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AB132717

Recombinant Human NKRF protein (GST tag N-Terminus)

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Recombinant Human NKRF protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 690 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

ITBA4, NRF, NKRF, NF-kappa-B-repressing factor, NFkB-repressing factor, Protein ITBA4

1 Images
SDS-PAGE - Recombinant Human NKRF protein (GST tag N-Terminus) (AB132717)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human NKRF protein (GST tag N-Terminus) (AB132717)

ab132717 analyzed by 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA, SDS-PAGE

applications

Biologically active

No

Accession

O15226

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

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Primary Antibodies

AB168829

Anti-NKRF antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKRF antibody (AB168829)

  • 0
  • 0
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB179467

Anti-Actin antibody [EPR16769] - Loading Control

RabMAb|Recombinant|Lab Essentials

Flow Cytometry (Intracellular) - Anti-Actin antibody [EPR16769] - Loading Control (AB179467)

  • 5
  • 11
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MEKILQMAEGIDIGEMPSYDLVLSKPSKGQKRHLSTCDGQNPPKKQAGSKFHARPRFEPVHFVASSSKDERQEDPYGPQTKEVNEQTHFASMPRDIYQDYTQDSFSIQDGNSQYCDSSGFILTKDQPVTANMYFDSGNPAPSTTSQQANSQSTPEPSPSQTFPESVVAEKQYFIEKLTATIWKNLSNPEMTSGSDKINYTYMLTRCIQACKTNPEYIYAPLKEIPPADIPKNKKLLTDGYACEVRCQNIYLTTGYAGSKNGSRDRATELAVKLLQKRIEVRVVRRKFKHTFGEDLVVCQIGMSSYEFPPALKQPEDLVVLGKDASGQPIFNASAKHWTNFVITENANDAIGILNNSASFNKMSIEYKYEMMPNRTWRCRVFLQDHCLAEGYGTKKTSKHAAADEALKILQKTQPTYPSVKSSQCHTGSSPRGSGKKKDIKDLVVYENSSNPVCTLNDTAQFNRMTVEYVYERMTGLRWKCKVILESEVIAEAVGVKKTVKYEAAGEAVKTLKKTQPTVINNLKKGAVEDVISRNEIQGRSAEEAYKQQIKEDNIGNQLLRKMGWTGGGLGKSGEGIREPISVKEQHKREGLGLDVERVNKIAKRDIEQIIRNYARSESHTDLTFSRELTNDERKQIHQIAQKYGLKSKSHGVGHDRYLVVGRKRRKEDLLDQLKQEGQVGHYELVMPQAN","proteinLength":"Full Length","predictedMolecularWeight":"104.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":690,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"O15226","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NKRF also known as NF-kappa-B repressing factor functions as a transcriptional repressor. This protein weighs approximately 85 kDa and appears in various tissues with higher expression in the spleen thymus and lungs. NKRF interacts with NF-kB elements in DNA inhibiting the transcription of specific genes especially those related to immune responses and inflammation.
Biological function summary

NKRF plays an important role in modulating inflammation and immune responses. It forms part of a complex that controls the transcriptional activity of NF-kappa-B an important transcription factor in these processes. By repressing unwanted NF-kappa-B signal NKRF prevents excessive immune response and inflammation contributing to cellular homeostasis and protecting tissues from damage.

Pathways

NKRF functions prominently in the NF-kappa-B signaling and inflammatory pathways. Within these pathways it regulates the activity of related proteins such as RelA/p65 which are components of the NF-kappa-B family. NKRF balances the inflammation by downregulating the genes activated by NF-kB making sure the response stays controlled.

NKRF is critical in conditions associated with chronic inflammation. High or low levels of NKRF correlate with diseases such as asthma and rheumatoid arthritis. In asthma NKRF may modulate the activity of pro-inflammatory cytokines while in rheumatoid arthritis it may interact with upstream molecules like IKK complex to control inflammation.
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Specifications

Form

Liquid

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General info

Function

Enhances the ATPase activity of DHX15 by acting like a brace that tethers mobile sections of DHX15 together, stabilizing a functional conformation with high RNA affinity of DHX15 (PubMed : 12381793). Involved in the constitutive silencing of the interferon beta promoter, independently of the virus-induced signals, and in the inhibition of the basal and cytokine-induced iNOS promoter activity (PubMed : 12381793). Also involved in the regulation of IL-8 transcription (PubMed : 12381793). May also act as a DNA-binding transcription regulator : interacts with a specific negative regulatory element (NRE) 5'-AATTCCTCTGA-3' to mediate transcriptional repression of certain NK-kappa-B responsive genes (PubMed : 10562553).

Subcellular localisation

Nucleus

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Product protocols

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Target data

Enhances the ATPase activity of DHX15 by acting like a brace that tethers mobile sections of DHX15 together, stabilizing a functional conformation with high RNA affinity of DHX15 (PubMed : 12381793). Involved in the constitutive silencing of the interferon beta promoter, independently of the virus-induced signals, and in the inhibition of the basal and cytokine-induced iNOS promoter activity (PubMed : 12381793). Also involved in the regulation of IL-8 transcription (PubMed : 12381793). May also act as a DNA-binding transcription regulator : interacts with a specific negative regulatory element (NRE) 5'-AATTCCTCTGA-3' to mediate transcriptional repression of certain NK-kappa-B responsive genes (PubMed : 10562553).
See full target information NKRF
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Product promise

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