Recombinant human NEK1 protein (Active) is a Human Fragment protein, in the 1 to 477 aa range, expressed in Baculovirus infected Sf9, with >85% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M E K Y V R L Q K I G E G S F G K A I L V K S T E D G R Q Y V I K E I N I S R M S S K E R E E S R R E V A V L A N M K H P N I V Q Y R E S F E E N G S L Y I V M D Y C E G G D L F K R I N A Q K G V L F Q E D Q I L D W F V Q I C L A L K H V H D R K I L H R D I K S Q N I F L T K D G T V Q L G D F G I A R V L N S T V E L A R T C I G T P Y Y L S P E I C E N K P Y N N K S D I W A L G C V L Y E L C T L K H A F E A G S M K N L V L K I I S G S F P P V S L H Y S Y D L R S L V S Q L F K R N P R D R P S V N S I L E K G F I A K R I E K F L S P Q L I A E E F C L K T F S K F G S Q P I P A K R P A S G Q N S I S V M P A Q K I T K P A A K Y G I P L A Y K K Y G D K K L H E K K P L Q K H K Q A H Q T P E K R V N T G E E R R K I S E E A A R K R R L E F I E K E K K Q K D Q I I S L M K A E Q M K R Q E K E R L E R I N R A R E Q G W R N V L S A G G S G E V K A P F L G S G G T I A P S S F S S R G Q Y E H Y H A I F D Q M Q Q Q R A E D N E A K W K R E I Y G R G L P E R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Phosphorylates serines and threonines, but also appears to possess tyrosine kinase activity (PubMed:20230784). Involved in DNA damage checkpoint control and for proper DNA damage repair (PubMed:20230784). In response to injury that includes DNA damage, NEK1 phosphorylates VDAC1 to limit mitochondrial cell death (PubMed:20230784). May be implicated in the control of meiosis (By similarity). Involved in cilium assembly (PubMed:21211617).
Alternative names
KIAA1901, NEK1, Serine/threonine-protein kinase Nek1, Never in mitosis A-related kinase 1, Renal carcinoma antigen NY-REN-55, NimA-related protein kinase 1
Recommended products
Recombinant human NEK1 protein (Active) is a Human Fragment protein, in the 1 to 477 aa range, expressed in Baculovirus infected Sf9, with >85% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of ab268812 was 11 nmol/min/mg in a kinase assay using MBP protein as substrate.
- Accession
- Q96PY6-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- M E K Y V R L Q K I G E G S F G K A I L V K S T E D G R Q Y V I K E I N I S R M S S K E R E E S R R E V A V L A N M K H P N I V Q Y R E S F E E N G S L Y I V M D Y C E G G D L F K R I N A Q K G V L F Q E D Q I L D W F V Q I C L A L K H V H D R K I L H R D I K S Q N I F L T K D G T V Q L G D F G I A R V L N S T V E L A R T C I G T P Y Y L S P E I C E N K P Y N N K S D I W A L G C V L Y E L C T L K H A F E A G S M K N L V L K I I S G S F P P V S L H Y S Y D L R S L V S Q L F K R N P R D R P S V N S I L E K G F I A K R I E K F L S P Q L I A E E F C L K T F S K F G S Q P I P A K R P A S G Q N S I S V M P A Q K I T K P A A K Y G I P L A Y K K Y G D K K L H E K K P L Q K H K Q A H Q T P E K R V N T G E E R R K I S E E A A R K R R L E F I E K E K K Q K D Q I I S L M K A E Q M K R Q E K E R L E R I N R A R E Q G W R N V L S A G G S G E V K A P F L G S G G T I A P S S F S S R G Q Y E H Y H A I F D Q M Q Q Q R A E D N E A K W K R E I Y G R G L P E R
- Accession
- Q96PY6
- Protein length
- Fragment
- Amino acids
- 1 to 477
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Phosphorylates serines and threonines, but also appears to possess tyrosine kinase activity (PubMed:20230784). Involved in DNA damage checkpoint control and for proper DNA damage repair (PubMed:20230784). In response to injury that includes DNA damage, NEK1 phosphorylates VDAC1 to limit mitochondrial cell death (PubMed:20230784). May be implicated in the control of meiosis (By similarity). Involved in cilium assembly (PubMed:21211617).
- Sequence similarities
Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
- Subcellular localisation
- Nucleus, Cytoskeleton, Microtubule organizing center, Centrosome
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NEK1 also known as NIMA-related kinase 1 plays an important role in cell cycle regulation and DNA damage response. This serine/threonine-protein kinase has a molecular mass of approximately 140 kDa. NEK1 shows expression across various tissues particularly in the brain and testis. It functions by phosphorylating other proteins modifying their activity and ensuring proper cell division and stress response.
- Biological function summary
NEK1 coordinates critical processes such as ciliogenesis and mitochondrial function. It participates as part of protein complexes that help maintain cellular integrity. NEK1 interacts with proteins to regulate centrosome integrity and stability ensuring proper cell polarization and division. Additionally NEK1 has a role in the DNA damage checkpoint a mechanism that maintains genomic stability.
- Pathways
NEK1 integrates into the DNA damage response pathway and cilia-related signaling pathways. It interacts with key proteins such as ATR and ATM to mediate cell cycle arrest after DNA damage. NEK1 also influences the Hedgehog signaling pathway being important for ciliary function. These interactions underline its importance in cell cycle control and signaling.
- Associated diseases and disorders
NEK1 associates closely with amyotrophic lateral sclerosis (ALS) and polycystic kidney disease (PKD). Mutations in NEK1 have been linked to familial ALS indicating its role in neurodegenerative processes. In PKD NEK1 interacts with the polycystin proteins which are essential for kidney function leading to cyst formation when disrupted. These connections illustrate the impact of NEK1 dysregulation on multiple disease states.
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2 product images
Functional Studies - Recombinant human NEK1 protein (Active) (ab268812)
The specific activity of ab268812 was 11 nmol/min/mg in a kinase assay using MBP protein as substrate.
SDS-PAGE - Recombinant human NEK1 protein (Active) (ab268812)
SDS-PAGE analysis of ab268812.
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