Recombinant human Nectin 3 protein (His tag) is a Human Fragment protein, in the 1 to 400 aa range, expressed in HEK 293, with >98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >98% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M A R T L R P S P L C P G G G K A Q L S S A S L L G A G L L L Q P P T P P P L L L L L F P L L L F S R L C G A L A G P I I V E P H V T A V W G K N V S L K C L I E V N E T I T Q I S W E K I H G K S S Q T V A V H H P Q Y G F S V Q G E Y Q G R V L F K N Y S L N D A T I T L H N I G F S D S G K Y I C K A V T F P L G N A Q S S T T V T V L V E P T V S L I K G P D S L I D G G N E T V A A I C I A A T G K P V A H I D W E G D L G E M E S T T T S F P N E T A T I I S Q Y K L F P T R F A R G R R I T C V V K H P A L E K D I R Y S F I L D I Q Y A P E V S V T G Y D G N W F V G R K G V N L K C N A D A N P P P F K S V W S R L D G Q W P D G L L A S D N T L H F V H P L T F N Y S G V Y I C K V T N S L G Q R S D Q K V I Y I S D P P T T T T L Q P T I Q W H P S T A D I E D L A T E P K K L P F P L S T L A T I K D D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cell adhesion molecule that promotes cell-cell adhesion through heterophilic trans-interactions with nectins-like or other nectins, such as trans-interaction with NECTIN2 at Sertoli-spermatid junctions (PubMed:16216929). Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1 (PubMed:16216929). Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation (PubMed:16216929). Involved in axon guidance by promoting contacts between the commissural axons and the floor plate cells (By similarity). Also involved in the formation of cell-cell junctions, including adherens junctions and synapses (By similarity). Promotes formation of checkerboard-like cellular pattern of hair cells and supporting cells in the auditory epithelium via heterophilic interaction with NECTIN1: NECTIN1 is present in the membrane of hair cells and associates with NECTIN3 on supporting cells, thereby mediating heterotypic adhesion between these two cell types (By similarity). Plays a role in the morphology of the ciliary body (By similarity).
Alternative names
CD113, PRR3, PVRL3, NECTIN3, Nectin-3, CDw113, Nectin cell adhesion molecule 3, Poliovirus receptor-related protein 3
Recommended products
Recombinant human Nectin 3 protein (His tag) is a Human Fragment protein, in the 1 to 400 aa range, expressed in HEK 293, with >98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >98% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Measured by its binding ability in a functional ELISA.
Immobilized recombinant human PVRL3 at 1 μg/ml (100 μl/well) can bind biotinylated Nectin-1 with a linear range of 6.4-800 ng/ml.
- Accession
- Q9NQS3-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- M A R T L R P S P L C P G G G K A Q L S S A S L L G A G L L L Q P P T P P P L L L L L F P L L L F S R L C G A L A G P I I V E P H V T A V W G K N V S L K C L I E V N E T I T Q I S W E K I H G K S S Q T V A V H H P Q Y G F S V Q G E Y Q G R V L F K N Y S L N D A T I T L H N I G F S D S G K Y I C K A V T F P L G N A Q S S T T V T V L V E P T V S L I K G P D S L I D G G N E T V A A I C I A A T G K P V A H I D W E G D L G E M E S T T T S F P N E T A T I I S Q Y K L F P T R F A R G R R I T C V V K H P A L E K D I R Y S F I L D I Q Y A P E V S V T G Y D G N W F V G R K G V N L K C N A D A N P P P F K S V W S R L D G Q W P D G L L A S D N T L H F V H P L T F N Y S G V Y I C K V T N S L G Q R S D Q K V I Y I S D P P T T T T L Q P T I Q W H P S T A D I E D L A T E P K K L P F P L S T L A T I K D D
- Accession
- Q9NQS3
- Protein length
- Fragment
- Predicted molecular weight
- 39.3 kDa
- Amino acids
- 1 to 400
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Cell adhesion molecule that promotes cell-cell adhesion through heterophilic trans-interactions with nectins-like or other nectins, such as trans-interaction with NECTIN2 at Sertoli-spermatid junctions (PubMed:16216929). Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1 (PubMed:16216929). Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation (PubMed:16216929). Involved in axon guidance by promoting contacts between the commissural axons and the floor plate cells (By similarity). Also involved in the formation of cell-cell junctions, including adherens junctions and synapses (By similarity). Promotes formation of checkerboard-like cellular pattern of hair cells and supporting cells in the auditory epithelium via heterophilic interaction with NECTIN1: NECTIN1 is present in the membrane of hair cells and associates with NECTIN3 on supporting cells, thereby mediating heterotypic adhesion between these two cell types (By similarity). Plays a role in the morphology of the ciliary body (By similarity).
