function and microtubule organization. Due to its role in centrosome attachment NDE1 plays an important role in chromosomal alignment and segregation during cell division.
Biological function summary

NDE1 participates in centrosomal and microtubule functions. It forms a complex with other proteins such as LIS1 and dynein important for spindle positioning in dividing cells. The NDE1 complex ensures the correct orientation and symmetrical division of proliferating cells impacting tissue architecture and brain development. By partnering with these proteins NDE1 helps maintain the structural integrity of mitotic spindles.

Pathways

NDE1 contributes significantly to the cell cycle and neurogenesis pathways. It interacts with the dynein motor complex facilitating dynein-mediated transport and positioning in dividing neurons. Within these pathways proteins such as LIS1 and DISC1 work with NDE1 indicating its broad impact on cellular positioning and neural development. NDE1 helps regulate the pathways which ensure precise cell division and brain formation.

NDE1 has links to microcephaly and lissencephaly. Mutations in NDE1 contribute to microcephaly by disrupting neuronal proliferation and migration. The protein interacts with LIS1 in this disorder which shares roles in neurodevelopment. Similarly lissencephaly characterized by smooth brain structures due to abnormal neuronal migration shows association with NDE1 dysfunction. Therefore NDE1 is significant for both normal brain development and related disorders.
style
left-column

Specifications

Form

Liquid

style
left-column

General info

Function

Required for centrosome duplication and formation and function of the mitotic spindle. Essential for the development of the cerebral cortex. May regulate the production of neurons by controlling the orientation of the mitotic spindle during division of cortical neuronal progenitors of the proliferative ventricular zone of the brain. Orientation of the division plane perpendicular to the layers of the cortex gives rise to two proliferative neuronal progenitors whereas parallel orientation of the division plane yields one proliferative neuronal progenitor and a post-mitotic neuron. A premature shift towards a neuronal fate within the progenitor population may result in an overall reduction in the final number of neurons and an increase in the number of neurons in the deeper layers of the cortex.

Sequence similarities

Belongs to the nudE family.

Post-translational modifications

Phosphorylated in mitosis. Phosphorylated in vitro by CDC2. Phosphorylation at Thr-246 is essential for the G2/M transition (By similarity).

Subcellular localisation

Cytoskeleton

style
left-column

Product protocols

style
left-column

Target data

Required for centrosome duplication and formation and function of the mitotic spindle. Essential for the development of the cerebral cortex. May regulate the production of neurons by controlling the orientation of the mitotic spindle during division of cortical neuronal progenitors of the proliferative ventricular zone of the brain. Orientation of the division plane perpendicular to the layers of the cortex gives rise to two proliferative neuronal progenitors whereas parallel orientation of the division plane yields one proliferative neuronal progenitor and a post-mitotic neuron. A premature shift towards a neuronal fate within the progenitor population may result in an overall reduction in the final number of neurons and an increase in the number of neurons in the deeper layers of the cortex.
See full target information NDE1
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB313415

Anti-NDE1 Antibody [RP23040189]

Recombinant

Western blot - Anti-NDE1 Antibody [RP23040189] (AB313415)

  • 0
  • 0
Proteins & Peptides

AB114584

Recombinant Human DPD protein

SDS-PAGE - Recombinant Human DPD protein (AB114584)

  • 0
  • 0
Proteins & Peptides

AB219276

Recombinant human PGAM2 protein (Active)

SDS-PAGE - Recombinant human PGAM2 protein (Active) (AB219276)

  • 0
  • 0
Proteins & Peptides

AB156351

Recombinant Human Ephrin A3 protein (denatured)

SDS-PAGE - Recombinant Human Ephrin A3 protein (denatured) (AB156351)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com