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AB152305

Recombinant Human Nkx2.5 protein (GST tag N-Terminus)

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(1 Publication)

Recombinant Human Nkx2.5 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 324 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

CSX, NKX2.5, NKX2E, NKX2-5, Homeobox protein Nkx-2.5, Cardiac-specific homeobox, Homeobox protein CSX, Homeobox protein NK-2 homolog E

1 Images
SDS-PAGE - Recombinant Human Nkx2.5 protein (GST tag N-Terminus) (AB152305)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Nkx2.5 protein (GST tag N-Terminus) (AB152305)

12.5% SDS-PAGE analysis of ab152305 stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA, SDS-PAGE

applications

Biologically active

No

Accession

P52952

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB97355

Anti-Nkx2.5 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Nkx2.5 antibody (AB97355)

  • 3
  • 4
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MFPSPALTPTPFSVKDILNLEQQQRSLAAAGELSARLEATLAPSSCMLAAFKPEAYAGPEAAAPGLPELRAELGRAPSPAKCASAFPAAPAFYPRAYSDPDPAKDPRAEKKELCALQKAVELEKTEADNAERPRARRRRKPRVLFSQAQVYELERRFKQQRYLSAPERDQLASVLKLTSTQVKIWFQNRRYKCKRQRQDQTLELVGLPPPPPPPARRIAVPVLVRDGKPCLGDSAPYAPAYGVGLNPYGYNAYPAYPGYGGAACSPGYSCTAAYPAGPSPAQPATAAANNNFVNFGVGDLNAVQSPGIPQSNSGVSTLHGIRAW","proteinLength":"Full Length","predictedMolecularWeight":"61.38 kDa","actualMolecularWeight":null,"aminoAcidEnd":324,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P52952","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nkx2.5 also known as NKX2-5 or CSX is a homeobox-containing transcription factor with a molecular mass of approximately 35 kDa. It is primarily expressed in the heart specifically in cardiac progenitor cells and also found in low levels in the spleen stomach and pancreas. As a transcription factor Nkx2.5 plays a significant role in regulating the expression of genes critical for cardiac development and differentiation. The protein's expression is tightly regulated during early embryogenesis and maintained in the heart throughout life.
Biological function summary

Nkx2.5 functions to direct the development of cardiac myocytes by regulating genes involved in cell specification and tissue formation. Nkx2.5 does not solely operate but interacts with other cardiac-specific transcription factors such as GATA4 and TBX5 collectively forming a transcriptional complex. This cooperative interaction is essential in orchestrating the genetic program necessary for heart morphogenesis and cellular proliferation within the developing myocardium.

Pathways

The protein Nkx2.5 significantly influences cardiogenesis and heart formation pathways. Its activity is necessary in the Wnt signaling pathway important for the initial stages of cardiac cell lineage differentiation. Additionally Nkx2.5 works in tandem with the protein MEF2C to regulate cardiac-specific genes ensuring the proper development and functioning of cardiac tissues. Both pathways highlight important regulatory networks where Nkx2.5 is an important player in heart development.

Mutations or dysregulations of Nkx2.5 can lead to congenital heart defects such as atrial septal defects and tetralogy of Fallot. These conditions often arise due to disrupted genetic signaling during heart formation. Nkx2.5 mutations have been associated with conduction system defects in conjunction with the GATA4 protein both known to be involved in maintaining normal cardiac structure and function. Understanding Nkx2.5's role in these diseases aides in developing potential therapeutic approaches targeting congenital heart abnormalities.
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Specifications

Form

Liquid

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General info

Function

Transcription factor required for the development of the heart and the spleen (PubMed : 22560297). During heart development, acts as a transcriptional activator of NPPA/ANF in cooperation with GATA4 (By similarity). May cooperate with TBX2 to negatively modulate expression of NPPA/ANF in the atrioventricular canal (By similarity). Binds to the core DNA motif of NPPA promoter (PubMed : 22849347, PubMed : 26926761). Together with PBX1, required for spleen development through a mechanism that involves CDKN2B repression (PubMed : 22560297). Positively regulates transcription of genes such as COL3A1 and MMP2, resulting in increased pulmonary endothelial fibrosis in response to hypoxia (PubMed : 29899023).

Sequence similarities

Belongs to the NK-2 homeobox family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Transcription factor required for the development of the heart and the spleen (PubMed : 22560297). During heart development, acts as a transcriptional activator of NPPA/ANF in cooperation with GATA4 (By similarity). May cooperate with TBX2 to negatively modulate expression of NPPA/ANF in the atrioventricular canal (By similarity). Binds to the core DNA motif of NPPA promoter (PubMed : 22849347, PubMed : 26926761). Together with PBX1, required for spleen development through a mechanism that involves CDKN2B repression (PubMed : 22560297). Positively regulates transcription of genes such as COL3A1 and MMP2, resulting in increased pulmonary endothelial fibrosis in response to hypoxia (PubMed : 29899023).
See full target information NKX2-5
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Computational and mathematical methods in medicine 2021:6959557 PubMed34912471

2021

lncENST Suppress the Warburg Effect Regulating the Tumor Progress by the Nkx2-5/ErbB2 Axis in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Geng Chen,Jiayun Jiang,Xiaofei Wang,Kai Feng,Kuansheng Ma
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Product promise

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