Recombinant Human Neuropilin 1 protein is a Human Full Length protein, in the 23 to 644 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
R N D K C G D T I K I E S P G Y L T S P G Y P H S Y H P S E K C E W L I Q A P D P Y Q R I M I N F N P H F D L E D R D C K Y D Y V E V F D G E N E N G H F R G K F C G K I A P P P V V S S G P F L F I K F V S D Y E T H G A G F S I R Y E I F K R G P E C S Q N Y T T P S G V I K S P G F P E K Y P N S L E C T Y I V F A L K M S E I I L E F E S F D L E P D S N P P G G M F C R Y D R L E I R D G F P D V G P H I G R Y C G Q K T P G R I R S S S G I L S M V F Y T D S A I A K E G F S A N Y S V L Q S S V S E D F K C M E A L G M E S G E I H S D Q I T A S S Q Y S T N W S A E R S R L N Y P E N G W T P G E D S Y R E W I Q V D L G L L R F V T A V G T Q G A I S K E T K K K Y Y V K T Y K I D V S S N G E D W I T I K E G N K P V L F Q G N T N P T D V V V A V F P K P L I T R F V R I K P A T W E T G I S M R F E V Y G C K I T D Y P C S G M L G M V S G L I S D S Q I T S S N Q G D R N W M P E N I R L V T S R S G W A L P P A P H S Y I N E W L Q I D L G E E K I V R G I I I Q G G K H R E N K V F M R K F K I G Y S N N G S D W K M I M D D S K R K A K S F E G N N N Y D T P E L R T F P A L S T R F I R I Y P E R A T H G G L G L R M E L L G C E V E A P T A G P T T P N G N L V D E C D D D Q A N C H S G T G D D F Q L T G G T T V L A T E K P T V I D S T I Q S G I K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Cell-surface receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. Mediates the chemorepulsant activity of semaphorins (PubMed:10688880, PubMed:9288753, PubMed:9529250). Recognizes a C-end rule (CendR) motif R/KXXR/K on its ligands which causes cellular internalization and vascular leakage (PubMed:19805273). It binds to semaphorin 3A, the PLGF-2 isoform of PGF, the VEGF165 isoform of VEGFA and VEGFB (PubMed:10688880, PubMed:19805273, PubMed:9288753, PubMed:9529250). Coexpression with KDR results in increased VEGF165 binding to KDR as well as increased chemotaxis. Regulates VEGF-induced angiogenesis. Binding to VEGFA initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Regulates mitochondrial iron transport via interaction with ABCB8/MITOSUR (PubMed:30623799). (Microbial infection) Acts as a host factor for human coronavirus SARS-CoV-2 infection. Recognizes and binds to CendR motif RRAR on SARS-CoV-2 spike protein S1 which enhances SARS-CoV-2 infection. Isoform 2. Binds VEGF-165 and may inhibit its binding to cells (PubMed:10748121, PubMed:26503042). May induce apoptosis by sequestering VEGF-165 (PubMed:10748121). May bind as well various members of the semaphorin family. Its expression has an averse effect on blood vessel number and integrity.
