Recombinant Human Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 protein (His tag) is a Human Fragment protein, in the 1 to 254 aa range, expressed in HEK 293, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M A A R G S G P R A L R L L L L V Q L V A G R C G L A G A A G G A Q R G L S E P S S I A K H E D S L L K D L F Q D Y E R W V R P V E H L N D K I K I K F G L A I S Q L V D V D E K N Q L M T T N V W L K Q E W I D V K L R W N P D D Y G G I K V I R V P S D S V W T P D I V L F D N A D G R F E G T S T K T V I R Y N G T V T W T P P A N Y K S S C T I D V T F F P F D L Q N C S M K F G S W T Y D G S Q V D I I L E D Q D V D K R D F F D N G E W E I V S A T G S K G N R T D S C C W Y P Y V T Y S F V I K R L P L F Y T
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
Alternative names
NACHRA5, CHRNA5, Neuronal acetylcholine receptor subunit alpha-5
Recommended products
Recombinant Human Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 protein (His tag) is a Human Fragment protein, in the 1 to 254 aa range, expressed in HEK 293, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P30532-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- M A A R G S G P R A L R L L L L V Q L V A G R C G L A G A A G G A Q R G L S E P S S I A K H E D S L L K D L F Q D Y E R W V R P V E H L N D K I K I K F G L A I S Q L V D V D E K N Q L M T T N V W L K Q E W I D V K L R W N P D D Y G G I K V I R V P S D S V W T P D I V L F D N A D G R F E G T S T K T V I R Y N G T V T W T P P A N Y K S S C T I D V T F F P F D L Q N C S M K F G S W T Y D G S Q V D I I L E D Q D V D K R D F F D N G E W E I V S A T G S K G N R T D S C C W Y P Y V T Y S F V I K R L P L F Y T
- Accession
- P30532
- Protein length
- Fragment
- Predicted molecular weight
- 28 kDa
- Amino acids
- 1 to 254
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
- Sequence similarities
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-5/CHRNA5 sub-subfamily.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Nicotinic Acetylcholine Receptor alpha 5 (CHRNA5) also known as the nicotinic receptor plays a mechanical role as part of the heteromeric nicotinic acetylcholine receptor (nAChR) complex. It is a subunit that contributes to the formation of ligand-gated ion channels sensitive to acetylcholine a neurotransmitter. The receptor's mass varies depending on the specific subunit composition but is generally around 55 kDa. CHRNA5 expression occurs mainly in the central nervous system including areas like the brain and spinal cord and it also shows expression in some peripheral tissues.
- Biological function summary
The Nicotinic Acetylcholine Receptor alpha 5 modulates neural signaling by affecting the flow of ions through its channel which is activated upon acetylcholine binding. It is part of a complex that includes other nicotinic acetylcholine receptor subunits forming a transmembrane channel. This receptor complex adjusts neurotransmission dynamics and influences synaptic plasticity. It plays roles in cognitive processes and behavioral regulation through its modulation of cholinergic signaling pathways.
- Pathways
The Nicotinic Acetylcholine Receptor alpha 5 influences neural signaling through involvement in the cholinergic and dopamine pathways. It interacts with proteins like CHRNA4 and CHRNB2 integral components of these pathways where it modulates synaptic activity and neurotransmission. The receptor's function impacts neurotransmitter release which is critical for processing cognitive and reward-related signals in the brain.
- Associated diseases and disorders
The Nicotinic Acetylcholine Receptor alpha 5 has links to nicotine addiction and schizophrenia. Genetic variations in CHRNA5 are associated with increased risk for nicotine dependence suggesting its role in addiction pathways. Studies implicate it in schizophrenia where its altered expression or function might affect dopaminergic signaling balance. The receptor's association with proteins like CHRNA4 highlights its involvement in disorders through complex interactions in neural transmission.
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SDS-PAGE - Recombinant Human Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 protein (His tag) (ab276630)
SDS-PAGE analysis of ab276630
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