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AB104637

Recombinant Human NELFe protein

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Recombinant Human NELFe protein is a Human Full Length protein, in the 1 to 380 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

RD, RDBP, NELFE, Negative elongation factor E, NELF-E, RNA-binding protein RD

1 Images
SDS-PAGE - Recombinant Human NELFe protein (AB104637)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human NELFe protein (AB104637)

15% SDS-PAGE analysis of 3μg ab104637.
Note : Real molecular weight on SDS-PAGE is shifted up compared to predicted molecular weight.

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

P18615

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMLVIPPGLSEEEEALQKKFNKLKKKKKALLALKKQSSSSTTSQGGVKRSLSEQPVMDTATATEQAKQLVKSGAISAIKAETKNSGFKRSRTLEGKLKDPEKGPVPTFQPFQRSISADDDLQESSRRPQRKSLYESFVSSSDRLRELGPDGEEAEGPGAGDGPPRSFDWGYEERSGAHSSASPPRSRSRDRSHERNRDRDRDRERDRDRDRDRDRERDRDRDRDRDRDRERDRDRERDRDRDREGPFRRSDSFPERRAPRKGNTLYVYGEDMTPTLLRGAFSPFGNIIDLSMDPPRNCAFVTYEKMESADQAVAELNGTQVESVQLKVNIARKQPMLDAATGKSVWGSLAVQNSPKGCHRDKRTQIVYSDDVYKENLVDGF","proteinLength":"Full Length","predictedMolecularWeight":"45.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":380,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P18615","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NELFe also known as Negative Elongation Factor E or NELF-A plays an important role in regulating transcription elongation. NELFe has a mass of approximately 66 kDa. Expression of NELFe occurs broadly across various tissues which suggests its involvement in numerous cellular processes. This protein forms part of the NELF complex which interacts with RNA Polymerase II pausing transcription at specific genes. NELFe along with other components contributes to the binding to nascent RNA and halting transcription temporarily.
Biological function summary

NELFe influences gene expression by controlling the pausing of RNA Polymerase II which impacts the timing and localization of transcription. NELFe operates within a larger complex known as the Negative Elongation Factor complex that consists of NELF-A (NELFe) NELF-B NELF-C/D and NELF-E. This complex enables precise regulation of transcriptional pausing and subsequent release when appropriate signals arrive. The coordinated action of this complex ensures proper gene expression profiles necessary for development and cell differentiation.

Pathways

NELFe fits prominently in the transcription regulation pathway where it controls RNA Polymerase II pausing and release. NELFe interacts with the protein P-TEFb (Positive Transcription Elongation Factor b) which phosphorylates NELF complex components to release the pause allowing elongation. Another significant pathway involves NELFe in the regulation of heat shock genes where the heat shock factor 1 (HSF1) modulates the transcriptional pause release in response to stress showing NELFe's role in cellular stress responses.

NELFe has links to cancer and viral infections where its dysregulation can affect normal gene expression. In certain cancers unregulated pausing or failure to release transcription elongation can contribute to uncontrolled cell proliferation. Furthermore in the context of human immunodeficiency virus (HIV) infections NELFe and its related NELF complex play a role in viral latency. The related protein Tat interacts with NELFe highlighting its importance in HIV transcription regulation. Understanding NELFe's role in these diseases provides insight into potential therapeutic targets for intervention.
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Specifications

Form

Liquid

Additional notes

ab104637 is purified using conventional chromatography techniques.

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General info

Function

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II (PubMed : 10199401, PubMed : 27256882). The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex (PubMed : 11940650, PubMed : 12612062, PubMed : 27256882). Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA (PubMed : 18303858, PubMed : 27256882, PubMed : 27282391).. (Microbial infection) The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II.

Sequence similarities

Belongs to the RRM NELF-E family.

Post-translational modifications

Phosphorylated by the P-TEFb complex at sites next to its RNA recognition motif, promoting its release from chromatin.. Sumoylated.. Poly-ADP-ribosylated by PARP1, thereby preventing RNA-binding and relieving transcription pausing.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II (PubMed : 10199401, PubMed : 27256882). The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex (PubMed : 11940650, PubMed : 12612062, PubMed : 27256882). Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA (PubMed : 18303858, PubMed : 27256882, PubMed : 27282391).. (Microbial infection) The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II.
See full target information NELFE
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