JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB116967

Recombinant Human NEU2 protein

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human NEU2 protein is a Human Fragment protein, in the 180 to 268 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

Sialidase-2, Cytosolic sialidase, N-acetyl-alpha-neuraminidase 2, NEU2

1 Images
SDS-PAGE - Recombinant Human NEU2 protein (AB116967)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human NEU2 protein (AB116967)

12.5% SDS-PAGE showing ab116967 at approximately 35.42kDa stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, SDS-PAGE, ELISA

applications

Biologically active

No

Accession

Q9Y3R4

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Recombinant protein.</p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"AYRKLHPIQRPIPSAFCFLSHDHGRTWARGHFVAQDTLECQVAEVETGEQRVVTLNARSHLRARVQAQSTNDGLDFQESQLVKKLVEPP","proteinLength":"Fragment","predictedMolecularWeight":"35.42 kDa","actualMolecularWeight":null,"aminoAcidEnd":268,"aminoAcidStart":180,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q9Y3R4","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The NEU2 gene encodes for the enzyme neuraminidase 2 also known as sialidase 2. Neuraminidase 2 has a mass of approximately 42 kDa. It plays a critical role in cleaving sialic acids from glycoproteins and glycolipids. NEU2 expression is mainly found in skeletal muscle and has been noted in other tissues such as the heart and kidney. These enzyme activities are important in cell-cell communication and modulation of immune responses.
Biological function summary

Neuraminidase 2 participates in the catabolism of sialoglycoconjugates. It is not a part of a larger protein complex but acts independently to remove sialic acids which can alter the properties and functions of substrates like glycoproteins on cell surfaces. These activities impact a number of biological processes including cellular signaling pathways which can influence immune and inflammatory responses.

Pathways

Neuraminidase 2 is involved in the catabolic process of the sialyl group as part of the glycosphingolipid metabolism. It is closely linked to the lysosomal pathway impacting the degradation and recycling of biomolecules. NEU2’s enzymatic work closely relates to other neuraminidases such as NEU1 and neurominidase-3 which have overlapping functions but different tissue distributions and substrate specificities.

Deficiencies or dysfunctions in neuraminidase 2 have associations with conditions like sialidosis and galactosialidosis. These disorders arise from improper degradation of sialic acids and can impact multiple systems in the body including the central nervous system. The protein NEU1 is another neuraminidase involved in similar disorders but it primarily contributes to lysosomal storage; together they underline the importance of proper sialic acid metabolism in health.
style
left-column

Specifications

Form

Liquid

style
left-column

General info

Function

Exo-alpha-sialidase that catalyzes the hydrolytic cleavage of the terminal sialic acid (N-acetylneuraminic acid, Neu5Ac) of a glycan moiety in the catabolism of glycolipids, glycoproteins and oligosacharides (PubMed : 14613940, PubMed : 22228546). Recognizes sialyl linkage positions of the glycan moiety as well as the supramolecular organization of the sialoglycoconjugate. Displays preference for alpha-(2->3)-sialylated GD1a and GT1B gangliosides over alpha-(2->8)-sialylated GD1b, in both monomeric forms and micelles. Hydrolyzes monomeric GM1 ganglioside, but has no activity toward the miscellar form (PubMed : 14613940). Has lower sialidase activity for glycoproteins such as fetuin and TF/transferrin that carry a mixture of alpha-(2->3) and alpha-(2->6)-sialyl linkages. Cleaves milk oligosaccharide alpha-(2->3)-sialyllactose, but is inactive toward alpha-(2->6)-sialyllactose isomer. Has no activity toward colominic acid, a homomer of alpha-(2->8)-linked Neu5Ac residues (PubMed : 14613940).

Sequence similarities

Belongs to the glycosyl hydrolase 33 family.

style
left-column

Product protocols

style
left-column

Target data

Exo-alpha-sialidase that catalyzes the hydrolytic cleavage of the terminal sialic acid (N-acetylneuraminic acid, Neu5Ac) of a glycan moiety in the catabolism of glycolipids, glycoproteins and oligosacharides (PubMed : 14613940, PubMed : 22228546). Recognizes sialyl linkage positions of the glycan moiety as well as the supramolecular organization of the sialoglycoconjugate. Displays preference for alpha-(2->3)-sialylated GD1a and GT1B gangliosides over alpha-(2->8)-sialylated GD1b, in both monomeric forms and micelles. Hydrolyzes monomeric GM1 ganglioside, but has no activity toward the miscellar form (PubMed : 14613940). Has lower sialidase activity for glycoproteins such as fetuin and TF/transferrin that carry a mixture of alpha-(2->3) and alpha-(2->6)-sialyl linkages. Cleaves milk oligosaccharide alpha-(2->3)-sialyllactose, but is inactive toward alpha-(2->6)-sialyllactose isomer. Has no activity toward colominic acid, a homomer of alpha-(2->8)-linked Neu5Ac residues (PubMed : 14613940).
See full target information NEU2
style
left-column
style
left-column
style
right-column

Alternative Products

Proteins & Peptides

AB267971

Recombinant Chinese hamster NEU2 protein (Tagged)

  • 0
  • 0

Complementary Products

Proteins & Peptides

AB316501

Recombinant Human CD3 Epsilon Protein Standard (His tag)

Sandwich ELISA - Recombinant Human CD3 Epsilon Protein Standard (His tag) (AB316501)

  • 0
  • 0
Proteins & Peptides

AB290100

Recombinant Human TNF Receptor II Protein (Active)

Premium Bioactive

Mass Spectrometry - Recombinant Human TNF Receptor II Protein (Active) (AB290100)

  • 0
  • 0
Proteins & Peptides

AB72490

Recombinant human MERTK protein

Functional Studies - Recombinant human MERTK protein (AB72490)

  • 0
  • 0
Proteins & Peptides

AB316459

Recombinant Human FGL1 Protein Standard

Sandwich ELISA - Recombinant Human FGL1 Protein Standard (AB316459)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com