Recombinant Human NDUFB8 protein is a Human Full Length protein, in the 1 to 186 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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- Cancer cell 35:721-737.e92019Mitochondrial ClpP-Mediated Proteolysis Induces Selective Cancer Cell Lethality.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJo Ishizawa et. al.PubMed 31056398
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M A V A R A G V L G V Q W L Q R A S R N V M P L G A R T A S H M T K D M F P G P Y P R T P E E R A A A A K K Y N M R V E D Y E P Y P D D G M G Y G D Y P K L P D R S Q H E R D P W Y S W D Q P G L R L N W G E P M H W H L D M Y N R N R V D T S P T P V S W H V M C M Q L F G F L A F M I F M C W V G D V Y P V Y Q P V G P K Q Y P Y N N L Y L E R G G D P S K E P E R V V H Y E I
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Alternative names
Complex I-ASHI, NADH-ubiquinone oxidoreductase ASHI subunit, CI-ASHI, NDUFB8
Recommended products
Recombinant Human NDUFB8 protein is a Human Full Length protein, in the 1 to 186 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- O95169-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M A V A R A G V L G V Q W L Q R A S R N V M P L G A R T A S H M T K D M F P G P Y P R T P E E R A A A A K K Y N M R V E D Y E P Y P D D G M G Y G D Y P K L P D R S Q H E R D P W Y S W D Q P G L R L N W G E P M H W H L D M Y N R N R V D T S P T P V S W H V M C M Q L F G F L A F M I F M C W V G D V Y P V Y Q P V G P K Q Y P Y N N L Y L E R G G D P S K E P E R V V H Y E I
- Accession
- O95169
- Protein length
- Full Length
- Predicted molecular weight
- 46.2 kDa
- Amino acids
- 1 to 186
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
- Sequence similarities
Belongs to the complex I NDUFB8 subunit family.
- Subcellular localisation
- Mitochondrion inner membrane
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The NDUFB8 protein also known as NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 is an integral component of the mitochondrial inner membrane. It is a part of the electron transport chain and plays a role in mitochondrial oxidative phosphorylation. NDUFB8 has a molecular weight of approximately 19 kDa. It is broadly expressed in tissues with high-energy demands like the heart brain skeletal muscle and liver. This expression indicates its significant involvement in energy metabolism.
- Biological function summary
NDUFB8 is an accessory subunit of the mitochondrial Complex I the largest enzyme of the respiratory chain. It assists in the transfer of electrons from NADH to ubiquinone a critical step in cellular respiration that contributes to ATP production. Though not directly involved in catalysis its presence is essential for the structural integrity and assembly of Complex I. By maintaining this complex's structure NDUFB8 helps ensure efficient energy production in cells.
- Pathways
NDUFB8 participates in the oxidative phosphorylation pathway playing a significant role in the mitochondrial respiratory chain. It influences the production of ATP the primary energy carrier in cells. Its association with Complex I links it to other subunits and proteins like NDUFA9 and NDUFA1 in the same pathway. These connections emphasize NDUFB8's importance within the pathway and its contribution to cellular energy management.
- Associated diseases and disorders
Dysfunction in NDUFB8 is linked to mitochondrial disorders often resulting in neurological diseases or muscle weakness such as Leigh syndrome. Mutations affecting NDUFB8 or its associated proteins like NDUFA13 could disrupt the assembly or function of Complex I leading to impaired energy production. This connection highlights the role of NDUFB8 in maintaining cellular energy balance and its impact on health when malfunctioning.
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1 product image
SDS-PAGE - Recombinant Human NDUFB8 protein (ab152558)
12.5% SDS-PAGE analysis of ab152558 stained with Coomassie Blue.
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