Recombinant human NKp30 protein (Fc Chimera Active) is a Human Fragment protein, in the 19 to 135 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, ELISA.
Images
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus
- Applications
- SDS-PAGE, ELISA
- Biologically active
- Yes
Amino acid sequence
L W V S Q P P E I R T L E G S S A F L P C S F N A S Q G R L A I G S V T W F R D E V V P G K E V R N G T P E F R G R L A P L A S S R F L H D H Q A E L H I R D V R G H D A S I Y V C R V E V L G L G V G T G N G T R L V V E K E H P Q L G
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cell membrane receptor of natural killer/NK cells that is activated by binding of extracellular ligands including BAG6 and NCR3LG1. Stimulates NK cells cytotoxicity toward neighboring cells producing these ligands. It controls, for instance, NK cells cytotoxicity against tumor cells. Engagement of NCR3 by BAG6 also promotes myeloid dendritic cells (DC) maturation, both through killing DCs that did not acquire a mature phenotype, and inducing the release by NK cells of TNFA and IFNG which promote DC maturation.
Alternative names
CD337, 1C7, LY117, NCR3, Natural cytotoxicity triggering receptor 3, Activating natural killer receptor p30, Natural killer cell p30-related protein, NK-p30, NKp30
Recommended products
Recombinant human NKp30 protein (Fc Chimera Active) is a Human Fragment protein, in the 19 to 135 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, ELISA.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus
- Applications
- SDS-PAGE, ELISA
- Biologically active
- Yes
- Biological activity
- Measured by its binding ability in a functional ELISA. Immobilized Human B7-H6, His Tag at 10 μg/mL (100 μL/well) can bind ab184896 with a linear range of 2-78 ng/mL.
- Accession
- O14931-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute at 400 µg/mL in water
- Concentration
- Storage buffer
pH: 7.4
Constituents: 5% Trehalose, 0.75% Glycine, 0.605% Tris
Sequence info
Amino acid sequence
- L W V S Q P P E I R T L E G S S A F L P C S F N A S Q G R L A I G S V T W F R D E V V P G K E V R N G T P E F R G R L A P L A S S R F L H D H Q A E L H I R D V R G H D A S I Y V C R V E V L G L G V G T G N G T R L V V E K E H P Q L G
- Accession
- O14931
- Protein length
- Fragment
- Predicted molecular weight
- 39.5 kDa
- Amino acids
- 19 to 135
- Nature
- Recombinant
- Tags
- Fc tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Cell membrane receptor of natural killer/NK cells that is activated by binding of extracellular ligands including BAG6 and NCR3LG1. Stimulates NK cells cytotoxicity toward neighboring cells producing these ligands. It controls, for instance, NK cells cytotoxicity against tumor cells. Engagement of NCR3 by BAG6 also promotes myeloid dendritic cells (DC) maturation, both through killing DCs that did not acquire a mature phenotype, and inducing the release by NK cells of TNFA and IFNG which promote DC maturation.
- Sequence similarities
Belongs to the natural cytotoxicity receptor (NCR) family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Reconstitute for long term storage
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NKp30 also known as NCR3 (Natural Cytotoxicity Receptor 3) is a type I transmembrane protein that functions as an activating receptor on natural killer (NK) cells. It is part of the natural cytotoxicity receptor family and is encoded by the NCR3 gene. NKp30 is characterized by its structure which includes a single immunoglobulin-like domain. This protein weighs approximately 30 kDa. NKp30 is expressed mainly on resting and activated NK cells as well as IL-15-stimulated NK cells found in humans.
- Biological function summary
NKp30 plays a significant role in immune response by recognizing and binding to ligands on tumor cells and virus-infected cells facilitating their elimination. It forms part of a larger immunological complex that includes DNAM-1 and 2B4 other activating receptors that work in concert to enhance the natural killing ability of NK cells. NKp30 triggers the release of cytotoxic granules and cytokines like IFN-γ leading to an enhanced cytotoxic response against target cells.
- Pathways
NKp30 participates heavily in immune regulation especially in NK cell-mediated cytotoxicity. This protein is involved in pathways such as the NK cell-mediated antibody-dependent cellular cytotoxicity (ADCC) and has strong interaction with signaling proteins like CD3ζ and DAP12 which transduce activating signals. Through these pathways NKp30 influences the immune surveillance capability of NK cells modulating immune responses and maintaining cellular homeostasis.
- Associated diseases and disorders
NKp30 has been implicated in cancer progression and immune evasion notably in melanoma and gastrointestinal stromal tumors (GIST). Altered expression of NKp30 can result in reduced immune recognition and elimination of these tumor cells. The protein's interaction with B7-H6 a ligand often overexpressed on cancer cells illustrates its importance in tumor immunity. NKp30's function or dysfunction makes it a relevant target for therapeutic interventions aimed at restoring immune competence against cancerous cells.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
ELISA - Recombinant human NKp30 protein (Fc Chimera Active) (ab184896)
Immobilized Human B7-H6, His Tag at 10 μg/mL (100 μL/well) can bind ab184896 with a linear range of 2-78 ng/mL.
SDS-PAGE - Recombinant human NKp30 protein (Fc Chimera Active) (ab184896)
SDS-PAGE of reduced ab184896 stained overnight with Coomassie Blue. The protein migrates as 50-58 kDa due to glycosylation.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com