Alternative names
CD304, NRP, VEGF165R, NRP1, Neuropilin-1, Vascular endothelial cell growth factor 165 receptor
Recommended products
Recombinant Human Neuropilin 1 protein is a Human Full Length protein, in the 23 to 644 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- O14786-2
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- R N D K C G D T I K I E S P G Y L T S P G Y P H S Y H P S E K C E W L I Q A P D P Y Q R I M I N F N P H F D L E D R D C K Y D Y V E V F D G E N E N G H F R G K F C G K I A P P P V V S S G P F L F I K F V S D Y E T H G A G F S I R Y E I F K R G P E C S Q N Y T T P S G V I K S P G F P E K Y P N S L E C T Y I V F A L K M S E I I L E F E S F D L E P D S N P P G G M F C R Y D R L E I R D G F P D V G P H I G R Y C G Q K T P G R I R S S S G I L S M V F Y T D S A I A K E G F S A N Y S V L Q S S V S E D F K C M E A L G M E S G E I H S D Q I T A S S Q Y S T N W S A E R S R L N Y P E N G W T P G E D S Y R E W I Q V D L G L L R F V T A V G T Q G A I S K E T K K K Y Y V K T Y K I D V S S N G E D W I T I K E G N K P V L F Q G N T N P T D V V V A V F P K P L I T R F V R I K P A T W E T G I S M R F E V Y G C K I T D Y P C S G M L G M V S G L I S D S Q I T S S N Q G D R N W M P E N I R L V T S R S G W A L P P A P H S Y I N E W L Q I D L G E E K I V R G I I I Q G G K H R E N K V F M R K F K I G Y S N N G S D W K M I M D D S K R K A K S F E G N N N Y D T P E L R T F P A L S T R F I R I Y P E R A T H G G L G L R M E L L G C E V E A P T A G P T T P N G N L V D E C D D D Q A N C H S G T G D D F Q L T G G T T V L A T E K P T V I D S T I Q S G I K
- Accession
- O14786
- Protein length
- Full Length
- Predicted molecular weight
- 94.16 kDa
- Amino acids
- 23 to 644
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Cell-surface receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. Mediates the chemorepulsant activity of semaphorins (PubMed:10688880, PubMed:9288753, PubMed:9529250). Recognizes a C-end rule (CendR) motif R/KXXR/K on its ligands which causes cellular internalization and vascular leakage (PubMed:19805273). It binds to semaphorin 3A, the PLGF-2 isoform of PGF, the VEGF165 isoform of VEGFA and VEGFB (PubMed:10688880, PubMed:19805273, PubMed:9288753, PubMed:9529250). Coexpression with KDR results in increased VEGF165 binding to KDR as well as increased chemotaxis. Regulates VEGF-induced angiogenesis. Binding to VEGFA initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Regulates mitochondrial iron transport via interaction with ABCB8/MITOSUR (PubMed:30623799).
- Sequence similarities
Belongs to the neuropilin family.
- Subcellular localisation
- Mitochondrion membrane
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Neuropilin-1 also known as NRP1 is a transmembrane protein with a significant role in the nervous and vascular systems. It has a molecular mass of approximately 130 kDa. Neuropilin-1 expression occurs broadly in tissues including neurons endothelial cells and tumor cells. Its structure includes a large extracellular domain that binds various ligands mediating several cellular functions. Neuropilin-1 is sometimes targeted in experiments using methods like neuropilin-1 ELISA and PE immunofluorescence to study its distribution and function in different tissues.
- Biological function summary
Neuropilin-1 serves as a co-receptor for both the Vascular Endothelial Growth Factor (VEGF) and Semaphorin family proteins. It plays an important role in processes such as angiogenesis axonal guidance and the immune system. Neuropilin-1 does not function alone; it forms complexes with neuropilin-2 and other receptors like Plexin and VEGFR enhancing signal transduction pathways for angiogenesis and neuronal development. This involvement allows cells to respond appropriately to their environment especially during organismal development and repair processes.
- Pathways
Neuropilin-1 facilitates interactions within the VEGF and Semaphorin pathways. In the VEGF pathway Neuropilin-1 enhances binding and signaling efficiency with VEGF closely working alongside VEGFR to promote endothelial cell survival migration and new blood vessel formation. In the Semaphorin pathway Neuropilin-1 interacts with Plexins mediating neuronal pathfinding and axonal growth. These interactions highlight Neuropilin-1's adaptive capabilities in various physiological processes critical for system development.
- Associated diseases and disorders
Neuropilin-1 is linked to pathological conditions like cancer and cardiovascular diseases. Neuropilin-1 overexpression is frequently observed in tumors driving cancer progression through enhanced angiogenesis and tissue invasion closely interacting with proteins like VEGF-A. In cardiovascular disease Neuropilin-1 contributes to abnormal blood vessel formation and stability. By studying Neuropilin-1 and other biomarkers like CD304 FITC researchers aim to develop therapeutic strategies targeting its role in these diseases.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human Neuropilin 1 protein (ab132179)
12.5% SDS-PAGE analysis of ab132179 stained with Coomassie Blue.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com