Recombinant human NEK7 protein is a Human Full Length protein, in the 1 to 302 aa range, expressed in Baculovirus infected Sf9, with >=72% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
>=72% SDS-PAGE
- Expression system
Baculovirus infected Sf9 cells
- Tags
GST tag N-Terminus
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
Amino acid sequence
M D E Q S Q G M Q G P P V P Q F Q P Q K A L R P D M G Y N T L A N F R I E K K I G R G Q F S E V Y R A A C L L D G V P V A L K K V Q I F D L M D A K A R A D C I K E I D L L K Q L N H P N V I K Y Y A S F I E D N E L N I V L E L A D A G D L S R M I K H F K K Q K R L I P E R T V W K Y F V Q L C S A L E H M H S R R V M H R D I K P A N V F I T A T G V V K L G D L G L G R F F S S K T T A A H S L V G T P Y Y M S P E R I H E N G Y N F K S D I W S L G C L L Y E M A A L Q S P F Y G D K M N L Y S L C K K I E Q C D Y P P L P S D H Y S E E L R Q L V N M C I N P D P E K R P D V T Y V Y D V A K R M H A C T A S S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Protein kinase which plays an important role in mitotic cell cycle progression (PubMed:17101132). Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis (PubMed:17586473, PubMed:19414596). Phosphorylates RPS6KB1 (By similarity). Phosphorylates EML4 at 'Ser-146', promoting its dissociation from microtubules during mitosis which is required for efficient chromosome congression (PubMed:31409757).
Alternative names
Serine/threonine-protein kinase Nek7, Never in mitosis A-related kinase 7, NimA-related protein kinase 7, NEK7
Recommended products
Recombinant human NEK7 protein is a Human Full Length protein, in the 1 to 302 aa range, expressed in Baculovirus infected Sf9, with >=72% purity and suitable for SDS-PAGE, FuncS.
Alternative names
Serine/threonine-protein kinase Nek7, Never in mitosis A-related kinase 7, NimA-related protein kinase 7, NEK7
Key facts
- Purity
>=72% SDS-PAGE
- Expression system
Baculovirus infected Sf9 cells
- Tags
GST tag N-Terminus
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
- Biological activity
Specific Activity: 47 pmol/min/μg
Assay was performed in a 25 μl reaction containing 40 mM Tris, pH 7.4, 20 mM MgCl2, 0.1 mg/ml BSA, 100 μM ATP, 0.2 mg/ml Casein and ab196067 for 50 min at 30°C.
- Accession
- Q8TDX7-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.49% Glutathione, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride
Sequence info
Amino acid sequence
M D E Q S Q G M Q G P P V P Q F Q P Q K A L R P D M G Y N T L A N F R I E K K I G R G Q F S E V Y R A A C L L D G V P V A L K K V Q I F D L M D A K A R A D C I K E I D L L K Q L N H P N V I K Y Y A S F I E D N E L N I V L E L A D A G D L S R M I K H F K K Q K R L I P E R T V W K Y F V Q L C S A L E H M H S R R V M H R D I K P A N V F I T A T G V V K L G D L G L G R F F S S K T T A A H S L V G T P Y Y M S P E R I H E N G Y N F K S D I W S L G C L L Y E M A A L Q S P F Y G D K M N L Y S L C K K I E Q C D Y P P L P S D H Y S E E L R Q L V N M C I N P D P E K R P D V T Y V Y D V A K R M H A C T A S S
- Accession
- Protein length
Full Length
- Predicted molecular weight
61.4 kDa
- Amino acids
1 to 302
- Nature
Recombinant
- Tags
GST tag N-Terminus
Specifications
- Form
Liquid
- Additional notes
Affinity purified.
General info
- Function
Protein kinase which plays an important role in mitotic cell cycle progression (PubMed:17101132). Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis (PubMed:17586473, PubMed:19414596). Phosphorylates RPS6KB1 (By similarity). Phosphorylates EML4 at 'Ser-146', promoting its dissociation from microtubules during mitosis which is required for efficient chromosome congression (PubMed:31409757).
- Sequence similarities
Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
- Post-translational modifications
Phosphorylation at Ser-195 required for its activation.
- Subcellular localisation
Nucleus, Cytoskeleton, Spindle pole, Microtubule organizing center, Centrosome
Storage
- Shipped at conditions
Dry Ice
- Appropriate long-term storage conditions
-80°C
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- Activity summary
The NIMA-related kinase 7 (NEK7) is a member of the NEK family of serine/threonine kinases and plays an important role in cell division. With a molecular mass of approximately 34 kDa the NEK7 protein is involved in mitosis by regulating microtubule organization. It is primarily expressed in various tissues including human and marmoset thyroid tissues. It remains mainly inactive until mitotic entry where its activity becomes essential for proper spindle formation and completion of mitosis.
- Biological function summary
Cell cycle progression heavily depends on NEK7's function. NEK7 interacts with NEDD1 in the microtubule-organizing center contributing to centrosome maturation and separation. This interaction makes NEK7 vital in the recruitment of γ-tubulin ring complex which is necessary for nucleating microtubules. NEK7 operates independently from the other NEK family members but shares involvement in centrosome-related activities.
- Pathways
NEK7 is influential in the regulation of the mitotic spindle assembly pathway and the interleukin-1 (IL-1) signaling pathway. It is closely related to proteins like NEK6 and NEK9 which also play roles in mitotic progression and cytokinesis. NEK7's involvement in these pathways indicates its integrative function in ensuring normal cell division and response to inflammatory signals.
- Associated diseases and disorders
NEK7 has implications in cancer and inflammatory ailments. NEK7 overexpression or mutation is often linked to tumorigenesis particularly in lung and brain cancers. Additionally NEK7 contributes to the activation of the NLRP3 inflammasome connecting it to inflammatory conditions like rheumatoid arthritis. The interplay with NLRP3 highlights the significance of NEK7 in inflammation-related diseases.
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2 product images
SDS-PAGE - Recombinant human NEK7 protein (ab196067)
4-20% SDS-PAGE analysis of 2.98 μg ab196067 followed by Coomassie staining.
Functional Studies - Recombinant human NEK7 protein (ab196067)
Kinase assay using ab196067 showing specific activity as 47 pmol/min/μg.
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