Recombinant human NEK5 protein is a Human Fragment protein, in the 1 to 508 aa range, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, WB, FuncS.
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Key facts
- Purity
- >70% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Alternative names
Serine/threonine-protein kinase Nek5, Never in mitosis A-related kinase 5, NimA-related protein kinase 5, NEK5
Recommended products
Recombinant human NEK5 protein is a Human Fragment protein, in the 1 to 508 aa range, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, WB, FuncS.
Key facts
- Purity
- >70% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of ab133140 was determined to be 100 nmol/min/mg.
- Accession
- Q6P3R8-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.005% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- Accession
- Q6P3R8
- Protein length
- Fragment
- Predicted molecular weight
- 59.1 kDa
- Amino acids
- 1 to 508
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Sequence similarities
Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Native Pig Myelin Basic Protein ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NEK5 known as NIMA-related kinase 5 is a serine/threonine-protein kinase with a molecular mass of approximately 71 kDa. It plays a dynamic role in cell cycle regulation. NEK5 is part of the NEK family of kinases which are similar to Aspergillus nidulans NIMA. It is mainly expressed in testes bone marrow and certain types of muscle tissue. Its kinase activity is important for the mitotic process helping to control cell division.
- Biological function summary
NEK5 participates in important steps of cell cycle progression by influencing cell cycle checkpoints. It does not belong to any well-characterized complex but its functional role in the cell cycle suggests interaction with other proteins during mitosis. Studies have shown that NEK5 can impact microtubule stabilization which affects proper chromosome segregation during cell division. This regulation is key to maintaining cellular integrity during proliferation.
- Pathways
NEK5 acts within the cell cycle pathway particularly influencing the mitotic phase. It shares functional pathways with other NEK family members such as NEK2 and NEK7 which also have roles in cell cycle control. These kinases interact with microtubule-associated proteins ensuring correct spindle assembly and function. NEK5's activity in these pathways ensures genomic stability by controlling mitotic progression.
- Associated diseases and disorders
NEK5 has been linked to certain types of cancer due to its role in cell division. Aberrant expression or mutation can lead to unchecked cellular proliferation increasing cancer risk. It also has potential connections to neurodegenerative disorders given its expression in neural tissues although further research is needed in this area. NEK2 another kinase from the same family also shows similar cancer-related pathologies through dysregulation of mitotic processes.
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4 product images
Functional Studies - Recombinant human NEK5 protein (ab133140)
The specific activity of NEK5 (ab133140) was determined to be 93 nmol/min/mg as per activity assay protocol
SDS-PAGE - Recombinant human NEK5 protein (ab133140)
SDS PAGE analysis of ab133140
SDS-PAGE - Recombinant human NEK5 protein (ab133140)
SDS-PAGE analysis of ab133140.
Functional Studies - Recombinant human NEK5 protein (ab133140)
The specific activity of ab133140 was determined to be 100 nmol/min/mg.
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