JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB183232

Recombinant Human NDEL1 protein (His tag N-Terminus)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human NDEL1 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 328 aa range, expressed in Escherichia coli, with >80%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

EOPA, MITAP1, NUDEL, NDEL1, Nuclear distribution protein nudE-like 1, Protein Nudel, Mitosin-associated protein 1

1 Images
SDS-PAGE - Recombinant Human NDEL1 protein (His tag N-Terminus) (AB183232)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human NDEL1 protein (His tag N-Terminus) (AB183232)

15% SDS-PAGE analysis of ab183232 (3μg)

Key facts

Purity

>80% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q9GZM8

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

This product was previously labelled as Nudel
style
left-column

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMDGEDIPDFSSLKEETAYWKELSLKYKQSFQEARDELVEFQEGSRELEAELEAQLVQAEQRNRDLQADNQRLKYEVEALKEKLEHQYAQSYKQVSVLEDDLSQTRAIKEQLHKYVRELEQANDDLERAKRATIVSLEDFEQRLNQAIERNAFLESELDEKESLLVSVQRLKDEARDLRQELAVRERQQEVTRKSAPSSPTLDCEKMDSAVQASLSLPATPVGKGTENTFPSPKAIPNGFGTSPLTPSARISALNIVGDLLRKVGALESKLAACRNFAKDQASRKSYISGNVNCGVLNGNGTKFSRSGHTSFFDKGQEKVIFPTLFMGQ","proteinLength":"Full Length","predictedMolecularWeight":"39 kDa","actualMolecularWeight":null,"aminoAcidEnd":328,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9GZM8","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NDEL1 also known as Nudel or nuclear distribution element-like 1 is a protein with a molecular mass of around 38 kDa. It plays a role mechanically by participating in microtubule and centrosome-related processes. In terms of expression NDEL1 is found in both the central nervous system and several peripheral tissues. Its localization relates to its function in cellular dynamics particularly involving the cytoskeleton.
Biological function summary

NDEL1 interacts within a multi-protein complex called the dynein motor complex. This association is critical for the process of neuronal migration and axonal transport involving the movement of cellular components along microtubules. The protein acts as a linking agent facilitating microtubule assembly and organization which are essential for maintaining cellular structure and function.

Pathways

The role of NDEL1 in cytoskeletal dynamics integrates into key pathways such as the cell cycle and neurodevelopmental processes. It interacts with proteins like LIS1 in the cell cycle pathway which affects mitosis. Moreover its interaction with dynein emphasizes its role in intracellular transport during neurodevelopment indicating its aosphysical involvement in brain architecture.

NDEL1 has a significant connection with neurodevelopmental disorders and neurodegenerative diseases. Its association with LIS1 and the disruption of their interaction contributes to lissencephaly a condition marked by smooth brain surface. Additionally alterations in NDEL1 function also connect with Alzheimer's disease due to its role in neuronal structural maintenance alongside proteins such as tau that become dysregulated.
style
left-column

Specifications

Form

Liquid

style
left-column

General info

Function

Required for organization of the cellular microtubule array and microtubule anchoring at the centrosome. May regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome. Also positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the centripetal motion of secretory vesicles and the coupling of the nucleus and centrosome. Also required during brain development for the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Plays a role, together with DISC1, in the regulation of neurite outgrowth. Required for mitosis in some cell types but appears to be dispensible for mitosis in cortical neuronal progenitors, which instead requires NDE1. Facilitates the polymerization of neurofilaments from the individual subunits NEFH and NEFL. Positively regulates lysosome peripheral distribution and ruffled border formation in osteoclasts (By similarity).

Sequence similarities

Belongs to the nudE family.

Post-translational modifications

Phosphorylated in mitosis. Can be phosphorylated by CDK1, CDK5 and MAPK1. Phosphorylation by CDK5 promotes interaction with KATNA1 and YWHAE.. Palmitoylation at Cys-273 reduces affinity for dynein.

Subcellular localisation

Cytoskeleton

style
left-column

Product protocols

style
left-column

Target data

Required for organization of the cellular microtubule array and microtubule anchoring at the centrosome. May regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome. Also positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the centripetal motion of secretory vesicles and the coupling of the nucleus and centrosome. Also required during brain development for the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Plays a role, together with DISC1, in the regulation of neurite outgrowth. Required for mitosis in some cell types but appears to be dispensible for mitosis in cortical neuronal progenitors, which instead requires NDE1. Facilitates the polymerization of neurofilaments from the individual subunits NEFH and NEFL. Positively regulates lysosome peripheral distribution and ruffled border formation in osteoclasts (By similarity).
See full target information NDEL1
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB124895

Anti-NDEL1 antibody [EPR5068]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDEL1 antibody [EPR5068] (AB124895)

  • 0
  • 0
Proteins & Peptides

AB116188

Recombinant Human GAS41 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human GAS41 protein (His tag N-Terminus) (AB116188)

  • 0
  • 0
Proteins & Peptides

AB152790

Recombinant Human FIP protein

SDS-PAGE - Recombinant Human FIP protein (AB152790)

  • 0
  • 0
Proteins & Peptides

AB113126

Recombinant Human TNNC1 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human TNNC1 protein (His tag N-Terminus) (AB113126)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com