Recombinant Human Nkx3.1 protein (denatured) is a Human Full Length protein, in the 1 to 234 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M L R V P E P R P G E A K A E G A A P P T P S K P L T S F L I Q D I L R D G A Q R Q G G R T S S Q R Q R D P E P E P E P E P E G G R S R A G A Q N D Q L S T G P R A A P E E A E T L A E T E P E R H L G S Y L L D S E N T S G A L P R L P Q T P K Q P Q K R S R A A F S H T Q V I E L E R K F S H Q K Y L S A P E R A H L A K N L K L T E T Q V K I W F Q N R R Y K T K R K Q L S S E L G D L E K H S S L P A L K E E A F S R A S L V S V Y N S Y P Y Y P Y L Y C V G S W S P A F W
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. Plays an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Acts as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells.
Alternative names
NKX3.1, NKX3A, NKX3-1, Homeobox protein Nkx-3.1, Homeobox protein NK-3 homolog A
Recommended products
Recombinant Human Nkx3.1 protein (denatured) is a Human Full Length protein, in the 1 to 234 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q99801-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 12.01% Urea, 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M L R V P E P R P G E A K A E G A A P P T P S K P L T S F L I Q D I L R D G A Q R Q G G R T S S Q R Q R D P E P E P E P E P E G G R S R A G A Q N D Q L S T G P R A A P E E A E T L A E T E P E R H L G S Y L L D S E N T S G A L P R L P Q T P K Q P Q K R S R A A F S H T Q V I E L E R K F S H Q K Y L S A P E R A H L A K N L K L T E T Q V K I W F Q N R R Y K T K R K Q L S S E L G D L E K H S S L P A L K E E A F S R A S L V S V Y N S Y P Y Y P Y L Y C V G S W S P A F W
- Accession
- Q99801
- Protein length
- Full Length
- Predicted molecular weight
- 28.7 kDa
- Amino acids
- 1 to 234
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. Plays an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Acts as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells.
- Sequence similarities
Belongs to the NK-3 homeobox family.
- Post-translational modifications
Ubiquitinated by TOPORS; monoubiquitinated at several residues and also polyubiquitinated on single residues.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Nkx3.1 known also as NK-3 transcription factor related locus 1 is a protein that acts as a transcription factor. Its molecular mass is approximately 25 kDa. The expression of Nkx3.1 occurs mostly in the prostate but you can also see it in other tissues like testis and certain brain regions. It plays roles in androgen receptor signaling where it binds to specific DNA sequences to regulate the transcription of target genes influencing cell growth and differentiation.
- Biological function summary
Nkx3.1 is involved in regulating prostate epithelial cell differentiation and maintaining proper glandular architecture. It does not appear to form part of a larger protein complex but instead interacts directly with DNA to control gene expression. Alongside this it may involve co-regulatory proteins to execute its transcriptional activities effectively controlling the expression of genes critical for normal prostate function.
- Pathways
Nkx3.1 is involved in the prostate cancer pathway and the androgen receptor signaling pathway. Within these pathways its interactions include those with proteins such as the androgen receptor (AR) and the homeobox protein Nkx2-5 coordinating the cellular responses to hormonal signals. These interactions allow Nkx3.1 to modulate the transcription of genes responsive to androgens which impacts cellular proliferation and development within prostate tissues.
- Associated diseases and disorders
Alterations in the Nkx3.1 protein function relate most notably to prostate cancer and benign prostatic hyperplasia (BPH). Loss or mutation of the Nkx3.1 gene often coincides with early stages of prostate cancer development. Through the androgen receptor pathway Nkx3.1 influences disease progression where decreased expression or activity can exacerbate cellular abnormalities. Understanding its altered interactions in these diseases particularly with the androgen receptor provides insight into mechanisms driving prostate disorders offering potential targets for therapeutic intervention.
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SDS-PAGE - Recombinant Human Nkx3.1 protein (denatured) (ab139222)
15% SDS-PAGE analysis of 3 µg ab139222.
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