Recombinant Human NDST3 protein is a Human Fragment protein, in the 162 to 259 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
I G F H K T S E K S V Q S F Q L K G F P F S I Y G N L A V K D C C I N P H S P L I R V T K S S K L E K G S L P G T D W T V F Q I N H S A Y Q P V I F A K V K T P E N L S P S I S K G A F Y A T I I H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Has high deacetylase activity but low sulfotransferase activity.
Alternative names
HSST3, UNQ2544/PRO4998, NDST3, Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 3, Glucosaminyl N-deacetylase/N-sulfotransferase 3, N-heparan sulfate sulfotransferase 3, NDST-3, hNDST-3, N-HSST 3
Recommended products
Recombinant Human NDST3 protein is a Human Fragment protein, in the 162 to 259 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- O95803-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- I G F H K T S E K S V Q S F Q L K G F P F S I Y G N L A V K D C C I N P H S P L I R V T K S S K L E K G S L P G T D W T V F Q I N H S A Y Q P V I F A K V K T P E N L S P S I S K G A F Y A T I I H
- Accession
- O95803
- Protein length
- Fragment
- Amino acids
- 162 to 259
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Has high deacetylase activity but low sulfotransferase activity.
- Sequence similarities
Belongs to the sulfotransferase 1 family. NDST subfamily.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NDST3 also known as N-deacetylase/N-sulfotransferase 3 is an enzyme that facilitates important steps in the biosynthesis of heparan sulfate by removing N-acetyl groups and adding sulfate groups to glucosamine residues. This enzyme has a molecular mass of approximately 110 kDa and is primarily expressed in tissues with high proteoglycan content such as the brain. Its activity is critical for modifying heparan sulfate chains which serves a role in regulating various biological processes.
- Biological function summary
NDST3 engages in the modification of heparan sulfate which is an important component of the extracellular matrix. NDST3 is involved in a complex that includes various other NDST family members each contributing distinct functions in heparan sulfate biosynthesis. Through its enzymatic activity NDST3 influences cell signaling and interaction dynamics by producing the variably sulfated domains in heparan sulfate chains that determine ligand binding specificity.
- Pathways
NDST3's function in heparan sulfate modification is important in the fibroblast growth factor (FGF) signaling pathway and the Wnt signaling pathway. In these pathways the modified heparan sulfate participates in the regulation of important ligands and receptors which is necessary for proper signal transduction. NDST3 works in concert with proteins such as fibroblast growth factor receptor (FGFR) and Wnt receptors influencing cellular responses and developmental processes.
- Associated diseases and disorders
Defects in NDST3 activity have been implicated in neurodevelopmental disorders and cancer. Altered NDST3 function impacts important signaling pathways in the nervous system and influences tumor progression by affecting cell communication and migration. The enzyme’s relationship with signaling proteins such as FGFR connects its dysfunction to the progression of conditions highlighting its importance in maintaining cellular homeostasis and integrity in human health.
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1 product image
SDS-PAGE - Recombinant Human NDST3 protein (ab160446)
ab160446 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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