Products
Sample Prep & Detection Kits
Conjugation kitsPurification kitsSample preparation kitsChromogen kitsIHC kitsChIP kitsAccessory Reagents & Controls
Accessory reagents & controlsBiochemicals
BiochemicalsProteins and Peptides
Proteins and peptidesOur latest ELISA kit: Human Tau (phospho T217) - Intracellular
Highly sensitive kit offering the most promising biomarkers for Alzheimer’s disease diagnostics. Learn about all product ranges with our product overviews.
Resources
Featured events
Make new connections at our global events.
Support
Our programs
New Lab Program
Get a head start with our exclusive new lab discount. Enjoy 20% off and free shipping for three months.
New Biotech Program
Just starting out? Get 15% off and free shipping to your lab for six months.
Product promise
Peace of mind that all products perform as stated.
Product reviews
Leave reviews, get rewarded and help your community.
Trial program
Try untested species and applications to earn money off your next order.
Recombinant human NGF protein (Animal Free) is a Human Fragment protein, in the 122 to 239 aa range, expressed in Escherichia coli, with >98% purity and suitable for SDS-PAGE, FuncS, HPLC.
Alternative names=Beta-nerve growth factor, Beta-NGF, NGFB, NGF
- Purity
>98% SDS-PAGE
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
SDS-PAGE, FuncS, HPLC
- Biologically active
Yes
- Sequence
S S S H P I F H R G E F S V C D S V S V W V G D K T T A T D I K G K E V M V L G E V N I N N S V F K Q Y F F E T K C R D P N P V D S G C R G I D S K H W N S Y C T T T H T F V K A L T M D G K Q A A W R F I R I D T A C V C V L S R K A V R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Recommended products
Recombinant human NGF protein (Animal Free) is a Human Fragment protein, in the 122 to 239 aa range, expressed in Escherichia coli, with >98% purity and suitable for SDS-PAGE, FuncS, HPLC.
Alternative names=Beta-nerve growth factor, Beta-NGF, NGFB, NGF
Key facts
- Purity
>98% SDS-PAGE
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
SDS-PAGE, FuncS, HPLC
- Biologically active
Yes
- Biological activity
Determined by the dose-dependent stimulation of the proliferation of human TF-1 cells. The expected ED50 is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.
- Predicted molecular weight
13.27 kDa
- Amino acids
122 to 239
- Sequence
S S S H P I F H R G E F S V C D S V S V W V G D K T T A T D I K G K E V M V L G E V N I N N S V F K Q Y F F E T K C R D P N P V D S G C R G I D S K H W N S Y C T T T H T F V K A L T M D G K Q A A W R F I R I D T A C V C V L S R K A V R
- Accession
- P01138-1
- Protein length
Fragment
- Animal free
Yes
- Nature
Recombinant
- Species
Human
- Concentration
Specifications
- Form
Lyophilized
- Additional notes
> 98 % by HPLC.
General info
- Function
Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems (PubMed:14976160, PubMed:20978020). Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades to regulate neuronal proliferation, differentiation and survival (PubMed:20978020) (Probable). The immature NGF precursor (proNGF) functions as ligand for the heterodimeric receptor formed by SORCS2 and NGFR, and activates cellular signaling cascades that lead to inactivation of RAC1 and/or RAC2, reorganization of the actin cytoskeleton and neuronal growth cone collapse. In contrast to mature NGF, the precursor form (proNGF) promotes neuronal apoptosis (in vitro) (By similarity). Inhibits metalloproteinase-dependent proteolysis of platelet glycoprotein VI (PubMed:20164177). Binds lysophosphatidylinositol and lysophosphatidylserine between the two chains of the homodimer. The lipid-bound form promotes histamine relase from mast cells, contrary to the lipid-free form (By similarity).
- Sequence similarities
Belongs to the NGF-beta family.
- Subcellular localisation
Endosome lumen
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com