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Recombinant Human NECAP2 protein is a Human Full Length protein, in the 1 to 263 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.

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Images

SDS-PAGE - Recombinant Human NECAP2 protein (AB104638), expandable thumbnail

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, MS

Biologically active

No

Amino acid sequence

M G S S H H H H H H S S G L V P R G S H M E E S G Y E S V L C V K P D V H V Y R I P P R A T N R G Y R A A E W Q L D Q P S W S G R L R I T A K G Q M A Y I K L E D R T S G E L F A Q A P V D Q F P G T A V E S V T D S S R Y F V I R I E D G N G R R A F I G I G F G D R G D A F D F N V A L Q D H F K W V K Q Q C E F A K Q A Q N P D Q G P K L D L G F K E G Q T I K L N I A N M K K K E G A A G N P R V R P A S T G G L S L L P P P P G G K T S T L I P P P G E Q L A V G G S L V Q P A V A P S S G G A P V P W P Q P N P A T A D I W G D F T K S T G S T S S Q T Q P G T G W V Q F

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

MS

Reactivity

Reacts

Dilution info

-

Notes

-

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Alternative names

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Recombinant Human NECAP2 protein is a Human Full Length protein, in the 1 to 263 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Applications

SDS-PAGE, MS

Accession
Q9NVZ3-1
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Sequence info

Amino acid sequence

M G S S H H H H H H S S G L V P R G S H M E E S G Y E S V L C V K P D V H V Y R I P P R A T N R G Y R A A E W Q L D Q P S W S G R L R I T A K G Q M A Y I K L E D R T S G E L F A Q A P V D Q F P G T A V E S V T D S S R Y F V I R I E D G N G R R A F I G I G F G D R G D A F D F N V A L Q D H F K W V K Q Q C E F A K Q A Q N P D Q G P K L D L G F K E G Q T I K L N I A N M K K K E G A A G N P R V R P A S T G G L S L L P P P P G G K T S T L I P P P G E Q L A V G G S L V Q P A V A P S S G G A P V P W P Q P N P A T A D I W G D F T K S T G S T S S Q T Q P G T G W V Q F

Accession

Q9NVZ3

Protein length

Full Length

Predicted molecular weight

30.5 kDa

Amino acids

1 to 263

Nature

Recombinant

Tags

His tag N-Terminus

Specifications

Form

Liquid

Additional notes

ab104638 is purified using conventional chromatography techniques.

General info

Function

Involved in endocytosis.

Sequence similarities

Belongs to the NECAP family.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

NECAP2 also known as NECAP endocytosis associated 2 is a protein that plays an important role in endocytic processes. Structurally it weighs approximately 27 kDa. This protein is highly expressed in the brain indicating its potential importance in neural activities. NECAP2 contains a phosphoinositide-binding PH domain and a C-terminal DPF motif which help it interact with other endocytic proteins.

Biological function summary

NECAP2 contributes to the efficiency of clathrin-mediated endocytosis. It acts as an accessory protein involved in the formation of clathrin-coated pits and vesicles. Part of the endocytic machinery NECAP2 works with AP-2 adaptor complex a significant player in endocytosis. Its interaction with clathrin and other adaptor proteins suggests that NECAP2's function is essential for regulating receptor-mediated internalization of molecules.

Pathways

NECAP2 participates in the clathrin-mediated endocytic pathway which is important for cellular nutrient uptake receptor recycling and signal transduction. NECAP2 closely interacts with proteins like clathrin and AP-2 helping modulate vesicle formation. In addition to its role in endocytosis NECAP2 might influence other cellular processes like signal transduction by affecting how specific receptors are recycled on cell membranes.

Associated diseases and disorders

NECAP2 has associations with neurological disorders due to its high expression in the brain and involvement in brain-specific endocytic pathways. Alterations in its normal function can relate to conditions like Alzheimer’s disease where endocytic processing of amyloid precursor protein is important. Moreover NECAP2's dysregulation could also influence schizophrenia through its interactions with proteins involved in synaptic vesicle endocytosis and neurotransmitter release.

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1 product image

  • SDS-PAGE - Recombinant Human NECAP2 protein (ab104638), expandable thumbnail

    SDS-PAGE - Recombinant Human NECAP2 protein (ab104638)

    15% SDS-PAGE analysis of 3μg ab104638.

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Product protocols

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