Recombinant Human NDUFV2 protein is a Human Full Length protein, in the 33 to 249 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, MS
- Biologically active
No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S G A G G A L F V H R D T P E N N P D T P F D F T P E N Y K R I E A I V K N Y P E G H K A A A V L P V L D L A Q R Q N G W L P I S A M N K V A E V L Q V P P M R V Y E V A T F Y T M Y N R K P V G K Y H I Q V C T T T P C M L R N S D S I L E A I Q K K L G I K V G E T T P D K L F T L I E V E C L G A C V N A P M V Q I N D N Y Y E D L T A K D I E E I I D E L K A G K I P K P G P R S G R F S C E P A G G L T S L T E P P K G P G F G V Q A G L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor.
Alternative names
NADH-ubiquinone oxidoreductase 24 kDa subunit, NDUFV2
Recommended products
Recombinant Human NDUFV2 protein is a Human Full Length protein, in the 33 to 249 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Alternative names
NADH-ubiquinone oxidoreductase 24 kDa subunit, NDUFV2
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, MS
- Biologically active
No
- Accession
- P19404-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S G A G G A L F V H R D T P E N N P D T P F D F T P E N Y K R I E A I V K N Y P E G H K A A A V L P V L D L A Q R Q N G W L P I S A M N K V A E V L Q V P P M R V Y E V A T F Y T M Y N R K P V G K Y H I Q V C T T T P C M L R N S D S I L E A I Q K K L G I K V G E T T P D K L F T L I E V E C L G A C V N A P M V Q I N D N Y Y E D L T A K D I E E I I D E L K A G K I P K P G P R S G R F S C E P A G G L T S L T E P P K G P G F G V Q A G L
- Accession
- Protein length
Full Length
- Predicted molecular weight
26.1 kDa
- Amino acids
33 to 249
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
- Additional notes
ab171712 was purified by anion-exchange and gel-filtration chromatography techniques.
General info
- Function
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor.
- Sequence similarities
Belongs to the complex I 24 kDa subunit family.
- Subcellular localisation
Mitochondrion inner membrane
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NDUFV2 also known as NADH dehydrogenase [ubiquinone] flavoprotein 2 functions as a core subunit of complex I in the mitochondrial electron transport chain. This protein has a mass of approximately 24 kDa and plays a pivotal role in electron transfer by linking flavin mononucleotide and iron-sulfur centers. It is expressed in various tissues but shows high levels in cells with elevated energy demands such as muscle and brain tissues.
- Biological function summary
NDUFV2 contributes to energy production in the cell by facilitating the transfer of electrons from NADH to the ubiquinone pool. As part of complex I which is the first enzyme complex in the respiratory electron transport chain it helps in establishing the proton gradient across the inner mitochondrial membrane. The activity of NDUFV2 is essential for ATP synthesis and its proper function ensures efficient cellular respiration.
- Pathways
NDUFV2 operates in the oxidative phosphorylation pathway an important energy production route. This protein is intricately linked with other complex I subunits including NDUFS1 which work together to initiate the process of oxidative phosphorylation. Another key component in this pathway is ubiquinone which serves as the electron transfer intermediary continuing the electron passage through the chain.
- Associated diseases and disorders
Mutations in NDUFV2 have associations with neurodegenerative diseases and cardiac dysfunction. For instance patients with Leber Hereditary Optic Neuropathy often present alterations in complex I that involve NDUFV2. Additionally disruptions in its function can influence the onset of conditions such as cardiomyopathy where complex I perturbation including proteins like NDUFS1 can compromise mitochondrial energy production leading to heart muscle inefficiency.
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1 product image
SDS-PAGE - Recombinant Human NDUFV2 protein (ab171712)
15% SDS-PAGE analysis of ab171712 (3μg).
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