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AB174413

Recombinant Human NDUFS2 protein (denatured) (His tag N-Terminus)

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Recombinant Human NDUFS2 protein (denatured) (His tag N-Terminus) is a Human Fragment protein, in the 77 to 463 aa range, expressed in Escherichia coli, with >80%, suitable for SDS-PAGE.

View Alternative Names

Complex I-49kD, NADH-ubiquinone oxidoreductase 49 kDa subunit, CI-49kD, NDUFS2

1 Images
SDS-PAGE - Recombinant Human NDUFS2 protein (denatured) (His tag N-Terminus) (AB174413)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human NDUFS2 protein (denatured) (His tag N-Terminus) (AB174413)

15% SDS-PAGE analysis of ab174413 (3 μg).

Key facts

Purity

>80% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

O75306

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSVKNITLNFGPQHPAAHGVLRLVMELSGEMVRKCDPHIGLLHRGTEKLIEYKTYLQALPYFDRLDYVSMMCNEQAYSLAVEKLLNIRPPPRAQWIRVLFGEITRLLNHIMAVTTHALDLGAMTPFFWLFEEREKMFEFYERVSGARMHAAYIRPGGVHQDLPLGLMDDIYQFSKNFSLRLDELEELLTNNRIWRNRTIDIGVVTAEEALNYGFSGVMLRGSGIQWDLRKTQPYDVYDQVEFDVPVGSRGDCYDRYLCRVEEMRQSLRIIAQCLNKMPPGEIKVDDAKVSPPKRAEMKTSMESLIHHFKLYTEGYQVPPGATYTAIEAPKGEFGVYLVSDGSSRPYRCKIKAPGFAHLAGLDKMSKGHMLADVVAIIGTQDIVFGEVDR","proteinLength":"Fragment","predictedMolecularWeight":"46.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":463,"aminoAcidStart":77,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O75306","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NDUFS2 also known as NADH dehydrogenase [ubiquinone] iron-sulfur protein 2 is a significant component of complex I in the mitochondrial respiratory chain. This protein has a mass of approximately 54 kDa and is primarily found in mitochondrial membranes of cells across various tissues. It plays an essential role in electron transfer working closely with other subunits to facilitate the conversion of NADH to NAD+ contributing to the process of oxidative phosphorylation.
Biological function summary

NDUFS2 serves an important role in energy production as part of the multi-subunit complex I which is also known as NADH:ubiquinone oxidoreductase. This complex catalyzes the first step in the mitochondrial electron transport chain transferring electrons from NADH to ubiquinone. Through this process NDUFS2 helps power ATP synthesis by contributing to the establishment of a proton gradient across the inner mitochondrial membrane which is fundamental to cellular energy metabolism.

Pathways

The function of NDUFS2 is integral to both the oxidative phosphorylation and citric acid cycle pathways. Within oxidative phosphorylation NDUFS2 associates closely with other complex I subunits such as NDUFS1 and NDUFV1. These associations allow it to effectively participate in the electron transport chain facilitating energy production. The citric acid cycle indirectly impacts NDUFS2's function by supplying NADH which is necessary for complex I activity and efficient energy conversion.

NDUFS2 mutations and dysfunctions relate to disorders such as Leigh syndrome and some mitochondrial myopathies. Leigh syndrome a severe neurological disorder often involves deficiencies in complex I where NDUFS2 plays a significant role. This protein also shows connections to other mitochondrial proteins like NDUFA12 and NDUFB6 which when also impaired can contribute to disease manifestations. The study and understanding of NDUFS2's role in these conditions are important for developing potential therapeutic strategies.
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Specifications

Form

Liquid

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General info

Function

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (PubMed : 22036843, PubMed : 28031252, PubMed : 30922174). Essential for the catalytic activity of complex I (PubMed : 22036843, PubMed : 30922174). Essential for the assembly of complex I (By similarity). Redox-sensitive, critical component of the oxygen-sensing pathway in the pulmonary vasculature which plays a key role in acute pulmonary oxygen-sensing and hypoxic pulmonary vasoconstriction (PubMed : 30922174). Plays an important role in carotid body sensing of hypoxia (By similarity). Essential for glia-like neural stem and progenitor cell proliferation, differentiation and subsequent oligodendrocyte or neuronal maturation (By similarity).

Sequence similarities

Belongs to the complex I 49 kDa subunit family.

Post-translational modifications

Dimethylation at Arg-118 by NDUFAF7 takes place after NDUFS2 assembles into the complex I, leading to stabilize the early intermediate complex (PubMed:24089531, PubMed:24838397).

Subcellular localisation

Mitochondrion inner membrane

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Product protocols

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Target data

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (PubMed : 22036843, PubMed : 28031252, PubMed : 30922174). Essential for the catalytic activity of complex I (PubMed : 22036843, PubMed : 30922174). Essential for the assembly of complex I (By similarity). Redox-sensitive, critical component of the oxygen-sensing pathway in the pulmonary vasculature which plays a key role in acute pulmonary oxygen-sensing and hypoxic pulmonary vasoconstriction (PubMed : 30922174). Plays an important role in carotid body sensing of hypoxia (By similarity). Essential for glia-like neural stem and progenitor cell proliferation, differentiation and subsequent oligodendrocyte or neuronal maturation (By similarity).
See full target information NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial
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Complementary Products

Primary Antibodies

AB110249

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Primary Antibodies

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BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

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