Recombinant Human NKRF protein is a Human Full Length protein, in the 1 to 690 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M E K I L Q M A E G I D I G E M P S Y D L V L S K P S K G Q K R H L S T C D G Q N P P K K Q A G S K F H A R P R F E P V H F V A S S S K D E R Q E D P Y G P Q T K E V N E Q T H F A S M P R D I Y Q D Y T Q D S F S I Q D G N S Q Y C D S S G F I L T K D Q P V T A N M Y F D S G N P A P S T T S Q Q A N S Q S T P E P S P S Q T F P E S V V A E K Q Y F I E K L T A T I W K N L S N P E M T S G S D K I N Y T Y M L T R C I Q A C K T N P E Y I Y A P L K E I P P A D I P K N K K L L T D G Y A C E V R C Q N I Y L T T G Y A G S K N G S R D R A T E L A V K L L Q K R I E V R V V R R K F K H T F G E D L V V C Q I G M S S Y E F P P A L K Q P E D L V V L G K D A S G Q P I F N A S A K H W T N F V I T E N A N D A I G I L N N S A S F N K M S I E Y K Y E M M P N R T W R C R V F L Q D H C L A E G Y G T K K T S K H A A A D E A L K I L Q K T Q P T Y P S V K S S Q C H T G S S P R G S G K K K D I K D L V V Y E N S S N P V C T L N D T A Q F N R M T V E Y V Y E R M T G L R W K C K V I L E S E V I A E A V G V K K T V K Y E A A G E A V K T L K K T Q P T V I N N L K K G A V E D V I S R N E I Q G R S A E E A Y K Q Q I K E D N I G N Q L L R K M G W T G G G L G K S G E G I R E P I S V K E Q H K R E G L G L D V E R V N K I A K R D I E Q I I R N Y A R S E S H T D L T F S R E L T N D E R K Q I H Q I A Q K Y G L K S K S H G V G H D R Y L V V G R K R R K E D L L D Q L K Q E G Q V G H Y E L V M P Q A N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Enhances the ATPase activity of DHX15 by acting like a brace that tethers mobile sections of DHX15 together, stabilizing a functional conformation with high RNA affinity of DHX15 (PubMed:12381793). Involved in the constitutive silencing of the interferon beta promoter, independently of the virus-induced signals, and in the inhibition of the basal and cytokine-induced iNOS promoter activity (PubMed:12381793). Also involved in the regulation of IL-8 transcription (PubMed:12381793). May also act as a DNA-binding transcription regulator: interacts with a specific negative regulatory element (NRE) 5'-AATTCCTCTGA-3' to mediate transcriptional repression of certain NK-kappa-B responsive genes (PubMed:10562553).
Alternative names
ITBA4, NRF, NKRF, NF-kappa-B-repressing factor, NFkB-repressing factor, Protein ITBA4
Recommended products
Recombinant Human NKRF protein is a Human Full Length protein, in the 1 to 690 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- O15226-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M E K I L Q M A E G I D I G E M P S Y D L V L S K P S K G Q K R H L S T C D G Q N P P K K Q A G S K F H A R P R F E P V H F V A S S S K D E R Q E D P Y G P Q T K E V N E Q T H F A S M P R D I Y Q D Y T Q D S F S I Q D G N S Q Y C D S S G F I L T K D Q P V T A N M Y F D S G N P A P S T T S Q Q A N S Q S T P E P S P S Q T F P E S V V A E K Q Y F I E K L T A T I W K N L S N P E M T S G S D K I N Y T Y M L T R C I Q A C K T N P E Y I Y A P L K E I P P A D I P K N K K L L T D G Y A C E V R C Q N I Y L T T G Y A G S K N G S R D R A T E L A V K L L Q K R I E V R V V R R K F K H T F G E D L V V C Q I G M S S Y E F P P A L K Q P E D L V V L G K D A S G Q P I F N A S A K H W T N F V I T E N A N D A I G I L N N S A S F N K M S I E Y K Y E M M P N R T W R C R V F L Q D H C L A E G Y G T K K T S K H A A A D E A L K I L Q K T Q P T Y P S V K S S Q C H T G S S P R G S G K K K D I K D L V V Y E N S S N P V C T L N D T A Q F N R M T V E Y V Y E R M T G L R W K C K V I L E S E V I A E A V G V K K T V K Y E A A G E A V K T L K K T Q P T V I N N L K K G A V E D V I S R N E I Q G R S A E E A Y K Q Q I K E D N I G N Q L L R K M G W T G G G L G K S G E G I R E P I S V K E Q H K R E G L G L D V E R V N K I A K R D I E Q I I R N Y A R S E S H T D L T F S R E L T N D E R K Q I H Q I A Q K Y G L K S K S H G V G H D R Y L V V G R K R R K E D L L D Q L K Q E G Q V G H Y E L V M P Q A N
- Accession
- O15226
- Protein length
- Full Length
- Predicted molecular weight
- 104.1 kDa
- Amino acids
- 1 to 690
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
Enhances the ATPase activity of DHX15 by acting like a brace that tethers mobile sections of DHX15 together, stabilizing a functional conformation with high RNA affinity of DHX15 (PubMed:12381793). Involved in the constitutive silencing of the interferon beta promoter, independently of the virus-induced signals, and in the inhibition of the basal and cytokine-induced iNOS promoter activity (PubMed:12381793). Also involved in the regulation of IL-8 transcription (PubMed:12381793). May also act as a DNA-binding transcription regulator: interacts with a specific negative regulatory element (NRE) 5'-AATTCCTCTGA-3' to mediate transcriptional repression of certain NK-kappa-B responsive genes (PubMed:10562553).
- Subcellular localisation
- Nucleus, Nucleolus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NKRF also known as NF-kappa-B repressing factor functions as a transcriptional repressor. This protein weighs approximately 85 kDa and appears in various tissues with higher expression in the spleen thymus and lungs. NKRF interacts with NF-kB elements in DNA inhibiting the transcription of specific genes especially those related to immune responses and inflammation.
- Biological function summary
NKRF plays an important role in modulating inflammation and immune responses. It forms part of a complex that controls the transcriptional activity of NF-kappa-B an important transcription factor in these processes. By repressing unwanted NF-kappa-B signal NKRF prevents excessive immune response and inflammation contributing to cellular homeostasis and protecting tissues from damage.
- Pathways
NKRF functions prominently in the NF-kappa-B signaling and inflammatory pathways. Within these pathways it regulates the activity of related proteins such as RelA/p65 which are components of the NF-kappa-B family. NKRF balances the inflammation by downregulating the genes activated by NF-kB making sure the response stays controlled.
- Associated diseases and disorders
NKRF is critical in conditions associated with chronic inflammation. High or low levels of NKRF correlate with diseases such as asthma and rheumatoid arthritis. In asthma NKRF may modulate the activity of pro-inflammatory cytokines while in rheumatoid arthritis it may interact with upstream molecules like IKK complex to control inflammation.
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1 product image
SDS-PAGE - Recombinant Human NKRF protein (ab132717)
ab132717 analyzed by 12.5% SDS-PAGE stained with Coomassie Blue.
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