Recombinant Human NEK7 protein is a Human Full Length protein, in the 1 to 302 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S H M D E Q S Q G M Q G P P V P Q F Q P Q K A L R P D M G Y N T L A N F R I E K K I G R G Q F S E V Y R A A C L L D G V P V A L K K V Q I F D L M D A K A R A D C I K E I D L L K Q L N H P N V I K Y Y A S F I E D N E L N I V L E L A D A G D L S R M I K H F K K Q K R L I P E R T V W K Y F V Q L C S A L E H M H S R R V M H R D I K P A N V F I T A T G V V K L G D L G L G R F F S S K T T A A H S L V G T P Y Y M S P E R I H E N G Y N F K S D I W S L G C L L Y E M A A L Q S P F Y G D K M N L Y S L C K K I E Q C D Y P P L P S D H Y S E E L R Q L V N M C I N P D P E K R P D V T Y V Y D V A K R M H A C T A S S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Protein kinase which plays an important role in mitotic cell cycle progression (PubMed:17101132, PubMed:19941817, PubMed:31409757). Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis (PubMed:17586473, PubMed:19414596, PubMed:19941817, PubMed:26522158, PubMed:31409757). Phosphorylates EML4 at 'Ser-146', promoting its dissociation from microtubules during mitosis which is required for efficient chromosome congression (PubMed:31409757). Phosphorylates RPS6KB1 (By similarity). Acts as an essential activator of the NLRP3 inflammasome assembly independently of its kinase activity (PubMed:26642356, PubMed:36442502). Acts by unlocking NLRP3 following NLRP3 tranlocation into the microtubule organizing center (MTOC), relieving NLRP3 autoinhibition and promoting formation of the NLRP3:PYCARD complex, and activation of CASP1 (PubMed:26642356, PubMed:31189953, PubMed:36442502). Serves as a cellular switch that enforces mutual exclusivity of the inflammasome response and cell division: interaction with NEK9 prevents interaction with NLRP3 and activation of the inflammasome during mitosis (PubMed:26642356, PubMed:31189953).
Alternative names
Serine/threonine-protein kinase Nek7, Never in mitosis A-related kinase 7, NimA-related protein kinase 7, NEK7
Recommended products
Recombinant Human NEK7 protein is a Human Full Length protein, in the 1 to 302 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q8TDX7-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S H M D E Q S Q G M Q G P P V P Q F Q P Q K A L R P D M G Y N T L A N F R I E K K I G R G Q F S E V Y R A A C L L D G V P V A L K K V Q I F D L M D A K A R A D C I K E I D L L K Q L N H P N V I K Y Y A S F I E D N E L N I V L E L A D A G D L S R M I K H F K K Q K R L I P E R T V W K Y F V Q L C S A L E H M H S R R V M H R D I K P A N V F I T A T G V V K L G D L G L G R F F S S K T T A A H S L V G T P Y Y M S P E R I H E N G Y N F K S D I W S L G C L L Y E M A A L Q S P F Y G D K M N L Y S L C K K I E Q C D Y P P L P S D H Y S E E L R Q L V N M C I N P D P E K R P D V T Y V Y D V A K R M H A C T A S S
- Accession
- Q8TDX7
- Protein length
- Full Length
- Predicted molecular weight
- 37 kDa
- Amino acids
- 1 to 302
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab136361 was purified using conventional chromatography techniques.
General info
- Function
Protein kinase which plays an important role in mitotic cell cycle progression (PubMed:17101132, PubMed:19941817, PubMed:31409757). Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis (PubMed:17586473, PubMed:19414596, PubMed:19941817, PubMed:26522158, PubMed:31409757). Phosphorylates EML4 at 'Ser-146', promoting its dissociation from microtubules during mitosis which is required for efficient chromosome congression (PubMed:31409757). Phosphorylates RPS6KB1 (By similarity). Acts as an essential activator of the NLRP3 inflammasome assembly independently of its kinase activity (PubMed:26642356, PubMed:36442502). Acts by unlocking NLRP3 following NLRP3 tranlocation into the microtubule organizing center (MTOC), relieving NLRP3 autoinhibition and promoting formation of the NLRP3:PYCARD complex, and activation of CASP1 (PubMed:26642356, PubMed:31189953, PubMed:36442502). Serves as a cellular switch that enforces mutual exclusivity of the inflammasome response and cell division: interaction with NEK9 prevents interaction with NLRP3 and activation of the inflammasome during mitosis (PubMed:26642356, PubMed:31189953).
- Sequence similarities
Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
- Post-translational modifications
Phosphorylation at Ser-195 required for its activation.
- Subcellular localisation
- Nucleus, Cytoskeleton, Spindle pole, Microtubule organizing center, Centrosome
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The NIMA-related kinase 7 (NEK7) is a member of the NEK family of serine/threonine kinases and plays an important role in cell division. With a molecular mass of approximately 34 kDa the NEK7 protein is involved in mitosis by regulating microtubule organization. It is primarily expressed in various tissues including human and marmoset thyroid tissues. It remains mainly inactive until mitotic entry where its activity becomes essential for proper spindle formation and completion of mitosis.
- Biological function summary
Cell cycle progression heavily depends on NEK7's function. NEK7 interacts with NEDD1 in the microtubule-organizing center contributing to centrosome maturation and separation. This interaction makes NEK7 vital in the recruitment of γ-tubulin ring complex which is necessary for nucleating microtubules. NEK7 operates independently from the other NEK family members but shares involvement in centrosome-related activities.
- Pathways
NEK7 is influential in the regulation of the mitotic spindle assembly pathway and the interleukin-1 (IL-1) signaling pathway. It is closely related to proteins like NEK6 and NEK9 which also play roles in mitotic progression and cytokinesis. NEK7's involvement in these pathways indicates its integrative function in ensuring normal cell division and response to inflammatory signals.
- Associated diseases and disorders
NEK7 has implications in cancer and inflammatory ailments. NEK7 overexpression or mutation is often linked to tumorigenesis particularly in lung and brain cancers. Additionally NEK7 contributes to the activation of the NLRP3 inflammasome connecting it to inflammatory conditions like rheumatoid arthritis. The interplay with NLRP3 highlights the significance of NEK7 in inflammation-related diseases.
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SDS-PAGE - Recombinant Human NEK7 protein (ab136361)
15% SDS-PAGE analysis of ab136361 (3 μg)
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