Recombinant human NEK11 protein is a Human Full Length protein, in the 1 to 645 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, WB, FuncS.
Images
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Protein kinase which plays an important role in the G2/M checkpoint response to DNA damage. Controls degradation of CDC25A by directly phosphorylating it on residues whose phosphorylation is required for BTRC-mediated polyubiquitination and degradation.
Alternative names
Serine/threonine-protein kinase Nek11, Never in mitosis A-related kinase 11, NimA-related protein kinase 11, NEK11
Recommended products
Recombinant human NEK11 protein is a Human Full Length protein, in the 1 to 645 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, WB, FuncS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of ab125544 was determined to be 25 nmol/min/mg as per activity assay protocol.
- Accession
- Q8NG66-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.004% EGTA, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- Accession
- Q8NG66
- Protein length
- Full Length
- Predicted molecular weight
- 110 kDa
- Amino acids
- 1 to 645
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
Assessed by densitometry. Affinity purified.
General info
- Function
Protein kinase which plays an important role in the G2/M checkpoint response to DNA damage. Controls degradation of CDC25A by directly phosphorylating it on residues whose phosphorylation is required for BTRC-mediated polyubiquitination and degradation.
- Sequence similarities
Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
- Post-translational modifications
Phosphorylated by NEK2. Phosphorylation at Ser-273 is important for its activation.
- Subcellular localisation
- Nucleus, Nucleolus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Native Pig Myelin Basic Protein ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NEK11 also known as NIMA-related kinase 11 acts as a serine/threonine-protein kinase with important roles in cell cycle regulation. The protein has an approximate mass of 76 kDa and shows expression in various tissues including high levels in testis and liver. NEK11 localizes mainly in the nucleus where it performs its functions in response to cellular stress particularly during DNA damage.
- Biological function summary
NEK11 influences cell cycle control and integrates into mechanisms that manage DNA repair. It phosphorylates proteins involved in checkpoint regulation helping the cell respond to DNA damage. NEK11 does not operate alone but is part of a complex of proteins that ensure genomic stability under stress conditions. This complex role highlights its contribution to maintaining cell integrity.
- Pathways
NEK11 integrates into DNA damage response pathways such as the ATM-ATR signaling pathway and the p53 pathway. These pathways are important for cell cycle arrest and repair. NEK11 interacts closely with proteins like CHK1 and CHK2 which are well-known for their roles in managing cellular responses to DNA damage and preserving genomic stability.
- Associated diseases and disorders
NEK11 connects to disorders involving genomic instability like cancer. Abnormal NEK11 expression or function disrupts cell cycle regulation contributing to tumorigenesis. It also shows ties to proteins like p53 a tumor suppressor that plays a central role in diagnosing and understanding cancers. NEK11’s involvement in these pathways makes it a relevant target for cancer research and therapy.
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4 product images
Functional Studies - Recombinant human NEK11 protein (ab125544)
The specific activity of NEK11 (ab125544) was determined to be 21 nmol/min/mg as per activity assay protocol
SDS-PAGE - Recombinant human NEK11 protein (ab125544)
SDS PAGE analysis of ab125544
SDS-PAGE - Recombinant human NEK11 protein (ab125544)
SDS-PAGE analysis of ab125544.
Functional Studies - Recombinant human NEK11 protein (ab125544)
The specific activity of ab125544 was determined to be 25nmol/min/mg.
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