JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB60698

Recombinant human NEK6 protein

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant human NEK6 protein is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.

View Alternative Names

Serine/threonine-protein kinase Nek6, Never in mitosis A-related kinase 6, Protein kinase SID6-1512, NimA-related protein kinase 6, NEK6

4 Images
Functional Studies - Recombinant human NEK6 protein (AB60698)
  • FuncS

Unknown

Functional Studies - Recombinant human NEK6 protein (AB60698)

Sample Kinase Activity Plot.

Functional Studies - Recombinant human NEK6 protein (AB60698)
  • FuncS

Unknown

Functional Studies - Recombinant human NEK6 protein (AB60698)

The specific activity of NEK6 (ab60698) was determined to be 121 nmol/min/mg as per activity assay protocol

SDS-PAGE - Recombinant human NEK6 protein (AB60698)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human NEK6 protein (AB60698)

ab60698 on SDS-PAGE, MW ~63kDa.

SDS-PAGE - Recombinant human NEK6 protein (AB60698)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human NEK6 protein (AB60698)

SDS PAGE analysis of ab60698

Key facts

Purity

>90% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Active

Accession

Q9HC98

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

style
left-column

Complementary Products

Proteins & Peptides

AB271606

Recombinant human p38 alpha/MAPK14 protein (Active) (His tag N-Terminus)

Functional Studies - Recombinant human p38 alpha/MAPK14 protein (Active) (His tag N-Terminus) (AB271606)

  • 0
  • 0
Primary Antibodies

AB128908

Anti-FOXO4/AFX antibody [EPR5442]

RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-FOXO4/AFX antibody [EPR5442] (AB128908)

  • 5
  • 1
Primary Antibodies

AB133494

Anti-NEK6 antibody [EPR5283]

RabMAb|Recombinant|KO Validated

Western blot - Anti-NEK6 antibody [EPR5283] (AB133494)

  • 0
  • 0
Proteins & Peptides

AB269138

Recombinant human Wwp2 protein (Active)

Functional Studies - Recombinant human Wwp2 protein (Active) (AB269138)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
style
left-column

Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q9HC98","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein NIMA-related kinase 6 (NEK6) acts as a serine/threonine kinase involved in cell cycle regulation. It is also known as Never In Mitosis Gene A-related kinase 6. NEK6 has a molecular mass of approximately 40 kDa. Cells mainly express NEK6 in proliferating tissues. Its activity is dependent on phosphorylation by other kinases which allows NEK6 to proceed with mitosis by controlling different cellular processes.
Biological function summary

NEK6 plays a critical role in mitotic cell division by promoting progression through the G2/M phase of the cell cycle. Although NEK6 does not form part of a classical protein complex it does interact with various substrates to ensure successful mitosis. It stabilizes microtubule organization ensuring proper chromosomal segregation and cell cycle progression by facilitating microtubule nucleation and stability.

Pathways

NEK6 contributes to cell cycle control and microtubule dynamics being important within the context of the DNA damage response and repair pathways. NEK6 is associated with the p53 signaling pathway where it functions alongside related proteins such as NEK7. These kinases coordinate cellular responses to stress and damage ultimately maintaining genomic stability and preventing aberrant cell division.

NEK6 has been linked to oncogenesis especially in various types of cancer. Overexpression or dysregulation of NEK6 can promote tumor growth with studies showing its involvement in conditions such as breast cancer. NEK6 activity parallels oncogenic signals often interacting with proteins like p53 whose inactivation contributes to tumorigenesis. Understanding NEK6's role in these pathways may offer novel therapeutic targets for cancer treatment.
style
left-column

Specifications

Form

Liquid

Additional notes

Purity: >90% as determined by densitometry. Affinity purified.

style
left-column

General info

Function

Protein kinase which plays an important role in mitotic cell cycle progression (PubMed : 11516946, PubMed : 14563848). Required for chromosome segregation at metaphase-anaphase transition, robust mitotic spindle formation and cytokinesis (PubMed : 19414596). Phosphorylates ATF4, CIR1, PTN, RAD26L, RBBP6, RPS7, RPS6KB1, TRIP4, STAT3 and histones H1 and H3 (PubMed : 12054534, PubMed : 20873783). Phosphorylates KIF11 to promote mitotic spindle formation (PubMed : 19001501). Involved in G2/M phase cell cycle arrest induced by DNA damage (PubMed : 18728393). Inhibition of activity results in apoptosis. May contribute to tumorigenesis by suppressing p53/TP53-induced cancer cell senescence (PubMed : 21099361). Phosphorylates EML4 at 'Ser-144', promoting its dissociation from microtubules during mitosis which is required for efficient chromosome congression (PubMed : 31409757).

Sequence similarities

Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.

Post-translational modifications

Autophosphorylated. Phosphorylation at Ser-206 is required for its activation. Phosphorylated upon IR or UV-induced DNA damage. Phosphorylated by CHEK1 and CHEK2. Interaction with APBB1 down-regulates phosphorylation at Thr-210.

style
left-column

Product protocols

style
left-column

Target data

Protein kinase which plays an important role in mitotic cell cycle progression (PubMed : 11516946, PubMed : 14563848). Required for chromosome segregation at metaphase-anaphase transition, robust mitotic spindle formation and cytokinesis (PubMed : 19414596). Phosphorylates ATF4, CIR1, PTN, RAD26L, RBBP6, RPS7, RPS6KB1, TRIP4, STAT3 and histones H1 and H3 (PubMed : 12054534, PubMed : 20873783). Phosphorylates KIF11 to promote mitotic spindle formation (PubMed : 19001501). Involved in G2/M phase cell cycle arrest induced by DNA damage (PubMed : 18728393). Inhibition of activity results in apoptosis. May contribute to tumorigenesis by suppressing p53/TP53-induced cancer cell senescence (PubMed : 21099361). Phosphorylates EML4 at 'Ser-144', promoting its dissociation from microtubules during mitosis which is required for efficient chromosome congression (PubMed : 31409757).
See full target information Serine/threonine-protein kinase Nek6
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com