Recombinant Human Nidogen 2/NID-2 protein is a Human Full Length protein, in the 1 to 969 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M E G D R V A G R P V L S S L P V L L L L Q L L M L R A A A L H P D E L F P H G E S W G D Q L L Q E G D D E S S A V V K L A N P L H F Y E A R F S N L Y V G T N G I I S T Q D F P R E T Q Y V D Y D F P T D F P A I A P F L A D I D T S H G R G R V L Y R V N G K V S G H L H V G H T P V H F T D V D L H A Y I V G N D G R A Y T A I S H I P Q P A A Q A L L P L T P I G G L F G W L F A L E K P G S E N G F S L A G A A F T H D M E V T F Y P G E E T V R I T Q T A E G L D P E N Y L S I K T N I Q G Q V P Y V P A N F T A H I S P Y K E L Y H Y S D S T V T S T S S R D Y S L T F G A I N Q T W S Y R I H Q N I T Y Q V C R H A P R H P S F P T T Q Q L N V D R V F A L Y N D E E R V L R F A V T N Q I G P V K E D S D P T P V N P C Y D G S H M C D T T A R C H P G T G V D Y T C E C A S G Y Q G D G R N C V D E N E C A T G F H R C G P N S V C I N L P G S Y R C E C R S A Y E F A D D R H T C I L I T P P A N P C E D G S H T C A P A G Q A R C V H H G G S T F S C A C L P G Y A G D G H Q C T D V D E C S E N R C H P A A T C Y N T P G S F S C R C Q P G Y Y G D G F Q C I P D S T S S L T P C E Q Q Q R H A Q A Q Y A Y P G A R F H I P Q C D E Q G N F L P L Q C H G S T G F C W C V D P D G H E V P G T Q T P P G S T P P H C G P S P E P T Q R P P T I C E R W R E N L L E H Y G G T P R D D Q Y V P Q C D D L G H F I P L Q C H G K S D F C W C V D K D G R E V Q G T R S Q P G T T P A C I P T V A P P M V R P T P R P D V T P P S V G T F L L Y T Q G Q Q I G Y L P L N G T R L Q K D A A K T L L S L H G S I I V G I D Y D C R E R M V Y W T D V A G R T I S R A G L E L G A E P E T I V N S G L I S P E G L A I D H I R R T M Y W T D S V L D K I E S A L L D G S E R K V L F Y T D L V N P R A I A V D P I R G N L Y W T D W N R E A P K I E T S S L D G E N R R I L I N T D I G L P N G L T F D P F S K L L C W A D A G T K K L E C T L P D G T G R R V I Q N N L K Y P F S I V S Y A D H F Y H T D W R R D G V V S V N K H S G Q F T D E Y L P E Q R S H L Y G I T A V Y P Y C P T G R K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cell adhesion glycoprotein which is widely distributed in basement membranes. Binds to collagens I and IV, to perlecan and to laminin 1. Does not bind fibulins. It probably has a role in cell-extracellular matrix interactions.
