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AB164012

Recombinant Human Neprilysin-2 protein (GST tag N-Terminus)

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Recombinant Human Neprilysin-2 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 779 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

MELL1, MMEL2, NEP2, MMEL1, Membrane metallo-endopeptidase-like 1, Membrane metallo-endopeptidase-like 2, NEP2(m), Neprilysin II, Neprilysin-2, NEPII, NL2

1 Images
SDS-PAGE - Recombinant Human Neprilysin-2 protein (GST tag N-Terminus) (AB164012)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Neprilysin-2 protein (GST tag N-Terminus) (AB164012)

ab164012 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB

applications

Biologically active

No

Accession

Q495T6

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB213689

Anti-Neprilysin-2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neprilysin-2 antibody (AB213689)

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGKSEGPVGMVESAGRAGQKRPGFLEGGLLLLLLLVTAALVALGVLYADRRGKQLPRLASRLCFLQEERTFVKRKPRGIPEAQEVSEVCTTPGCVIAAARILQNMDPTTEPCDDFYQFACGGWLRRHVIPETNSRYSIFDVLRDELEVILKAVLENSTAKDRPAVEKARTLYRSCMNQSVIEKRGSQPLLDILEVVGGWPVAMDRWNETVGLEWELERQLALMNSQFNRRVLIDLFIWNDDQNSSRHIIYIDQPTLGMPSREYYFNGGSNRKVREAYLQFMVSVATLLREDANLPRDSCLVQEDMVQVLELETQLAKATVPQEERHDVIALYHRMGLEELQSQFGLKGFNWTLFIQTVLSSVKIKLLPDEEVVVYGIPYLQNLENIIDTYSARTIQNYLVWRLVLDRIGSLSQRFKDTRVNYRKALFGTMVEEVRWRECVGYVNSNMENAVGSLYVREAFPGDSKSMVRELIDKVRTVFVETLDELGWIDEESKKKAQEKAMSIREQIGHPDYILEETNRRLDEEYSNLNFSEDLYFENSLQNLKVGAQRSLRKLREKVDPNLWIIGAAVVNAFYSPNRNQIVFPAGILQPPFFSKEQPQALNFGGIGMVIGHEITHGFDDNGRNFDKNGNMMDWWSNFSTQHFREQSECMIYQYGNYSWDLADEQNVNGFNTLGENIADNGGVRQAYKAYLKWMAEGGKDQQLPGLDLTHEQLFFINYAQVWCGSYRPEFAIQSIKTDVHSPLKYRVLGSLQNLAAFADTFHCARGTPMHPKERCRVW","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":779,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q495T6","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neprilysin-2 also known as NEP2 or MMEL1 is a metalloendopeptidase enzyme similar to neprilysin. It has an approximate mass of 85 kDa. The enzyme is expressed in various tissues with a notable presence in the brain. NEP2 plays an important role in peptide degradation acting on a range of bioactive peptides by cleaving peptide bonds.
Biological function summary

NEP2 functions to break down peptides that are involved in signal transduction and cell communication. It participates in the regulation of neuropeptide signaling. NEP2 does not work alone but forms complexes with other proteins to perform its tasks efficiently. This activity allows it to maintain balance in peptide hormone levels and influence multiple physiological processes.

Pathways

Neprilysin-2 serves as a critical component in the metabolism of neuropeptides especially within the renin-angiotensin system and opioid peptide pathways. In these pathways NEP2 processes peptides like enkephalins and angiotensins directly interacting with other enzymes such as neprilysin and insulin-degrading enzyme. These interactions help modulate cardiovascular and nociceptive systems.

Neprilysin-2 associates with neurodegenerative diseases and cardiovascular disorders. Alteration in NEP2 activity is linked to Alzheimer's disease through its involvement in the degradation of amyloid-beta peptides. Its relation to neprilysin is significant because these enzymes together regulate peptides implicated in these conditions. Abnormal NEP2 function can lead to disrupted peptide balance contributing to disease pathogenesis.
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Specifications

Form

Liquid

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General info

Function

Metalloprotease involved in sperm function, possibly by modulating the processes of fertilization and early embryonic development. Degrades a broad variety of small peptides with a preference for peptides shorter than 3 kDa containing neutral bulky aliphatic or aromatic amino acid residues. Shares the same substrate specificity with MME and cleaves peptides at the same amide bond (By similarity).

Sequence similarities

Belongs to the peptidase M13 family.

Post-translational modifications

N-glycosylated.

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Product protocols

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Target data

Metalloprotease involved in sperm function, possibly by modulating the processes of fertilization and early embryonic development. Degrades a broad variety of small peptides with a preference for peptides shorter than 3 kDa containing neutral bulky aliphatic or aromatic amino acid residues. Shares the same substrate specificity with MME and cleaves peptides at the same amide bond (By similarity).
See full target information MMEL1
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