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AB171712

Recombinant Human NDUFV2 protein

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Recombinant Human NDUFV2 protein is a Human Full Length protein, in the 33 to 249 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

NDUFV2, NADH-ubiquinone oxidoreductase 24 kDa subunit

1 Images
SDS-PAGE - Recombinant Human NDUFV2 protein (AB171712)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human NDUFV2 protein (AB171712)

15% SDS-PAGE analysis of ab171712 (3μg).

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

P19404

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSGAGGALFVHRDTPENNPDTPFDFTPENYKRIEAIVKNYPEGHKAAAVLPVLDLAQRQNGWLPISAMNKVAEVLQVPPMRVYEVATFYTMYNRKPVGKYHIQVCTTTPCMLRNSDSILEAIQKKLGIKVGETTPDKLFTLIEVECLGACVNAPMVQINDNYYEDLTAKDIEEIIDELKAGKIPKPGPRSGRFSCEPAGGLTSLTEPPKGPGFGVQAGL","proteinLength":"Full Length","predictedMolecularWeight":"26.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":249,"aminoAcidStart":33,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P19404","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NDUFV2 also known as NADH dehydrogenase [ubiquinone] flavoprotein 2 functions as a core subunit of complex I in the mitochondrial electron transport chain. This protein has a mass of approximately 24 kDa and plays a pivotal role in electron transfer by linking flavin mononucleotide and iron-sulfur centers. It is expressed in various tissues but shows high levels in cells with elevated energy demands such as muscle and brain tissues.
Biological function summary

NDUFV2 contributes to energy production in the cell by facilitating the transfer of electrons from NADH to the ubiquinone pool. As part of complex I which is the first enzyme complex in the respiratory electron transport chain it helps in establishing the proton gradient across the inner mitochondrial membrane. The activity of NDUFV2 is essential for ATP synthesis and its proper function ensures efficient cellular respiration.

Pathways

NDUFV2 operates in the oxidative phosphorylation pathway an important energy production route. This protein is intricately linked with other complex I subunits including NDUFS1 which work together to initiate the process of oxidative phosphorylation. Another key component in this pathway is ubiquinone which serves as the electron transfer intermediary continuing the electron passage through the chain.

Mutations in NDUFV2 have associations with neurodegenerative diseases and cardiac dysfunction. For instance patients with Leber Hereditary Optic Neuropathy often present alterations in complex I that involve NDUFV2. Additionally disruptions in its function can influence the onset of conditions such as cardiomyopathy where complex I perturbation including proteins like NDUFS1 can compromise mitochondrial energy production leading to heart muscle inefficiency.
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Specifications

Form

Liquid

Additional notes

ab171712 was purified by anion-exchange and gel-filtration chromatography techniques.

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General info

Function

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (Probable). Parts of the peripheral arm of the enzyme, where the electrons from NADH are accepted by flavin mononucleotide (FMN) and then passed along a chain of iron-sulfur clusters by electron tunnelling to the final acceptor ubiquinone (Probable). Contains one iron-sulfur cluster (Probable).

Sequence similarities

Belongs to the complex I 24 kDa subunit family.

Subcellular localisation

Mitochondrion inner membrane

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Product protocols

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Target data

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (Probable). Parts of the peripheral arm of the enzyme, where the electrons from NADH are accepted by flavin mononucleotide (FMN) and then passed along a chain of iron-sulfur clusters by electron tunnelling to the final acceptor ubiquinone (Probable). Contains one iron-sulfur cluster (Probable).
See full target information NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial
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Complementary Products

Primary Antibodies

AB137757

Anti-CYC1 antibody

Western blot - Anti-CYC1 antibody (AB137757)

  • 0
  • 0
Primary Antibodies

AB183715

Anti-NDUFV2 antibody [EPR15351(B)]

RabMAb|Recombinant|KO Validated

Western blot - Anti-NDUFV2 antibody [EPR15351(B)] (AB183715)

  • 5
  • 1
Primary Antibodies

AB134045

Anti-CD63 antibody [EPR5702] - Late Endosome Marker

RabMAb|Recombinant|KO Validated|Lab Essentials|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [EPR5702] - Late Endosome Marker (AB134045)

  • 3
  • 6
Primary Antibodies

AB125011

Anti-TSG101 antibody [EPR7130(B)]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry - Anti-TSG101 antibody [EPR7130(B)] (AB125011)

  • 4
  • 10

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