Recombinant Human NELFe protein is a Human Full Length protein, in the 1 to 380 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M L V I P P G L S E E E E A L Q K K F N K L K K K K K A L L A L K K Q S S S S T T S Q G G V K R S L S E Q P V M D T A T A T E Q A K Q L V K S G A I S A I K A E T K N S G F K R S R T L E G K L K D P E K G P V P T F Q P F Q R S I S A D D D L Q E S S R R P Q R K S L Y E S F V S S S D R L R E L G P D G E E A E G P G A G D G P P R S F D W G Y E E R S G A H S S A S P P R S R S R D R S H E R N R D R D R D R E R D R D R D R D R D R E R D R D R D R D R D R D R E R D R D R E R D R D R D R E G P F R R S D S F P E R R A P R K G N T L Y V Y G E D M T P T L L R G A F S P F G N I I D L S M D P P R N C A F V T Y E K M E S A D Q A V A E L N G T Q V E S V Q L K V N I A R K Q P M L D A A T G K S V W G S L A V Q N S P K G C H R D K R T Q I V Y S D D V Y K E N L V D G F
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II (PubMed:10199401, PubMed:27256882). The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex (PubMed:11940650, PubMed:12612062, PubMed:27256882). Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA (PubMed:18303858, PubMed:27256882, PubMed:27282391). (Microbial infection) The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II.
Alternative names
RD, RDBP, NELFE, Negative elongation factor E, NELF-E, RNA-binding protein RD
Recommended products
Recombinant Human NELFe protein is a Human Full Length protein, in the 1 to 380 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P18615-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M L V I P P G L S E E E E A L Q K K F N K L K K K K K A L L A L K K Q S S S S T T S Q G G V K R S L S E Q P V M D T A T A T E Q A K Q L V K S G A I S A I K A E T K N S G F K R S R T L E G K L K D P E K G P V P T F Q P F Q R S I S A D D D L Q E S S R R P Q R K S L Y E S F V S S S D R L R E L G P D G E E A E G P G A G D G P P R S F D W G Y E E R S G A H S S A S P P R S R S R D R S H E R N R D R D R D R E R D R D R D R D R D R E R D R D R D R D R D R D R E R D R D R E R D R D R D R E G P F R R S D S F P E R R A P R K G N T L Y V Y G E D M T P T L L R G A F S P F G N I I D L S M D P P R N C A F V T Y E K M E S A D Q A V A E L N G T Q V E S V Q L K V N I A R K Q P M L D A A T G K S V W G S L A V Q N S P K G C H R D K R T Q I V Y S D D V Y K E N L V D G F
- Accession
- P18615
- Protein length
- Full Length
- Predicted molecular weight
- 45.4 kDa
- Amino acids
- 1 to 380
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab104637 is purified using conventional chromatography techniques.
General info
- Function
Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II (PubMed:10199401, PubMed:27256882). The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex (PubMed:11940650, PubMed:12612062, PubMed:27256882). Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA (PubMed:18303858, PubMed:27256882, PubMed:27282391).
- Sequence similarities
Belongs to the RRM NELF-E family.
- Post-translational modifications
Phosphorylated by the P-TEFb complex at sites next to its RNA recognition motif, promoting its release from chromatin.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NELFe also known as Negative Elongation Factor E or NELF-A plays an important role in regulating transcription elongation. NELFe has a mass of approximately 66 kDa. Expression of NELFe occurs broadly across various tissues which suggests its involvement in numerous cellular processes. This protein forms part of the NELF complex which interacts with RNA Polymerase II pausing transcription at specific genes. NELFe along with other components contributes to the binding to nascent RNA and halting transcription temporarily.
- Biological function summary
NELFe influences gene expression by controlling the pausing of RNA Polymerase II which impacts the timing and localization of transcription. NELFe operates within a larger complex known as the Negative Elongation Factor complex that consists of NELF-A (NELFe) NELF-B NELF-C/D and NELF-E. This complex enables precise regulation of transcriptional pausing and subsequent release when appropriate signals arrive. The coordinated action of this complex ensures proper gene expression profiles necessary for development and cell differentiation.
- Pathways
NELFe fits prominently in the transcription regulation pathway where it controls RNA Polymerase II pausing and release. NELFe interacts with the protein P-TEFb (Positive Transcription Elongation Factor b) which phosphorylates NELF complex components to release the pause allowing elongation. Another significant pathway involves NELFe in the regulation of heat shock genes where the heat shock factor 1 (HSF1) modulates the transcriptional pause release in response to stress showing NELFe's role in cellular stress responses.
- Associated diseases and disorders
NELFe has links to cancer and viral infections where its dysregulation can affect normal gene expression. In certain cancers unregulated pausing or failure to release transcription elongation can contribute to uncontrolled cell proliferation. Furthermore in the context of human immunodeficiency virus (HIV) infections NELFe and its related NELF complex play a role in viral latency. The related protein Tat interacts with NELFe highlighting its importance in HIV transcription regulation. Understanding NELFe's role in these diseases provides insight into potential therapeutic targets for intervention.
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1 product image
SDS-PAGE - Recombinant Human NELFe protein (ab104637)
15% SDS-PAGE analysis of 3μg ab104637.
Note: Real molecular weight on SDS-PAGE is shifted up compared to predicted molecular weight.
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