Recombinant Human NKIAMRE protein is a Human Full Length protein, in the 1 to 592 aa range, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, WB.
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Key facts
- Purity
- >70% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, WB
- Biologically active
- No
Amino acid sequence
M E M Y E T L G K V G E G S Y G T V M K C K H K N T G Q I V A I K I F Y E R P E Q S V N K I A M R E I K F L K Q F H H E N L V N L I E V F R Q K K K I H L V F E F I D H T V L D E L Q H Y C H G L E S K R L R K Y L F Q I L R A I D Y L H S N N I I H R D I K P E N I L V S Q S G I T K L C D F G F A R T L A A P G D I Y T D Y V A T R W Y R A P E L V L K D T S Y G K P V D I W A L G C M I I E M A T G N P Y L P S S S D L D L L H K I V L K V G N L S P H L Q N I F S K S P I F A G V V L P Q V Q H P K N A R K K Y P K L N G L L A D I V H A C L Q I D P A D R I S S S D L L H H E Y F T R D G F I E K F M P E L K A K L L Q E A K V N S L I K P K E S S K E N E L R K D E R K T V Y T N T L L S S S V L G K E I E K E K K P K E I K V R V I K V K G G R G D I S E P K K K E Y E G G L G Q Q D A N E N V H P M S P D T K L V T I E P P N P I N P S T N C N G L K E N P H C G G S V T M P P I N L T N S N L M A A N L S S N L F H P S V R L T E R A K K R R T S S Q S I G Q V M P N S R Q E D P G P I Q S Q M E K G I F N E R T G H S D Q M A N E N K R K L N F S R S D R K E F H F P E L P V T I Q S K D T K G M E V K Q I K M L K R E S K K T E S S K I P T L L N V D Q N Q E K Q E G G D G H C E G K N L K R N R F F F W
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Alternative names
NKIAMRE, CDKL3, Cyclin-dependent kinase-like 3, Serine/threonine-protein kinase NKIAMRE
Recommended products
Recombinant Human NKIAMRE protein is a Human Full Length protein, in the 1 to 592 aa range, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, WB.
Key facts
- Purity
- >70% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, WB
- Biologically active
- No
- Accession
- Q8IVW4-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.79% Tris HCl, 0.31% Glutathione, 0.29% Sodium chloride, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- M E M Y E T L G K V G E G S Y G T V M K C K H K N T G Q I V A I K I F Y E R P E Q S V N K I A M R E I K F L K Q F H H E N L V N L I E V F R Q K K K I H L V F E F I D H T V L D E L Q H Y C H G L E S K R L R K Y L F Q I L R A I D Y L H S N N I I H R D I K P E N I L V S Q S G I T K L C D F G F A R T L A A P G D I Y T D Y V A T R W Y R A P E L V L K D T S Y G K P V D I W A L G C M I I E M A T G N P Y L P S S S D L D L L H K I V L K V G N L S P H L Q N I F S K S P I F A G V V L P Q V Q H P K N A R K K Y P K L N G L L A D I V H A C L Q I D P A D R I S S S D L L H H E Y F T R D G F I E K F M P E L K A K L L Q E A K V N S L I K P K E S S K E N E L R K D E R K T V Y T N T L L S S S V L G K E I E K E K K P K E I K V R V I K V K G G R G D I S E P K K K E Y E G G L G Q Q D A N E N V H P M S P D T K L V T I E P P N P I N P S T N C N G L K E N P H C G G S V T M P P I N L T N S N L M A A N L S S N L F H P S V R L T E R A K K R R T S S Q S I G Q V M P N S R Q E D P G P I Q S Q M E K G I F N E R T G H S D Q M A N E N K R K L N F S R S D R K E F H F P E L P V T I Q S K D T K G M E V K Q I K M L K R E S K K T E S S K I P T L L N V D Q N Q E K Q E G G D G H C E G K N L K R N R F F F W
- Accession
- Q8IVW4
- Protein length
- Full Length
- Predicted molecular weight
- 68 kDa
- Amino acids
- 1 to 592
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NKIAMRE also known as Cyclin G-associated kinase (GAK) is a protein involved in cellular processes such as regulation of the cell cycle and signal transduction. It has an approximate mass of 148 kDa. NKIAMRE is expressed in various tissues including the brain heart and kidneys. This protein has kinase activity which allows it to phosphorylate other proteins influencing their function and activity in cells.
- Biological function summary
NKIAMRE modulates cell growth and differentiation. While not identified as part of a specific complex it interacts with several proteins implicating it in cellular signaling networks. These interactions suggest it has roles in managing intracellular signaling pathways affecting how cells respond to various external and internal signals. It is especially relevant in maintaining the normal functioning of cells.
- Pathways
NKIAMRE interacts closely with cell cycle and signal transduction pathways. It participates directly in the regulation of the cell cycle where it affects the progression of cells through different phases of cell division. Cyclin-dependent kinases (CDKs) function alongside NKIAMRE in this regard coordinating cell cycle regulation. Additionally NKIAMRE's involvement in signal transduction connects it to the MAPK signaling pathway an important route in conveying extracellular signals to the cellular machinery.
- Associated diseases and disorders
NKIAMRE links closely with certain cancers and neurodegenerative diseases. Abnormal expression of this protein correlates with the progression of some cancer types where it affects cell proliferation and survival. Also its role in the brain environment highlights relevance in conditions like Alzheimer's disease. Aberrant interactions between NKIAMRE and tau protein a major factor in Alzheimer's suggest a significant link with disease pathogenesis. Understanding NKIAMRE's functions and interactions could unravel more about its role in these diseases.
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SDS-PAGE - Recombinant Human NKIAMRE protein (ab172189)
SDS-PAGE analysis of ab172189.
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