Recombinant Human NDEL1 protein is a Human Full Length protein, in the 1 to 328 aa range, expressed in Escherichia coli, with >80% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >80% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M D G E D I P D F S S L K E E T A Y W K E L S L K Y K Q S F Q E A R D E L V E F Q E G S R E L E A E L E A Q L V Q A E Q R N R D L Q A D N Q R L K Y E V E A L K E K L E H Q Y A Q S Y K Q V S V L E D D L S Q T R A I K E Q L H K Y V R E L E Q A N D D L E R A K R A T I V S L E D F E Q R L N Q A I E R N A F L E S E L D E K E S L L V S V Q R L K D E A R D L R Q E L A V R E R Q Q E V T R K S A P S S P T L D C E K M D S A V Q A S L S L P A T P V G K G T E N T F P S P K A I P N G F G T S P L T P S A R I S A L N I V G D L L R K V G A L E S K L A A C R N F A K D Q A S R K S Y I S G N V N C G V L N G N G T K F S R S G H T S F F D K G Q E K V I F P T L F M G Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Required for organization of the cellular microtubule array and microtubule anchoring at the centrosome. May regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome. Also positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the centripetal motion of secretory vesicles and the coupling of the nucleus and centrosome. Also required during brain development for the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Plays a role, together with DISC1, in the regulation of neurite outgrowth. Required for mitosis in some cell types but appears to be dispensible for mitosis in cortical neuronal progenitors, which instead requires NDE1. Facilitates the polymerization of neurofilaments from the individual subunits NEFH and NEFL. Positively regulates lysosome peripheral distribution and ruffled border formation in osteoclasts (By similarity).
Alternative names
EOPA, MITAP1, NUDEL, NDEL1, Nuclear distribution protein nudE-like 1, Protein Nudel, Mitosin-associated protein 1
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Recombinant Human NDEL1 protein is a Human Full Length protein, in the 1 to 328 aa range, expressed in Escherichia coli, with >80% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >80% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q9GZM8-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M D G E D I P D F S S L K E E T A Y W K E L S L K Y K Q S F Q E A R D E L V E F Q E G S R E L E A E L E A Q L V Q A E Q R N R D L Q A D N Q R L K Y E V E A L K E K L E H Q Y A Q S Y K Q V S V L E D D L S Q T R A I K E Q L H K Y V R E L E Q A N D D L E R A K R A T I V S L E D F E Q R L N Q A I E R N A F L E S E L D E K E S L L V S V Q R L K D E A R D L R Q E L A V R E R Q Q E V T R K S A P S S P T L D C E K M D S A V Q A S L S L P A T P V G K G T E N T F P S P K A I P N G F G T S P L T P S A R I S A L N I V G D L L R K V G A L E S K L A A C R N F A K D Q A S R K S Y I S G N V N C G V L N G N G T K F S R S G H T S F F D K G Q E K V I F P T L F M G Q
- Accession
- Q9GZM8
- Protein length
- Full Length
- Predicted molecular weight
- 39 kDa
- Amino acids
- 1 to 328
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Required for organization of the cellular microtubule array and microtubule anchoring at the centrosome. May regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome. Also positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the centripetal motion of secretory vesicles and the coupling of the nucleus and centrosome. Also required during brain development for the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Plays a role, together with DISC1, in the regulation of neurite outgrowth. Required for mitosis in some cell types but appears to be dispensible for mitosis in cortical neuronal progenitors, which instead requires NDE1. Facilitates the polymerization of neurofilaments from the individual subunits NEFH and NEFL. Positively regulates lysosome peripheral distribution and ruffled border formation in osteoclasts (By similarity).
- Sequence similarities
Belongs to the nudE family.
- Post-translational modifications
Phosphorylated in mitosis. Can be phosphorylated by CDK1, CDK5 and MAPK1. Phosphorylation by CDK5 promotes interaction with KATNA1 and YWHAE.
- Subcellular localisation
- Cytoskeleton, Microtubule organizing center, Centrosome, Spindle
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as Nudel
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NDEL1 also known as Nudel or nuclear distribution element-like 1 is a protein with a molecular mass of around 38 kDa. It plays a role mechanically by participating in microtubule and centrosome-related processes. In terms of expression NDEL1 is found in both the central nervous system and several peripheral tissues. Its localization relates to its function in cellular dynamics particularly involving the cytoskeleton.
- Biological function summary
NDEL1 interacts within a multi-protein complex called the dynein motor complex. This association is critical for the process of neuronal migration and axonal transport involving the movement of cellular components along microtubules. The protein acts as a linking agent facilitating microtubule assembly and organization which are essential for maintaining cellular structure and function.
- Pathways
The role of NDEL1 in cytoskeletal dynamics integrates into key pathways such as the cell cycle and neurodevelopmental processes. It interacts with proteins like LIS1 in the cell cycle pathway which affects mitosis. Moreover its interaction with dynein emphasizes its role in intracellular transport during neurodevelopment indicating its aosphysical involvement in brain architecture.
- Associated diseases and disorders
NDEL1 has a significant connection with neurodevelopmental disorders and neurodegenerative diseases. Its association with LIS1 and the disruption of their interaction contributes to lissencephaly a condition marked by smooth brain surface. Additionally alterations in NDEL1 function also connect with Alzheimer's disease due to its role in neuronal structural maintenance alongside proteins such as tau that become dysregulated.
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1 product image
SDS-PAGE - Recombinant Human NDEL1 protein (ab183232)
15% SDS-PAGE analysis of ab183232 (3μg)
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