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AB134598

Recombinant Human NDUFAF1 protein

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Recombinant Human NDUFAF1 protein is a Human Full Length protein, in the 25 to 327 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

CIA30, CGI-65, NDUFAF1, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 1

1 Images
SDS-PAGE - Recombinant Human NDUFAF1 protein (AB134598)
  • SDS-PAGE

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SDS-PAGE - Recombinant Human NDUFAF1 protein (AB134598)

15% SDS-PAGE analysis of ab134598 (3Μg).

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q9Y375

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 40% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.06% EDTA, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSYPFLGIRFAEYSSSLQKPVASPGKASSQRKTEGDLQGDHQKEVALDITSSEEKPDVSFDKAIRDEAIYHFRLLKDEIVDHWRGPEGHPLHEVLLEQAKVVWQFRGKEDLDKWTVTSDKTIGGRSEVFLKMGKNNQSALLYGTLSSEAPQDGESTRSGYCAMISRIPRGAFERKMSYDWSQFNTLYLRVRGDGRPWMVNIKEDTDFFQRTNQMYSYFMFTRGGPYWQEVKIPFSKFFFSNRGRIRDVQHELPLDKISSIGFTLADKVDGPFFLEIDFIGVFTDPAHTEEFAYENSPELNPRLFK","proteinLength":"Full Length","predictedMolecularWeight":"37 kDa","actualMolecularWeight":null,"aminoAcidEnd":327,"aminoAcidStart":25,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9Y375","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NDUFAF1 also known as NADH dehydrogenase (ubiquinone) complex I assembly factor 1 plays an essential role in the mitochondrial respiratory chain by assisting the assembly of complex I. This protein has a mass of approximately 36 kDa. NDUFAF1 expression occurs mainly in tissues with high metabolic activity like the brain heart and skeletal muscle. It facilitates the proper formation and function of the complex I assembly allowing efficient electron transport essential for cellular respiration.
Biological function summary

NDUFAF1 is a critical assembly factor integral to forming the mitochondrial complex I also known as NADH:ubiquinone oxidoreductase. NDUFAF1 contributes to the stability and proper function of this multi-component enzyme system which is important in cellular energy production. As part of the complex it ensures efficient ATP generation through oxidative phosphorylation which is the primary source of energy in aerobic eukaryotic cells.

Pathways

NDUFAF1 functions predominantly in the oxidative phosphorylation pathway. This involvement connects NDUFAF1 to the fundamental molecular processes sustaining life. The protein plays a role alongside others like NDUFV1 and NDUFS1 in maintaining complex I activity. These relationships ensure the effective transfer of electrons from NADH to ubiquinone promoting efficient energy production throughout the cell.

NDUFAF1 mutations or dysfunction have been linked to mitochondrial disorders such as Leigh syndrome and complex I deficiency. These conditions are often characterized by a disruption in mitochondrial energy metabolism leading to severe neurological and muscular symptoms. Furthermore NDUFAF1 dysfunction can indirectly affect other proteins like NDUFS4 which is also involved in complex I assembly and function exacerbating the disease phenotypes. Mitochondrial diseases often arise from impaired pathways involving several network proteins highlighting the significance of proper NDUFAF1 function.
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Specifications

Form

Liquid

Additional notes

ab134598 was purified using conventional chromatography techniques.

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General info

Function

As part of the MCIA complex, involved in the assembly of the mitochondrial complex I.

Sequence similarities

Belongs to the CIA30 family.

Subcellular localisation

Mitochondrion

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Product protocols

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Target data

As part of the MCIA complex, involved in the assembly of the mitochondrial complex I.
See full target information NDUFAF1
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