JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB211934

Recombinant Human NeuroD1 protein

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human NeuroD1 protein is a Human Fragment protein, in the 2 to 356 aa range with >90% purity and suitable for SDS-PAGE. The predicted molecular weight of ab211934 protein is 40 kDa.

- Save time and ensure accurate results - use our NeuroD1 protein as a control

View Alternative Names

BHLHA3, NEUROD, NEUROD1, Neurogenic differentiation factor 1, NeuroD, NeuroD1, Class A basic helix-loop-helix protein 3, bHLHa3

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His-T7 tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q13562

Animal free

No

Carrier free

No

Species

Human

Storage buffer

Constituents: 0.32% Tris HCl

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Ensure the validity of your result using our recombinant human NeuroD1 protein ab211934 as a positive control in SDS-PAGE.

ab211934 was expressed in E.coli as inclusion bodies. The final product was refolded using a unique "temperature shift inclusion body refolding" technology, chromatographically purified and sterile-filtered.


Check out our protein gel staining guide for SDS-PAGE here
style
left-column

Sequence info

[{"sequence":"MASMTGGQQMGRGHHHHHHGNLYFQGGEFTKSYSESGLMGEPQPQGPPSWTDECLSSQDEEHEADKKEDDLEAMNAEEDSLRNGGEEEDEDEDLEEEEEEEEEDDDQKPKRRGPKKKKMTKARLERFKLRRMKANARERNRMHGLNAALDNLRKVVPCYSKTQKLSKIETLRLAKNYIWALSEILRSGKSPDLVSFVQTLCKGLSQPTTNLVAGCLQLNPRTFLPEQNQDMPPHLPTASASFPVHPYSYQSPGLPSPPYGTMDSSHVFHVKPPPHAYSAALEPFFESPLTDCTSPSFDGPLSPPLSINGNFSFKHEPSAEFEKNYAFTMHYPAATLAGAQSHGSIFSGTAAPRCEIPIDNIMSFDSHSHHERVMSAQLNAIFHDLEESGGGGSPGRRRRRRRRRRR","proteinLength":"Fragment","predictedMolecularWeight":"40 kDa","actualMolecularWeight":null,"aminoAcidEnd":356,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q13562","tags":[{"tag":"His-T7","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NeuroD1 also referred to as Neurogenic Differentiation 1 is a basic helix-loop-helix transcription factor with a molecular mass of approximately 40 kDa. This protein is expressed mainly in the central nervous system as well as in the pancreas. NeuroD1 plays a role in activating transcription by binding to DNA sequences in specific target genes. This process involves dimerization with E proteins facilitating its function as a transcriptional activator.
Biological function summary

NeuroD1 influences neural development and differentiation. It is vital in converting undifferentiated neural precursors into mature neurons ensuring proper brain formation. NeuroD1 forms part of a transcriptional complex with other neural transcription factors which coordinates the expression of genes needed for neuron specification. Apart from neurogenesis it regulates insulin gene expression in pancreatic beta cells linking neural functions to endocrine regulation.

Pathways

NeuroD1 functions in the neurogenesis pathway and interacts closely with the Notch signaling pathway. It plays a role by influencing the expression of genes essential for neuronal lineage commitment. In the insulin signaling pathway NeuroD1 cooperates with other transcription factors like Pdx1 and MafA managing the expression of insulin and other important beta-cell genes. Interactions with proteins like E47 increase its regulatory effects on these pathways.

Defects in NeuroD1 function connect to conditions such as diabetes mellitus and medulloblastoma. In diabetes mellitus NeuroD1 mutations can disturb insulin gene regulation impacting glucose metabolism severely. The connection with Pdx1 further magnifies its involvement in pancreatic beta-cell function. In medulloblastoma aberrant expression or mutations in NeuroD1 affect neuronal differentiation leading to tumor development. NeuroD1 influences cellular pathways interacting with proteins such as Sox9 which contributes to oncogenic processes in neural tissues.
style
left-column

Specifications

Form

Liquid

Additional notes

ab211934 was expressed in E.coli as inclusion bodies. The final product was refolded using a unique "temperature shift inclusion body refolding" technology, chromatographically purified and sterile-filtered.

style
left-column

General info

Function

Acts as a transcriptional activator : mediates transcriptional activation by binding to E box-containing promoter consensus core sequences 5'-CANNTG-3'. Associates with the p300/CBP transcription coactivator complex to stimulate transcription of the secretin gene as well as the gene encoding the cyclin-dependent kinase inhibitor CDKN1A. Contributes to the regulation of several cell differentiation pathways, like those that promote the formation of early retinal ganglion cells, inner ear sensory neurons, granule cells forming either the cerebellum or the dentate gyrus cell layer of the hippocampus, endocrine islet cells of the pancreas and enteroendocrine cells of the small intestine. Together with PAX6 or SIX3, is required for the regulation of amacrine cell fate specification. Also required for dendrite morphogenesis and maintenance in the cerebellar cortex. Associates with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis (By similarity).

Post-translational modifications

Phosphorylated. In islet cells, phosphorylated on Ser-274 upon glucose stimulation; which may be required for nuclear localization. In activated neurons, phosphorylated on Ser-335; which promotes dendritic growth. Phosphorylated by MAPK1; phosphorylation regulates heterodimerization and DNA-binding activities. Phosphorylation on Ser-266 and Ser-274 increases transactivation on the insulin promoter in glucose-stimulated insulinoma cells (By similarity).

Subcellular localisation

Nucleus

style
left-column

Product protocols

style
left-column

Target data

Acts as a transcriptional activator : mediates transcriptional activation by binding to E box-containing promoter consensus core sequences 5'-CANNTG-3'. Associates with the p300/CBP transcription coactivator complex to stimulate transcription of the secretin gene as well as the gene encoding the cyclin-dependent kinase inhibitor CDKN1A. Contributes to the regulation of several cell differentiation pathways, like those that promote the formation of early retinal ganglion cells, inner ear sensory neurons, granule cells forming either the cerebellum or the dentate gyrus cell layer of the hippocampus, endocrine islet cells of the pancreas and enteroendocrine cells of the small intestine. Together with PAX6 or SIX3, is required for the regulation of amacrine cell fate specification. Also required for dendrite morphogenesis and maintenance in the cerebellar cortex. Associates with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis (By similarity).
See full target information NEUROD1
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB18723

Anti-Doublecortin antibody

BOND RX™ Validated

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Doublecortin antibody (AB18723)

  • 5
  • 60
Proteins & Peptides

AB280753

Recombinant Human PD1 protein

Mass Spectrometry - Recombinant Human PD1 protein (AB280753)

  • 0
  • 0
Proteins & Peptides

AB280943

Recombinant human PD-L1 protein (Active)

Premium Bioactive

Sandwich ELISA - Recombinant human PD-L1 protein (Active) (AB280943)

  • 0
  • 0
Primary Antibodies

AB213725

Anti-NeuroD1 antibody [EPR20766]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-NeuroD1 antibody [EPR20766] (AB213725)

  • 4
  • 2

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com