Recombinant Human NEDD4-2 protein is a Human Full Length protein, in the 1 to 911 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M A T G L G E P V Y G L S E D E G E S R I L R V K V V S G I D L A K K D I F G A S D P Y V K L S L Y V A D E N R E L A L V Q T K T I K K T L N P K W N E E F Y F R V N P S N H R L L F E V F D E N R L T R D D F L G Q V D V P L S H L P T E D P T M E R P Y T F K D F L L R P R S H K S R V K G F L R L K M A Y M P K N G G Q D E E N S D Q R D D M E H G W E V V D S N D S A S Q H Q E E L P P P P L P P G W E E K V D N L G R T Y Y V N H N N R T T Q W H R P S L M D V S S E S D N N I R Q I N Q E A A H R R F R S R R H I S E D L E P E P S E G G D V P E P W E T I S E E V N I A G D S L G L A L P P P P A S P G S R T S P Q E L S E E L S R R L Q I T P D S N G E Q F S S L I Q R E P S S R L R S C S V T D A V A E Q G H L P P L A E D G A S G S A T N S N N H L I E P Q I R R P R S L S S P T V T L S A P L E G A K D S P V R R A V K D T L S N P Q S P Q P S P Y N S P K P Q H K V T Q S F L P P G W E M R I A P N G R P F F I D H N T K T T T W E D P R L K F P V H M R S K T S L N P N D L G P L P P G W E E R I H L D G R T F Y I D H N S K I T Q W E D P R L Q N P A I T G P A V P Y S R E F K Q K Y D Y F R K K L K K P A D I P N R F E M K L H R N N I F E E S Y R R I M S V K R P D V L K A R L W I E F E S E K G L D Y G G V A R E W F F L L S K E M F N P Y Y G L F E Y S A T D N Y T L Q I N P N S G L C N E D H L S Y F T F I G R V A G L A V F H G K L L D G F F I R P F Y K M M L G K Q I T L N D M E S V D S E Y Y N S L K W I L E N D P T G L D L M F C I D E E N F G Q T Y Q V D L K P N G S E I M V T N E N K R E Y I D L V I Q W R F V N R V Q K Q M N A F L E G F T E L L P I D L I K I F D E N E L E L L M C G L G D V D V N D W R Q H S I Y K N G Y C P N H P V I Q W F W K A V L L M D A E K R I R L L Q F V T G T S R V P M N G F A E L Y G S N G P Q L F T I E Q W G S P E K L P R A H T C F N R L D L P P Y E T F E D L R E K L L M A V E N A Q G F E G V D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
E3 ubiquitin-protein ligase that mediates the polyubiquitination of lysine and cysteine residues on target proteins and is thereby implicated in the regulation of various signaling pathways including autophagy, innate immunity or DNA repair (PubMed:20064473, PubMed:31959741, PubMed:33608556). Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation (PubMed:15496141). Downregulates autophagy and cell growth by ubiquitinating and reducing cellular ULK1 or ASCT2 levels (PubMed:28820317, PubMed:31959741). Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, SCN2A/Nav1.2, SCN3A/Nav1.3, SCN5A/Nav1.5, SCN9A/Nav1.7, SCN10A/Nav1.8, KCNA3/Kv1.3, KCNH2, EAAT1, KCNQ2/Kv7.2, KCNQ3/Kv7.3 or CLC5 (PubMed:26363003, PubMed:27445338). Promotes ubiquitination and degradation of SGK1 and TNK2. Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1 (PubMed:25631046). Plays a role in dendrite formation by melanocytes (PubMed:23999003). Involved in the regulation of TOR signaling (PubMed:27694961). Ubiquitinates and regulates protein levels of NTRK1 once this one is activated by NGF (PubMed:27445338). Plays a role in antiviral innate immunity by catalyzing 'Lys-29'-linked cysteine ubiquitination of TRAF3, resulting in enhanced 'Lys-48' and 'Lys-63'-linked ubiquitination of TRAF3 (PubMed:33608556). Ubiquitinates TTYH2 and TTYH3 and regulates protein levels of TTYH2 (PubMed:18577513).
