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AB105210

Recombinant human NFkB Inducing Kinase NIK protein

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Recombinant human NFkB Inducing Kinase NIK protein is a Human Fragment protein, in the 325 to 947 aa range, expressed in Baculovirus infected Sf9 cells, with >85%, suitable for SDS-PAGE, WB, FuncS.

View Alternative Names

NIK, MAP3K14, Mitogen-activated protein kinase kinase kinase 14, NF-kappa-beta-inducing kinase, Serine/threonine-protein kinase NIK, HsNIK

4 Images
Functional Studies - Recombinant human NFkB Inducing Kinase NIK protein (AB105210)
  • FuncS

Unknown

Functional Studies - Recombinant human NFkB Inducing Kinase NIK protein (AB105210)

The specific activity of NFkB Inducing Kinase NIK (ab105210) was determined to be 5.5 nmol/min/mg as per activity assay protocol and was equivalent to 7.1 nmol/min/mg as per radiometric assay

Functional Studies - Recombinant human NFkB Inducing Kinase NIK protein (AB105210)
  • FuncS

Unknown

Functional Studies - Recombinant human NFkB Inducing Kinase NIK protein (AB105210)

The Specific activity of ab105210 was determined to be 8 nmol/min/mg.

SDS-PAGE - Recombinant human NFkB Inducing Kinase NIK protein (AB105210)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human NFkB Inducing Kinase NIK protein (AB105210)

SDS PAGE analysis of ab105210

SDS-PAGE - Recombinant human NFkB Inducing Kinase NIK protein (AB105210)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human NFkB Inducing Kinase NIK protein (AB105210)

SDS-PAGE showing ab105210 at approximately 108kDa.

Key facts

Purity

>85% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE, WB, FuncS

applications

Biologically active

Yes

Biological activity

The Specific activity of ab105210 was determined to be 8 nmol/min/mg.

Accession

Q99558

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.307% Glutathione, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

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Complementary Products

Reagents, Controls & Accessories

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Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa) (AB234617)

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Primary Antibodies

AB314146

Anti-NFkB Inducing Kinase NIK antibody [EPR26101-23]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-NFkB Inducing Kinase NIK antibody [EPR26101-23] (AB314146)

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Primary Antibodies

AB223907

Alexa Fluor® 647 Anti-HLA-DR antibody [TAL 1B5]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-HLA-DR antibody [TAL 1B5] (AB223907)

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Primary Antibodies

AB245752

Anti-MAP4K4/NIK antibody [SP177] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-MAP4K4/NIK antibody [SP177] - BSA and Azide free (AB245752)

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Primary Antibodies

AB194319

Alexa Fluor® 647 Anti-VCAM1 antibody [EPR5047]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-VCAM1 antibody [EPR5047] (AB194319)

  • 5
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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Sequence info

[{"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":"108 kDa","actualMolecularWeight":null,"aminoAcidEnd":947,"aminoAcidStart":325,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q99558","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Specifications

Form

Liquid

Additional notes

Purity was determined to be >85% by densitometry. Affinity purified.

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General info

Function

Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Phosphorylates CHUK/IKKA, thereby promoting proteolytic processing of NFKB2/P100, which leads to NF-kappa-B activation via the non-canonical pathway (PubMed : 25406581, PubMed : 29230214). Has an essential role in the non-canonical NF-kappa-B signaling that regulates genes encoding molecules involved in B-cell survival, lymphoid organogenesis, and immune response (PubMed : 25406581). Could act in a receptor-selective manner.

Sequence similarities

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

Post-translational modifications

Autophosphorylated. Phosphorylation at Thr-559 is required to activate its kinase activity and 'Lys-63'-linked polyubiquitination. Phosphorylated by CHUK/IKKA leading to MAP3K14 destabilization.. Ubiquitinated. Undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. 'Lys-48'-linked polyubiquitination leads to its degradation by the proteasome, while 'Lys-63'-linked polyubiquitination stabilizes and activates it.

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Product protocols

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Target data

Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Phosphorylates CHUK/IKKA, thereby promoting proteolytic processing of NFKB2/P100, which leads to NF-kappa-B activation via the non-canonical pathway (PubMed : 25406581, PubMed : 29230214). Has an essential role in the non-canonical NF-kappa-B signaling that regulates genes encoding molecules involved in B-cell survival, lymphoid organogenesis, and immune response (PubMed : 25406581). Could act in a receptor-selective manner.
See full target information MAP3K14
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Product promise

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