Recombinant Human NDUFAF4 protein is a Human Full Length protein, in the 1 to 175 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M G A L V I R G I R N F N L E N R A E R E I S K M K P S V A P R H P S T N S L L R E Q I S L Y P E V K G E I A R K D E K L L S F L K D V Y V D S K D P V S S L Q V K A A E T C Q E P K E F R L P K D H H F D M I N I K S I P K G K I S I V E A L T L L N N H K L F P E T W T A E K I M Q E Y Q L E Q K D V N S L L K Y F V T F E V E I F P P E D K K A I R S K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Involved in the assembly of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I) (PubMed:18179882, PubMed:28853723). May be involved in cell proliferation and survival of hormone-dependent tumor cells. May be a regulator of breast tumor cell invasion.
Alternative names
C6orf66, HRPAP20, HSPC125, My013, NDUFAF4, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4, Hormone-regulated proliferation-associated protein of 20 kDa
Recommended products
Recombinant Human NDUFAF4 protein is a Human Full Length protein, in the 1 to 175 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q9P032-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M G A L V I R G I R N F N L E N R A E R E I S K M K P S V A P R H P S T N S L L R E Q I S L Y P E V K G E I A R K D E K L L S F L K D V Y V D S K D P V S S L Q V K A A E T C Q E P K E F R L P K D H H F D M I N I K S I P K G K I S I V E A L T L L N N H K L F P E T W T A E K I M Q E Y Q L E Q K D V N S L L K Y F V T F E V E I F P P E D K K A I R S K
- Accession
- Q9P032
- Protein length
- Full Length
- Predicted molecular weight
- 22.7 kDa
- Amino acids
- 1 to 175
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab134610 was purified using conventional chromatography techniques.
General info
- Function
Involved in the assembly of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I) (PubMed:18179882, PubMed:28853723). May be involved in cell proliferation and survival of hormone-dependent tumor cells. May be a regulator of breast tumor cell invasion.
- Sequence similarities
Belongs to the NDUFAF4 family.
- Post-translational modifications
Phosphorylated on serine. Prolactin stimulate serine phosphorylation (By similarity).
- Subcellular localisation
- Mitochondrion
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NDUFAF4 also known as Complex I Assembly Factor 2 plays an integral role in the assembly of NADH:ubiquinone oxidoreductase complex I within mitochondria. It has a mass of approximately 20 kilodaltons. This protein is widely expressed in various tissues with heightened levels found in organs with high energy demands such as the brain and heart. Functionally NDUFAF4 mediates the proper formation and stabilization of the mitochondrial complex I by contributing to the assembly of its subunits.
- Biological function summary
NDUFAF4 participates in the mitochondrial electron transport chain as part of the complex I assembly. It acts as an assembly factor for complex I the first component of the chain which is important for initiating electron transport from NADH to the rest of the chain. This complex contains multiple subunits that require precise assembly and NDUFAF4 ensures the correct incorporation of these subunits. Its role is essential for maintaining efficient mitochondrial respiration thereby supporting ATP production.
- Pathways
The NDUFAF4 protein ensures proper electron transport chain function by facilitating complex I assembly. It interacts within mitochondrial pathways involving oxidative phosphorylation and reactive oxygen species (ROS) regulation. It associates with other proteins such as NDUFS3 and NDUFS4 as part of the complex I formation process. Progressive assembly of complex I facilitated by NDUFAF4 is an important step in maintaining cellular energy metabolism.
- Associated diseases and disorders
Improper function or expression of NDUFAF4 leads to mitochondrial disorders including Leigh syndrome and other metabolic encephalopathies. Mutations in NDUFAF4 disrupt complex I assembly resulting in impaired mitochondrial respiration and energy production. This protein also relates to Parkinson’s disease where its involvement in complex I assembly connects it to proteins like Parkin which are associated with mitochondrial dysfunction in dopaminergic neurons.
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1 product image
SDS-PAGE - Recombinant Human NDUFAF4 protein (ab134610)
15% SDS-PAGE analysis of ab134610 (3μg).
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