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Recombinant Human NELFe protein is a Human Full Length protein, in the 1 to 380 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
Alternative names=Negative elongation factor E, NELF-E, RNA-binding protein RD, NELFE, RDBP, RD
- Purity
>85% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, MS
- Biologically active
No
- Sequence
M G S S H H H H H H S S G L V P R G S H M L V I P P G L S E E E E A L Q K K F N K L K K K K K A L L A L K K Q S S S S T T S Q G G V K R S L S E Q P V M D T A T A T E Q A K Q L V K S G A I S A I K A E T K N S G F K R S R T L E G K L K D P E K G P V P T F Q P F Q R S I S A D D D L Q E S S R R P Q R K S L Y E S F V S S S D R L R E L G P D G E E A E G P G A G D G P P R S F D W G Y E E R S G A H S S A S P P R S R S R D R S H E R N R D R D R D R E R D R D R D R D R D R E R D R D R D R D R D R D R E R D R D R E R D R D R D R E G P F R R S D S F P E R R A P R K G N T L Y V Y G E D M T P T L L R G A F S P F G N I I D L S M D P P R N C A F V T Y E K M E S A D Q A V A E L N G T Q V E S V Q L K V N I A R K Q P M L D A A T G K S V W G S L A V Q N S P K G C H R D K R T Q I V Y S D D V Y K E N L V D G F
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
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Recombinant Human NELFe protein is a Human Full Length protein, in the 1 to 380 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
Alternative names=Negative elongation factor E, NELF-E, RNA-binding protein RD, NELFE, RDBP, RD
Key facts
- Purity
>85% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, MS
- Biologically active
No
- Predicted molecular weight
45.4 kDa
- Amino acids
1 to 380
- Sequence
M G S S H H H H H H S S G L V P R G S H M L V I P P G L S E E E E A L Q K K F N K L K K K K K A L L A L K K Q S S S S T T S Q G G V K R S L S E Q P V M D T A T A T E Q A K Q L V K S G A I S A I K A E T K N S G F K R S R T L E G K L K D P E K G P V P T F Q P F Q R S I S A D D D L Q E S S R R P Q R K S L Y E S F V S S S D R L R E L G P D G E E A E G P G A G D G P P R S F D W G Y E E R S G A H S S A S P P R S R S R D R S H E R N R D R D R D R E R D R D R D R D R D R E R D R D R D R D R D R D R E R D R D R E R D R D R D R E G P F R R S D S F P E R R A P R K G N T L Y V Y G E D M T P T L L R G A F S P F G N I I D L S M D P P R N C A F V T Y E K M E S A D Q A V A E L N G T Q V E S V Q L K V N I A R K Q P M L D A A T G K S V W G S L A V Q N S P K G C H R D K R T Q I V Y S D D V Y K E N L V D G F
- Accession
- P18615-1
- Protein length
Full Length
- Animal free
No
- Nature
Recombinant
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Specifications
- Form
Liquid
- Additional notes
ab104637 is purified using conventional chromatography techniques.
General info
- Function
Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II (PubMed:10199401). The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex (PubMed:11940650, PubMed:12612062). Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA (PubMed:18303858, PubMed:27282391).
- Sequence similarities
Belongs to the RRM NELF-E family.
- Post-translational modifications
Sumoylated.
- Subcellular localisation
Nucleus
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
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