Alternative names
Nidogen-2, NID-2, Osteonidogen, NID2
Recommended products
Recombinant Human Nidogen 2/NID-2 protein is a Human Full Length protein, in the 1 to 969 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- Q14112-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M E G D R V A G R P V L S S L P V L L L L Q L L M L R A A A L H P D E L F P H G E S W G D Q L L Q E G D D E S S A V V K L A N P L H F Y E A R F S N L Y V G T N G I I S T Q D F P R E T Q Y V D Y D F P T D F P A I A P F L A D I D T S H G R G R V L Y R V N G K V S G H L H V G H T P V H F T D V D L H A Y I V G N D G R A Y T A I S H I P Q P A A Q A L L P L T P I G G L F G W L F A L E K P G S E N G F S L A G A A F T H D M E V T F Y P G E E T V R I T Q T A E G L D P E N Y L S I K T N I Q G Q V P Y V P A N F T A H I S P Y K E L Y H Y S D S T V T S T S S R D Y S L T F G A I N Q T W S Y R I H Q N I T Y Q V C R H A P R H P S F P T T Q Q L N V D R V F A L Y N D E E R V L R F A V T N Q I G P V K E D S D P T P V N P C Y D G S H M C D T T A R C H P G T G V D Y T C E C A S G Y Q G D G R N C V D E N E C A T G F H R C G P N S V C I N L P G S Y R C E C R S A Y E F A D D R H T C I L I T P P A N P C E D G S H T C A P A G Q A R C V H H G G S T F S C A C L P G Y A G D G H Q C T D V D E C S E N R C H P A A T C Y N T P G S F S C R C Q P G Y Y G D G F Q C I P D S T S S L T P C E Q Q Q R H A Q A Q Y A Y P G A R F H I P Q C D E Q G N F L P L Q C H G S T G F C W C V D P D G H E V P G T Q T P P G S T P P H C G P S P E P T Q R P P T I C E R W R E N L L E H Y G G T P R D D Q Y V P Q C D D L G H F I P L Q C H G K S D F C W C V D K D G R E V Q G T R S Q P G T T P A C I P T V A P P M V R P T P R P D V T P P S V G T F L L Y T Q G Q Q I G Y L P L N G T R L Q K D A A K T L L S L H G S I I V G I D Y D C R E R M V Y W T D V A G R T I S R A G L E L G A E P E T I V N S G L I S P E G L A I D H I R R T M Y W T D S V L D K I E S A L L D G S E R K V L F Y T D L V N P R A I A V D P I R G N L Y W T D W N R E A P K I E T S S L D G E N R R I L I N T D I G L P N G L T F D P F S K L L C W A D A G T K K L E C T L P D G T G R R V I Q N N L K Y P F S I V S Y A D H F Y H T D W R R D G V V S V N K H S G Q F T D E Y L P E Q R S H L Y G I T A V Y P Y C P T G R K
- Accession
- Q14112
- Protein length
- Full Length
- Amino acids
- 1 to 969
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Cell adhesion glycoprotein which is widely distributed in basement membranes. Binds to collagens I and IV, to perlecan and to laminin 1. Does not bind fibulins. It probably has a role in cell-extracellular matrix interactions.
- Post-translational modifications
Highly N-glycosylated.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as Nidogen 2.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
'Nidogen 2' also called 'NID-2' or '"nidogen protein'" or '"nid-2"' is a glycoprotein found in basement membranes. It has a molecular mass around 150 kDa and expresses across various tissues particularly in the vascular structures and certain epithelial tissues. This protein has sulfated and carbohydrate-rich regions making it important in forming stable extracellular matrix architecture.
- Biological function summary
'nidogen 2' plays roles in maintaining the structural integrity of basement membranes. It interacts with other matrix components like laminin and collagen IV forming part of complexes that ensure the basal membrane's maintenance and stability. 'NID-2' provides structural support and helps secure cellular environments which is important for tissue repair and morphogenesis.
- Pathways
'nidogen 2' contributes to the extracellular matrix remodeling and cell signaling pathways. It participates in the integrin signaling pathway where it interacts with proteins such as 'integrin' and 'perlecan'. These interactions help mediate cellular adhesion and signal transduction impacting cellular behavior like proliferation differentiation and migration processes.
- Associated diseases and disorders
Abnormalities in 'nidogen 2' relate to certain cancers and kidney disorders. In cancer disrupted interactions with 'laminin' may promote tumor cell invasion and metastasis. In kidney disorders such as nephrotic syndrome altered 'nidogen 2' function relates to basement membrane defects affecting kidney filtration function. Understanding its role could lead to insights into therapies for these conditions.
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1 product image
SDS-PAGE - Recombinant Human Nidogen 2/NID-2 protein (ab153185)
ab153185 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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