Recombinant Human Neuroserpin protein (His tag) is a Human Full Length protein, in the 17 to 410 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
T G A T F P E E A I A D L S V N M Y N R L R A T G E D E N I L F S P L S I A L A M G M M E L G A Q G S T Q K E I R H S M G Y D S L K N G E E F S F L K E F S N M V T A K E S Q Y V M K I A N S L F V Q N G F H V N E E F L Q M M K K Y F N A A V N H V D F S Q N V A V A N Y I N K W V E N N T N N L V K D L V S P R D F D A A T Y L A L I N A V Y F K G N W K S Q F R P E N T R T F S F T K D D E S E V Q I P M M Y Q Q G E F Y Y G E F S D G S N E A G G I Y Q V L E I P Y E G D E I S M M L V L S R Q E V P L A T L E P L V K A Q L V E E W A N S V K K Q K V E V Y L P R F T V E Q E I D L K D V L K A L G I T E I F I K D A N L T G L S D N K E I F L S K A I H K S F L E V N E E G S E A A A V S G M I A I S R M A V L Y P Q V I V D H P F F F L I R N R R T G T I L F M G R V M H P E T M N T S G H D F E E L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Serine protease inhibitor that inhibits plasminogen activators and plasmin but not thrombin (PubMed:11880376, PubMed:19265707, PubMed:19285087, PubMed:26329378, PubMed:9442076). May be involved in the formation or reorganization of synaptic connections as well as for synaptic plasticity in the adult nervous system. May protect neurons from cell damage by tissue-type plasminogen activator (Probable).
Alternative names
PI12, SERPINI1, Neuroserpin, Peptidase inhibitor 12, Serpin I1, PI-12
Recommended products
Recombinant Human Neuroserpin protein (His tag) is a Human Full Length protein, in the 17 to 410 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q99574-1
- Animal free
- No
- Carrier free
- Yes
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- T G A T F P E E A I A D L S V N M Y N R L R A T G E D E N I L F S P L S I A L A M G M M E L G A Q G S T Q K E I R H S M G Y D S L K N G E E F S F L K E F S N M V T A K E S Q Y V M K I A N S L F V Q N G F H V N E E F L Q M M K K Y F N A A V N H V D F S Q N V A V A N Y I N K W V E N N T N N L V K D L V S P R D F D A A T Y L A L I N A V Y F K G N W K S Q F R P E N T R T F S F T K D D E S E V Q I P M M Y Q Q G E F Y Y G E F S D G S N E A G G I Y Q V L E I P Y E G D E I S M M L V L S R Q E V P L A T L E P L V K A Q L V E E W A N S V K K Q K V E V Y L P R F T V E Q E I D L K D V L K A L G I T E I F I K D A N L T G L S D N K E I F L S K A I H K S F L E V N E E G S E A A A V S G M I A I S R M A V L Y P Q V I V D H P F F F L I R N R R T G T I L F M G R V M H P E T M N T S G H D F E E L
- Accession
- Q99574
- Protein length
- Full Length
- Predicted molecular weight
- 46 kDa
- Amino acids
- 17 to 410
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Serine protease inhibitor that inhibits plasminogen activators and plasmin but not thrombin (PubMed:11880376, PubMed:19265707, PubMed:19285087, PubMed:26329378, PubMed:9442076). May be involved in the formation or reorganization of synaptic connections as well as for synaptic plasticity in the adult nervous system. May protect neurons from cell damage by tissue-type plasminogen activator (Probable).
- Sequence similarities
Belongs to the serpin family.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Neuroserpin also known by its alternate name Serpin I1 is a member of the serine protease inhibitor family. It weighs about 44 kDa. Neuroserpin expresses mainly in the nervous system particularly in the brain and spinal cord where it regulates proteolytic activity. It localizes to the endoplasmic reticulum and is secreted to the extracellular space influencing neural processes.
- Biological function summary
Neuroserpin plays an important role in nervous system development and maintenance. It inhibits tissue plasminogen activator (tPA) a protease involved in breaking down blood clots and controls extracellular matrix modifications. Neuroserpin does not form part of a complex but interacts with other key proteins for its function. Its regulation of tPA influences synaptic plasticity and protection of neurons from excessive proteolysis which is essential in maintaining proper brain function.
- Pathways
Neuroserpin's activity is significant in the plasminogen activation system and neurotrophic pathways. It influences the balance of proteolytic activities that affect cell migration and synaptic remodeling. Related proteins in these pathways include proteases like tPA and neurotrophins like brain-derived neurotrophic factor (BDNF) which support neuronal survival.
- Associated diseases and disorders
Mutations in neuroserpin are linked to familial encephalopathy with neuroserpin inclusion bodies (FENIB) and Alzheimer's disease. FENIB is caused by neuroserpin polymerization and accumulation leading to neurodegeneration. In Alzheimer's disease neuroserpin interaction with amyloid precursor protein pathways contributes to plaque formation and neuronal dysfunction. Neuroserpin's connection with defective tPA regulation underlies its association with these neurodegenerative conditions.
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SDS-PAGE - Recombinant Human Neuroserpin protein (His tag) (ab276361)
SDS-PAGE analysis of ab276361
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