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AB131913

Recombinant Human NIPBL protein

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(1 Publication)

Recombinant Human NIPBL protein is a Human Fragment protein, in the 2524 to 2697 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

IDN3, SCC2, NIPBL, Nipped-B-like protein, Delangin, SCC2 homolog

1 Images
SDS-PAGE - Recombinant Human NIPBL protein (AB131913)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human NIPBL protein (AB131913)

ab131913 on a 12.5% SDS-PAGE Stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

Tag free

Applications

SDS-PAGE, WB, ELISA

applications

Biologically active

No

Accession

Q6KC79

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as IDN3.
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Sequence info

[{"sequence":"MKCLPENSAPLIEFANVSQGILLLLMLKQHLKNLCGFSDSKIQKYSPSESAKVYDKAINRKTGVHFHPKQTLDFLRSDMANSKITEEVKRSIVKQYLDFKLLMEHLDPDEEEEEGEVSASTNARNKAITSLLGGGSPKNNTAAETEDDESDGEDRGGGTSGVRRRRSQRISQRIT","proteinLength":"Fragment","predictedMolecularWeight":"45.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":2697,"aminoAcidStart":2524,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q6KC79","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

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General info

Function

Plays an important role in the loading of the cohesin complex on to DNA. Forms a heterodimeric complex (also known as cohesin loading complex) with MAU2/SCC4 which mediates the loading of the cohesin complex onto chromatin (PubMed : 22628566, PubMed : 28914604). Plays a role in cohesin loading at sites of DNA damage. Its recruitment to double-strand breaks (DSBs) sites occurs in a CBX3-, RNF8- and RNF168-dependent manner whereas its recruitment to UV irradiation-induced DNA damage sites occurs in a ATM-, ATR-, RNF8- and RNF168-dependent manner (PubMed : 28167679). Along with ZNF609, promotes cortical neuron migration during brain development by regulating the transcription of crucial genes in this process. Preferentially binds promoters containing paused RNA polymerase II. Up-regulates the expression of SEMA3A, NRP1, PLXND1 and GABBR2 genes, among others (By similarity).

Sequence similarities

Belongs to the SCC2/Nipped-B family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Plays an important role in the loading of the cohesin complex on to DNA. Forms a heterodimeric complex (also known as cohesin loading complex) with MAU2/SCC4 which mediates the loading of the cohesin complex onto chromatin (PubMed : 22628566, PubMed : 28914604). Plays a role in cohesin loading at sites of DNA damage. Its recruitment to double-strand breaks (DSBs) sites occurs in a CBX3-, RNF8- and RNF168-dependent manner whereas its recruitment to UV irradiation-induced DNA damage sites occurs in a ATM-, ATR-, RNF8- and RNF168-dependent manner (PubMed : 28167679). Along with ZNF609, promotes cortical neuron migration during brain development by regulating the transcription of crucial genes in this process. Preferentially binds promoters containing paused RNA polymerase II. Up-regulates the expression of SEMA3A, NRP1, PLXND1 and GABBR2 genes, among others (By similarity).
See full target information NIPBL
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Human molecular genetics 22:4180-93 PubMed23760082

2013

Regional chromatin decompaction in Cornelia de Lange syndrome associated with NIPBL disruption can be uncoupled from cohesin and CTCF.

Applications

WB

Species

Unspecified reactive species

Leisha D Nolen,Shelagh Boyle,Morad Ansari,Emily Pritchard,Wendy A Bickmore
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Complementary Products

Primary Antibodies

AB106768

Anti-NIPBL antibody [KT55]

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Primary Antibodies

AB183033

Anti-Scc4 antibody [EPR14390]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Scc4 antibody [EPR14390] (AB183033)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB317301

Anti-SA1 antibody [EPR26227-76]

20ul selling size|Recombinant|Advanced Validation|RabMAb|Trial Size

Flow Cytometry (Intracellular) - Anti-SA1 antibody [EPR26227-76] (AB317301)

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