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AB316527

Recombinant Human NFAT2 Protein Standard (His tag)

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Recombinant Human NFAT2 Protein Standard (His tag) is a Human Fragment protein, expressed in Escherichia coli, with >80%, suitable for SDS-PAGE, sELISA.

View Alternative Names

NFAT2, NFATC, NFATC1, NF-ATc1, NFATc1, NFAT transcription complex cytosolic component, NF-ATc, NFATc

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Sandwich ELISA - Recombinant Human NFAT2 Protein Standard (His tag) (AB316527)
  • sELISA

Supplier Data

Sandwich ELISA - Recombinant Human NFAT2 Protein Standard (His tag) (AB316527)

Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

SDS-PAGE - Recombinant Human NFAT2 Protein Standard (His tag) (AB316527)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human NFAT2 Protein Standard (His tag) (AB316527)

SDS-PAGE analysis of ab316527 under reducing conditions for 2ug protein.

Key facts

Purity

>80% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag C-Terminus

Applications

sELISA, SDS-PAGE

applications

Biologically active

No

Accession

O95644

Animal free

Yes

Carrier free

No

Species

Human

Storage buffer

pH: 7.3 - 7.5 Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.

Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.

The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.

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Sequence info

[{"sequence":null,"proteinLength":"Fragment","predictedMolecularWeight":"35.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"O95644","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NFAT2 also known as NFATc1 is a transcription factor with a mass of approximately 100 kDa. It belongs to the Nuclear Factor of Activated T-cells (NFAT) family which plays significant roles in gene expression regulation. NFAT2 is expressed in T-cells B-cells and cardiac muscle tissues. It is activated through dephosphorylation by the enzyme calcineurin allowing it to translocate into the nucleus. NFAT2 binds to specific DNA motifs to regulate the transcription of target genes impacting various cellular processes.
Biological function summary

NFAT2 plays a significant role in the regulation of immune responses and cardiac hypertrophy. It functions as part of a complex with AP-1 (Activator Protein-1) during T-cell activation integrating signals necessary for cytokine production and immune cell differentiation. In cardiac cells NFAT2 influences the expression of genes involved in hypertrophic growth. NFAT2 contributes to the development and function of the immune system by modulating critical immune pathways and cellular proliferation.

Pathways

NFAT2 serves a vital function in the calcineurin-NFAT signaling pathway. This pathway is important for T-cell activation and is triggered by increased intracellular calcium levels leading to dephosphorylation of NFAT2. The dephosphorylated NFAT2 cooperates with proteins such as AP-1 to induce expression of cytokines like interleukin-2. NFAT2 is also linked with the MAPK signaling pathway which affects cellular proliferation and differentiation. Together these pathways highlight the integral role of NFAT2 in immune system function and cardiac development.

NFAT2 has strong connections to autoimmune diseases and cardiac hypertrophy. Aberrations in NFAT2 activity and regulation are associated with autoimmune conditions due to its central role in immune response modulation. In cardiac hypertrophy heightened NFAT2 activity may lead to pathological enlargement of heart tissue. The protein has interactions with calcineurin which also influences these conditions. Understanding these relationships helps in exploring therapeutic targets for managing immune-related diseases and cardiac dysfunctions.
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Specifications

Form

Liquid

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General info

Function

Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells (PubMed : 10358178). Required for osteoclastogenesis and regulates many genes important for osteoclast differentiation and function (By similarity).

Post-translational modifications

Phosphorylated by NFATC-kinase and GSK3B; phosphorylation induces NFATC1 nuclear exit and dephosphorylation by calcineurin promotes nuclear import. Phosphorylation by PKA and DYRK2 negatively modulates nuclear accumulation, and promotes subsequent phosphorylation by GSK3B or casein kinase 1.

Subcellular localisation

Nucleus

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells (PubMed : 10358178). Required for osteoclastogenesis and regulates many genes important for osteoclast differentiation and function (By similarity).
See full target information NFATC1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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