Alternative names
KIAA0439, NEDL3, NEDD4L, E3 ubiquitin-protein ligase NEDD4-like, HECT-type E3 ubiquitin transferase NED4L, NEDD4.2, Nedd4-2
Recommended products
Recombinant Human NEDD4-2 protein is a Human Full Length protein, in the 1 to 911 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- Q96PU5-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M A T G L G E P V Y G L S E D E G E S R I L R V K V V S G I D L A K K D I F G A S D P Y V K L S L Y V A D E N R E L A L V Q T K T I K K T L N P K W N E E F Y F R V N P S N H R L L F E V F D E N R L T R D D F L G Q V D V P L S H L P T E D P T M E R P Y T F K D F L L R P R S H K S R V K G F L R L K M A Y M P K N G G Q D E E N S D Q R D D M E H G W E V V D S N D S A S Q H Q E E L P P P P L P P G W E E K V D N L G R T Y Y V N H N N R T T Q W H R P S L M D V S S E S D N N I R Q I N Q E A A H R R F R S R R H I S E D L E P E P S E G G D V P E P W E T I S E E V N I A G D S L G L A L P P P P A S P G S R T S P Q E L S E E L S R R L Q I T P D S N G E Q F S S L I Q R E P S S R L R S C S V T D A V A E Q G H L P P L A E D G A S G S A T N S N N H L I E P Q I R R P R S L S S P T V T L S A P L E G A K D S P V R R A V K D T L S N P Q S P Q P S P Y N S P K P Q H K V T Q S F L P P G W E M R I A P N G R P F F I D H N T K T T T W E D P R L K F P V H M R S K T S L N P N D L G P L P P G W E E R I H L D G R T F Y I D H N S K I T Q W E D P R L Q N P A I T G P A V P Y S R E F K Q K Y D Y F R K K L K K P A D I P N R F E M K L H R N N I F E E S Y R R I M S V K R P D V L K A R L W I E F E S E K G L D Y G G V A R E W F F L L S K E M F N P Y Y G L F E Y S A T D N Y T L Q I N P N S G L C N E D H L S Y F T F I G R V A G L A V F H G K L L D G F F I R P F Y K M M L G K Q I T L N D M E S V D S E Y Y N S L K W I L E N D P T G L D L M F C I D E E N F G Q T Y Q V D L K P N G S E I M V T N E N K R E Y I D L V I Q W R F V N R V Q K Q M N A F L E G F T E L L P I D L I K I F D E N E L E L L M C G L G D V D V N D W R Q H S I Y K N G Y C P N H P V I Q W F W K A V L L M D A E K R I R L L Q F V T G T S R V P M N G F A E L Y G S N G P Q L F T I E Q W G S P E K L P R A H T C F N R L D L P P Y E T F E D L R E K L L M A V E N A Q G F E G V D
- Accession
- Q96PU5
- Protein length
- Full Length
- Predicted molecular weight
- 125.99 kDa
- Amino acids
- 1 to 911
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
E3 ubiquitin-protein ligase that mediates the polyubiquitination of lysine and cysteine residues on target proteins and is thereby implicated in the regulation of various signaling pathways including autophagy, innate immunity or DNA repair (PubMed:20064473, PubMed:31959741, PubMed:33608556). Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation (PubMed:15496141). Downregulates autophagy and cell growth by ubiquitinating and reducing cellular ULK1 or ASCT2 levels (PubMed:28820317, PubMed:31959741). Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, SCN2A/Nav1.2, SCN3A/Nav1.3, SCN5A/Nav1.5, SCN9A/Nav1.7, SCN10A/Nav1.8, KCNA3/Kv1.3, KCNH2, EAAT1, KCNQ2/Kv7.2, KCNQ3/Kv7.3 or CLC5 (PubMed:26363003, PubMed:27445338). Promotes ubiquitination and degradation of SGK1 and TNK2. Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1 (PubMed:25631046). Plays a role in dendrite formation by melanocytes (PubMed:23999003). Involved in the regulation of TOR signaling (PubMed:27694961). Ubiquitinates and regulates protein levels of NTRK1 once this one is activated by NGF (PubMed:27445338). Plays a role in antiviral innate immunity by catalyzing 'Lys-29'-linked cysteine ubiquitination of TRAF3, resulting in enhanced 'Lys-48' and 'Lys-63'-linked ubiquitination of TRAF3 (PubMed:33608556). Ubiquitinates TTYH2 and TTYH3 and regulates protein levels of TTYH2 (PubMed:18577513).
- Post-translational modifications
Phosphorylated by SGK1 or PKA; which impairs interaction with SCNN. Interaction with YWHAH inhibits dephosphorylation.
- Subcellular localisation
- Endosome, Multivesicular body
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NEDD4-2 also known as NEDD4L is a HECT domain-containing ubiquitin E3 ligase with a mass of approximately 120 kDa. It is expressed in various tissues especially in the kidney lung and colon. NEDD4-2 is involved in tagging proteins with ubiquitin marking them for degradation via the proteasome pathway. This protein helps regulate protein turnover by attaching ubiquitin chains to specific substrate proteins.
- Biological function summary
NEDD4-2 regulates key cellular processes by controlling the abundance of membrane proteins like ion channels and receptors. It binds and ubiquitinates specific targets like ENaC (epithelial sodium channel) significantly influencing electrolyte and fluid balance. NEDD4-2 often functions as part of multiprotein complexes that modulate cellular homeostasis. Efficient function of these complexes ensures cells maintain balance in their environment.
- Pathways
NEDD4-2 integrates into the MAPK and Wnt signaling pathways influencing diverse cellular outcomes like proliferation and apoptosis. Acting within these pathways NEDD4-2 associates with proteins such as Ras and β-catenin. This interaction influences cellular responses to external stimuli playing roles in cellular growth regulation and fate decisions.
- Associated diseases and disorders
Abnormalities in NEDD4-2 function are linked to conditions like Liddle's syndrome and hypertension. Mutations or dysregulation in NEDD4-2 can alter ENaC activity leading to increased sodium retention and contribute to high blood pressure. Moreover it connects to disorders like epithelial cancers through pathways involving β-catenin indicating its potential role in tumorigenesis. Research continues to explore these interactions to identify therapeutic targets.
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1 product image
SDS-PAGE - Recombinant Human NEDD4-2 protein (ab132337)
12.5% SDS-PAGE analysis of ab132337 stained with Coomassie Blue.
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