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AB179616

Recombinant human NGF protein (Animal Free)

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(4 Publications)

Recombinant human NGF protein (Animal Free) is a Human protein, in the 122 to 241 aa range, expressed in Escherichia coli, with >97%, <1 EU/µg endotoxin level, suitable for SDS-PAGE, HPLC, FuncS.

View Alternative Names

NGFB, NGF, Beta-nerve growth factor, Beta-NGF

Key facts

Purity

>97% SDS-PAGE

Endotoxin level

<1 EU/µg

Expression system

Escherichia coli

Tags

Tag free

Applications

FuncS, SDS-PAGE, HPLC

applications

Biologically active

Yes

Biological activity

The activity is determined by the ability to stimulate proliferation of TF-1 cells and is typically less than 1 ng/mL.

Accession

P01138

Animal free

Yes

Carrier free

No

Species

Human

Reconstitution

Reconstitute at 0.1 mg/mL in water

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"SSSHPIFHRGEFSVCDSVSVWVGDKTTATDIKGKEVMVLGEVNINNSVFKQYFFETKCRDPNPVDSGCRGIDSKHWNSYCTTTHTFVKALTMDGKQAAWRFIRIDTACVCVLSRKAVRRA","proteinLength":null,"predictedMolecularWeight":"14 kDa","actualMolecularWeight":null,"aminoAcidEnd":241,"aminoAcidStart":122,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P01138","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA)
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Specifications

Form

Lyophilized

Additional notes

assessed by HPLC, Reducing and Non-reducing SDS-PAGE, UV spectroscopy at 280 nm. Note: This product is produced with no animal-derived raw products, animal free equipment and animal free protocols.

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General info

Function

Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems (PubMed : 14976160, PubMed : 20978020). Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades to regulate neuronal proliferation, differentiation and survival (Probable) (PubMed : 20978020). The immature NGF precursor (proNGF) functions as a ligand for the heterodimeric receptor formed by SORCS2 and NGFR, and activates cellular signaling cascades that lead to inactivation of RAC1 and/or RAC2, reorganization of the actin cytoskeleton and neuronal growth cone collapse. In contrast to mature NGF, the precursor form (proNGF) promotes neuronal apoptosis (in vitro) (By similarity). Inhibits metalloproteinase-dependent proteolysis of platelet glycoprotein VI (PubMed : 20164177). Binds lysophosphatidylinositol and lysophosphatidylserine between the two chains of the homodimer. The lipid-bound form promotes histamine relase from mast cells, contrary to the lipid-free form (By similarity).

Sequence similarities

Belongs to the NGF-beta family.

Subcellular localisation

Endosome lumen

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Product protocols

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Target data

Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems (PubMed : 14976160, PubMed : 20978020). Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades to regulate neuronal proliferation, differentiation and survival (Probable) (PubMed : 20978020). The immature NGF precursor (proNGF) functions as a ligand for the heterodimeric receptor formed by SORCS2 and NGFR, and activates cellular signaling cascades that lead to inactivation of RAC1 and/or RAC2, reorganization of the actin cytoskeleton and neuronal growth cone collapse. In contrast to mature NGF, the precursor form (proNGF) promotes neuronal apoptosis (in vitro) (By similarity). Inhibits metalloproteinase-dependent proteolysis of platelet glycoprotein VI (PubMed : 20164177). Binds lysophosphatidylinositol and lysophosphatidylserine between the two chains of the homodimer. The lipid-bound form promotes histamine relase from mast cells, contrary to the lipid-free form (By similarity).
See full target information NGF
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Acta naturae 13:69-77 PubMed35127149

2022

The Different Impact of ERK Inhibition on Neuroblastoma, Astrocytoma, and Rhabdomyosarcoma Cell Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

T D Lebedev,E R Vagapova,V S Prassolov

Frontiers in oncology 9:1046 PubMed31681584

2019

Two Receptors, Two Isoforms, Two Cancers: Comprehensive Analysis of KIT and TrkA Expression in Neuroblastoma and Acute Myeloid Leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Timofey D Lebedev,Elmira R Vagapova,Vladimir I Popenko,Olga G Leonova,Pavel V Spirin,Vladimir S Prassolov

Gynecologic oncology 154:228-235 PubMed31003747

2019

Innervation of cervical carcinoma is mediated by cancer-derived exosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher T Lucido,Emily Wynja,Marianna Madeo,Caitlin S Williamson,Lauren E Schwartz,Brittney A Imblum,Ronny Drapkin,Paola D Vermeer

Nature communications 9:4284 PubMed30327461

2018

Cancer exosomes induce tumor innervation.

Applications

Unspecified application

Species

Unspecified reactive species

Marianna Madeo,Paul L Colbert,Daniel W Vermeer,Christopher T Lucido,Jacob T Cain,Elisabeth G Vichaya,Aaron J Grossberg,DesiRae Muirhead,Alex P Rickel,Zhongkui Hong,Jing Zhao,Jill M Weimer,William C Spanos,John H Lee,Robert Dantzer,Paola D Vermeer
View all publications
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Product promise

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