Recombinant human NEK4 protein (Active) is a Human Full Length protein, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >70% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Protein kinase that seems to act exclusively upon threonine residues (By similarity). Required for normal entry into proliferative arrest after a limited number of cell divisions, also called replicative senescence. Required for normal cell cycle arrest in response to double-stranded DNA damage.
Alternative names
STK2, NEK4, Serine/threonine-protein kinase Nek4, Never in mitosis A-related kinase 4, Serine/threonine-protein kinase 2, Serine/threonine-protein kinase NRK2, NimA-related protein kinase 4
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Recombinant human NEK4 protein (Active) is a Human Full Length protein, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >70% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of ab268813 was 15 nmol/min/mg in a kinase assay using myelin basic protein as substrate.
- Accession
- P51957-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- Accession
- P51957
- Protein length
- Full Length
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Protein kinase that seems to act exclusively upon threonine residues (By similarity). Required for normal entry into proliferative arrest after a limited number of cell divisions, also called replicative senescence. Required for normal cell cycle arrest in response to double-stranded DNA damage.
- Sequence similarities
Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NEK4 also known as NimA-related protein kinase 4 functions as a serine/threonine kinase involved in cell cycle regulation and ciliogenesis. It has a molecular weight of about 84 kDa. NEK4 is expressed widely in various tissues with significant expression in lung skeletal muscle and kidney. Its expression is important for proper cellular processes like mitosis and maintenance of the ciliary structure.
- Biological function summary
NEK4 participates in centrosome function and cilia dynamics playing an important role in maintaining cellular integrity. It associates with other proteins in a complex manner to facilitate cell cycle progression. NEK4 shows importance in regulating the stability of microtubules which affects the proper formation and function of cilia.
- Pathways
NEK4 integrates into major cellular pathways involved in cell cycle checkpoints and ciliogenesis. It is a part of the cell cycle regulation pathway influencing events necessary for DNA replication and mitotic progression. NEK4 also interacts with proteins like NEK1 which further modulates its activity in regulating cellular responses to DNA damage and ensuring correct cell division.
- Associated diseases and disorders
NEK4 links to conditions such as cancer and primary ciliary dyskinesia. In various cancers NEK4 expression may influence tumor growth and metastasis through its role in cell cycle regulation. In primary ciliary dyskinesia faults in NEK4-related proteins like NEK1 can affect cilia structure and function leading to clinical symptoms.
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2 product images
Functional Studies - Recombinant human NEK4 protein (Active) (ab268813)
The specific activity of ab268813 was 15 nmol/min/mg in a kinase assay using myelin basic protein as substrate.
SDS-PAGE - Recombinant human NEK4 protein (Active) (ab268813)
SDS-PAGE analysis of ab268813.
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