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Recombinant human Neuropilin 1 protein (Fc Chimera Active) is a Human Full Length protein, in the 22 to 644 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.

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Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

SDS-PAGE, FuncS

Biologically active

Yes

Sequence

F R N D K C G D T I K I E S P G Y L T S P G Y P H S Y H P

S E K C E W L I Q A P D P Y Q R I M I N F N P H F D L E D R D C K Y D Y V E V F D G E N E N G H F R

G K F C G K I A P P P V V S S G P F L F I K F V S D Y E T H G A G F S I R Y E I F K R G P E C S Q N

Y T T P S G V I K S P G F P E K Y P N S L E C T Y I V F V P K M S E I I L E F E S F D L E P D S N P

P G G M F C R Y D R L E I W D G F P D V G P H I G R Y C G Q K T P G R I R S S S G I L S M V F Y T D

S A I A K E G F S A N Y S V L Q S S V S E D F K C M E A L G M E S G E I H S D Q I T A S S Q Y S T N

W S A E R S R L N Y P E N G W T P G E D S Y R E W I Q V D L G L L R F V T A V G T Q G A I S K E T K

K K Y Y V K T Y K I D V S S N G E D W I T I K E G N K P V L F Q G N T N P T D V V V A V F P K P L I

T R F V R I K P A T W E T G I S M R F E V Y G C K I T D Y P C S G M L G M V S G L I S D S Q I T S S

N Q G D R N W M P E N I R L V T S R S G W A L P P A P H S Y I N E W L Q I D L G E E K I V R G I I I

Q G G K H R E N K V F M R K F K I G Y S N N G S D W K M I M D D S K R K A K S F E G N N N Y D T P E

L R T F P A L S T R F I R I Y P E R A T H G G L G L R M E L L G C E V E A P T A G P T T P N G N L V

D E C D D D Q A N C H S G T G D D F Q L T G G T T V L A T E K P T V I D S T I Q S G I K

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Shipping To:
United States

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
FuncS
Reactivity
Reacts
Dilution info
-
Notes

-

Images

Target data

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Recombinant human Neuropilin 1 protein (Fc Chimera Active) is a Human Full Length protein, in the 22 to 644 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Applications

SDS-PAGE, FuncS

Biological activity

Measured by its binding ability in a functional ELISA. Immobilized ab221023 at 2 μg/mL (100 μL/well) can bind Biotinylated Human VEGF165 with a linear range of 5-40 ng/mL.

Predicted molecular weight

96.5 kDa

Amino acids

22 to 644

Sequence

F R N D K C G D T I K I E S P G Y L T S P G Y P H S Y H P

S E K C E W L I Q A P D P Y Q R I M I N F N P H F D L E D R D C K Y D Y V E V F D G E N E N G H F R

G K F C G K I A P P P V V S S G P F L F I K F V S D Y E T H G A G F S I R Y E I F K R G P E C S Q N

Y T T P S G V I K S P G F P E K Y P N S L E C T Y I V F V P K M S E I I L E F E S F D L E P D S N P

P G G M F C R Y D R L E I W D G F P D V G P H I G R Y C G Q K T P G R I R S S S G I L S M V F Y T D

S A I A K E G F S A N Y S V L Q S S V S E D F K C M E A L G M E S G E I H S D Q I T A S S Q Y S T N

W S A E R S R L N Y P E N G W T P G E D S Y R E W I Q V D L G L L R F V T A V G T Q G A I S K E T K

K K Y Y V K T Y K I D V S S N G E D W I T I K E G N K P V L F Q G N T N P T D V V V A V F P K P L I

T R F V R I K P A T W E T G I S M R F E V Y G C K I T D Y P C S G M L G M V S G L I S D S Q I T S S

N Q G D R N W M P E N I R L V T S R S G W A L P P A P H S Y I N E W L Q I D L G E E K I V R G I I I

Q G G K H R E N K V F M R K F K I G Y S N N G S D W K M I M D D S K R K A K S F E G N N N Y D T P E

L R T F P A L S T R F I R I Y P E R A T H G G L G L R M E L L G C E V E A P T A G P T T P N G N L V

D E C D D D Q A N C H S G T G D D F Q L T G G T T V L A T E K P T V I D S T I Q S G I K

Accession
O14786-1
Protein length

Full Length

Animal free

No

Nature

Recombinant

Species

Human

Reconstitution

Reconstitute with sterile deionized water to a concentration of 200 µg/ml.

Concentration
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Storage buffer

pH: 7.4
Constituents: L-Arginine, Sodium chloride, 5% Trehalose, 0.75% Glycine, 0.61% Tris

Specifications

Form

Lyophilized

General info

Function

Cell-surface receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. Mediates the chemorepulsant activity of semaphorins (PubMed:9288753, PubMed:9529250, PubMed:10688880). Recognizes a C-end rule (CendR) motif R/KXXR/K on its ligands which causes cellular internalization and vascular leakage (PubMed:19805273). It binds to semaphorin 3A, the PLGF-2 isoform of PGF, the VEGF165 isoform of VEGFA and VEGFB (PubMed:9288753, PubMed:9529250, PubMed:10688880, PubMed:19805273). Coexpression with KDR results in increased VEGF165 binding to KDR as well as increased chemotaxis. Regulates VEGF-induced angiogenesis. Binding to VEGFA initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Regulates mitochondrial iron transport via interaction with ABCB8/MITOSUR (PubMed:30623799).

Sequence similarities

Belongs to the neuropilin family.

Subcellular localisation

Mitochondrion membrane

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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