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AB192292

Recombinant Human Neutrophil Bactericidal Protein BP30

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Recombinant Human Neutrophil Bactericidal Protein BP30 is a Human Full Length protein, in the 32 to 487 aa range, expressed in HEK 293 cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, HPLC.

View Alternative Names

Bactericidal permeability-increasing protein, BPI, CAP 57

Key facts

Purity

>95% SDS-PAGE

Purity is greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

HPLC, SDS-PAGE

applications

Biologically active

No

Accession

P17213

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4

storage-buffer

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Complementary Products

Primary Antibodies

AB249881

Anti-BPI antibody [EPR12350(B)] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-BPI antibody [EPR12350(B)] - BSA and Azide free (AB249881)

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Proteins & Peptides

AB163053

Recombinant Human Fbxw7 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human Fbxw7 protein (GST tag N-Terminus) (AB163053)

  • 0
  • 0
Primary Antibodies

AB154068

Anti-FBXO28 antibody

Immunocytochemistry/ Immunofluorescence - Anti-FBXO28 antibody (AB154068)

  • 0
  • 0
Primary Antibodies

AB1766

Anti-p55/DCAF1 antibody

  • 4
  • 2
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"linker":null,"sequence":"VNPGVVVRISQKGLDYASQQGTAALQKELKRIKIPDYSDSFKIKHLGKGHYSFYSMDIREFQLPSSQISMVPNVGLKFSISNANIKISGKWKAQKRFLKMSGNFDLSIEGMSISADLKLGSNPTSGKPTITCSSCSSHINSVHVHISKSKVGWLIQLFHKKIESALRNKMNSQVCEKVTNSVSSKLQPYFQTLPVMTKIDSVAGINYGLVAPPATTAETLDVQMKGEFYSENHHNPPPFAPPVMEFPAAHDRMVYLGLSDYFFNTAGLVYQEAGVLKMTLRDDMIPKESKFRLTTKFFGTFLPEVAKKFPNMKIQIHVSASTPPHLSVQPTGLTFYPAVDVQAFAVLPNSSLASLFLIGMHTTGSMEVSAESNRLVGELKLDRLLLELKHSNIGPFPVELLQDIMNYIVPILVLPRVNEKLQKGFPLPTPARVQLYNVVLQPHQNFLLFGADVVYKVDHHHHHH","proteinLength":"Full Length","predictedMolecularWeight":"51.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":487,"aminoAcidStart":32,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P17213","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Form
Lyophilized
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Reconstitute for long term storage
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neutrophil Bactericidal Protein BP30 also known as NGAL or LCN2 is a small protein with a mass of approximately 25 kDa. It is expressed chiefly in neutrophils but is also found in several other tissues including the liver lung and kidney. Mechanically BP30 plays a role in binding and transporting iron by sequestering bacterial siderophores which deprives bacteria of the iron essential for their growth. This activity is important for its function in the immune response.
Biological function summary

BP30 exerts antimicrobial effects by interfering with bacterial iron acquisition limiting bacterial growth and proliferation. It is not part of a complex; rather it functions as a monomer although it can form complexes with siderophores. Beyond its antimicrobial role BP30 also participates in cellular processes like apoptosis and inflammation. Its presence in various tissues points to its function as more systemically involved in defense mechanisms.

Pathways

BP30 interacts with several important biological pathways including the innate immune response and iron metabolism. Within the innate immune pathway it works alongside other antimicrobial peptides and proteins to enhance the body's first line of defense against pathogens. Additionally in the iron metabolism pathway BP30 modulates iron availability interacting with proteins such as transferrin and ferritin to balance iron homeostasis.

BP30 has a notable connection with kidney disease and some bacterial infections. In kidney disease BP30 serves as a biomarker for acute kidney injury indicating elevated levels when the kidneys are damaged. Its link to bacterial infections results from its primary function of iron sequestration depriving bacteria of necessary nutrients. BP30 is connected to hepcidin through its role in iron regulation which can influence the progression of these conditions.
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General info

Function

The cytotoxic action of BPI is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa.

Sequence similarities

Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family.

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Product protocols

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Target data

The cytotoxic action of BPI is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa.
See full target information BPI
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