- Sequence similarities
Belongs to the nectin family.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Nectin-3 also known as CD113 or PVRL3 is an immunoglobulin-like cell adhesion molecule. It plays an important role in cell-cell adhesion by forming homo- and heterophilic trans-dimers with other nectins and nectin-like molecules. Nectin-3 has a molecular mass of approximately 83 kDa. Its expression is generally observed in neural tissues such as the brain as well as in testis and various epithelial tissues. This distribution allows it to contribute to diverse cellular interactions critical for tissue organization and development.
- Biological function summary
Nectin-3 participates in the formation and maintenance of adherens junctions by interacting with cadherins and actin filaments. It is a member of the Nectin family which includes Nectin-1 Nectin-2 and Nectin-4 known for their roles in cell adhesion. The function of Nectin-3 is integral to the establishment of tight junctions contributing to the structural integrity and function of epithelial and endothelial cells. It often works as part of a complex with afadin which supports its connection to the actin cytoskeleton.
- Pathways
Nectin-3 acts in the formation of synapses and is involved in signaling pathways related to cell adhesion such as the actin cytoskeleton regulation pathway. It plays a significant role in the activation of the Ras and Rac small GTPase signaling pathways facilitating changes in cytoskeletal dynamics during cell movement and morphogenesis. Nectin-3 closely associates with other proteins in the Nectin family such as Nectin-1 and Nectin-2 in these pathways enhancing intercellular communication.
- Associated diseases and disorders
Mutations or dysregulation of Nectin-3 have been linked to conditions like mental retardation and developmental abnormalities. It is particularly relevant in the context of intellectual disability where alterations in cell adhesion and signaling can impair proper neuronal connectivity and brain function. Furthermore Nectin-3 interacts with PVRL proteins highlighting its possible involvement in cancer progression and metastasis through altered cell adhesion properties.
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4 product images
Functional Studies - Recombinant human Nectin 3 protein (His tag) (ab276326)
Measured by its binding ability in a functional ELISA .
1. Immobilized recombinant human PVRL3 at 1 μg/ml (100 μl/well) can bind biotinylated Nectin-1 with a linear range of 6.4-800 ng/ml .
2. Immobilized recombinant human PVRL3 at 1 μg/ml (100 μl/well) can bind Nectin-1/ Fc chimera with a linear range of 0.156-5 ng/ml.
SDS-PAGE - Recombinant human Nectin 3 protein (His tag) (ab276326)
SDS-PAGE analysis of ab276326.
Functional Studies - Recombinant human Nectin 3 protein (His tag) (ab276326)
Measured by its binding ability in a functional ELISA.
Immobilized recombinant human PVRL3 at 1 μg/ml (100 μl/well) can bind Nectin-1/ Fc chimera with a linear range of 15-30 ng/ml.
Functional Studies - Recombinant human Nectin 3 protein (His tag) (ab276326)
Measured by its binding ability in a functional ELISA.
Immobilized recombinant human PVRL3 at 1 μg/ml (100 μl/well) can bind biotinylated Nectin-1 with a linear range of 6.4-800 ng/ml.
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